Abstract: A bioactive formulation including ethanolic concentrates of Andrographolide extract in range of 90% by wt-99% by wt and aqueous 2 hydroxypropyl beta cyclodextrin in the range of 2% by wt-10% by wt. A method for preparation of the bioactive formulation including the following steps: mixing Andrographolide extract and ethanol to prepare ethanolic concentrates of Andrographolide (solvent A), mixing hydroxypropyl beta cyclodextrin and ethanol to obtain an ethanolic concentrate 2 hydroxypropyl beta cyclodextrin (Solvent B), mixing the solvent A and solvent B to obtain a complex concentrate solution, recovering ethanol solvent from the concentrate solution through distillation to obtain a thick paste of the concentrate, drying the paste at a temperature in the range of 55° C.-65° C. and pressure in the range of ?650 to 700 mm Hg to obtain a dried complex concentrate followed by converting into an amorphous powder to obtain a bioactive formulation.