Abstract: This invention provides a method for culturing dermal papilla cells with at least either of the mammalian epidermal cells from the sole or other portions of a mammal and the conditioned medium thereof, in order to permit long stable subculture of dermal papilla cells while keeping the original function thereof intact.

Abstract: This invention provides a method for preparing hybridomas, which include the steps of: quickly and deeply freezing hair-bulbs of follicle isolated from a human or an animal of species A; immunizing an animal of species B by subcutaneously administering the hair-bulbs after being thawed out to the animal; re-immunizing the animal by intravenously and intraperitoneally administering liquid and solid components of hair-bulbs obtained by centrifugation of minced thawed hair-bulbs to the animal; fusing spleen cells or lymphoid cells isolated from the immunized animal with myeloma cells derived from an animal of species B or C, and cloning fusion cells which produce antibodies capable of specifically binding to the hair follicle of human or animal of species A.