Patents by Inventor Myeong-Je Cho
Myeong-Je Cho has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210002657Abstract: Methods of detecting the impacts on plant health attributable to the presence of one or more agronomically important polypeptides of interest in a transgenic plant are disclosed. The methods involve transforming plants or plant cells with nucleic acid sequences encoding proteins of agronomically important traits. The transformed plants or plant cells expressing the nucleic acid sequences encoding the proteins of agronomically important traits are compared to transformed plants or plant cells expressing a neutral control gene to detect the impacts on plant health attributable to the presence of the one or more agronomically important polypeptides of interest.Type: ApplicationFiled: February 28, 2019Publication date: January 7, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: STEVEN HENRY BASS, HYEON-JE CHO, MYEONG-JE CHO, VIRGINIA CRANE, MATTHEW J HECKERT, JIAN JIN, TODD J JONES, KEVIN E MCBRIDE, JEANNE SANDAHL, SHIV BAHADUR TIWARI
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Publication number: 20170198266Abstract: Compositions and transformation methods to increase the frequency of plants in a population of transformed plants which have a single copy of the target polynucleotide of interest are provided. The frequency of plants in a population of transformed plants containing no contaminating vector backbone sequence may be increased. The methods and compositions provide for a greater number of transgenic events having single copy inserts and no contaminating vector backbone sequence.Type: ApplicationFiled: June 5, 2015Publication date: July 13, 2017Applicant: Pioneer HI Breed Internaional Inc.Inventors: Ajith Anand, Myeong-Je Cho, William Gordon-Kamm
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Patent number: 9273321Abstract: The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.Type: GrantFiled: October 17, 2014Date of Patent: March 1, 2016Assignee: PIONEER HI BRED INTERNATIONAL INC.Inventors: Myeong-Je Cho, David C. Cerf, Deping Xu, Zuo-Yu Zhao
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Publication number: 20150037807Abstract: The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.Type: ApplicationFiled: October 17, 2014Publication date: February 5, 2015Inventors: Myeong-Je Cho, David C. Cerf, Deping Xu, Zuo-Yu Zhao
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Publication number: 20140173775Abstract: Compositions and methods are provided for the production and selection of transgenic plants and plant parts, for increasing the transformation frequency of a plant or plant part, and for regulating the expression of a transgene, such as a herbicide tolerance polynucleotide. The methods and compositions allow for the delay in the expression of herbicide tolerance polynucleotides until a point in development during which herbicide selection is more efficient. Compositions comprise polynucleotide constructs comprising an excision cassette that separates a transgene, such as a herbicide tolerance polynucleotide, from its promoter and host cells comprising the same. The excision cassette comprises a polynucleotide encoding a site-specific recombinase operably linked to an inducible promoter and expression of the recombinase leads to excision of the excision cassette and expression of the transgene.Type: ApplicationFiled: March 13, 2013Publication date: June 19, 2014Applicant: Pioneer Hi-Bred International, Inc.Inventors: Myeong-Je Cho, Samuel R. Ellis, William J. Gordon-Kamm, Zuo-Yo Zhoa
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Publication number: 20140173781Abstract: Compositions and methods are provided for the production and selection of transgenic plants and plant parts, for increasing the transformation frequency of a plant or plant part, and for regulating the expression of a transgene, such as a herbicide tolerance polynucleotide. The methods and compositions allow for the delay in the expression of herbicide tolerance polynucleotides until a point in development during which herbicide selection is more efficient. Compositions comprise polynucleotide constructs comprising an excision cassette that separates a transgene, such as a herbicide tolerance polynucleotide, from its promoter and host cells comprising the same. The excision cassette comprises a polynucleotide encoding a site-specific recombinase operably linked to an inducible promoter and expression of the recombinase leads to excision of the excision cassette and expression of the transgene.Type: ApplicationFiled: March 13, 2013Publication date: June 19, 2014Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Myeong-Je Cho, Samuel R. Ellis, William James Gordon-Kamm, Zuo-Yu Zhao
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Patent number: 8404930Abstract: The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.Type: GrantFiled: January 26, 2010Date of Patent: March 26, 2013Assignee: Pioneer Hi-Bred International, Inc.Inventors: Xinli E. Wu, Myeong-Je Cho, Zuo-Yu Zhao
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Publication number: 20130055472Abstract: Compositions and methods for the efficient transformation and regeneration of monocot plants are provided. The methods of transformation involve infection with Agrobacterium. In this manner, any gene of interest can be introduced into the monocot plant with high transformation efficiency and in low copy number. Transformed and regenerated monocot cells, tissues, plants, and seed are also provided. The invention encompasses regenerating transformed plants, transgenic seeds produced therefrom, and transgenic plants and transgenic seeds from subsequent generations.Type: ApplicationFiled: August 30, 2012Publication date: February 28, 2013Applicants: E.I. du Pont de Nemours and Company, Pioneer Hi Bred International Inc.Inventors: Myeong-Je Cho, Theodore M. Klein, Zuo-Yu Zhao
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Publication number: 20110165561Abstract: The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.Type: ApplicationFiled: December 23, 2010Publication date: July 7, 2011Applicant: Pioneer Hi-Bred International, Inc.Inventors: Myeong-Je Cho, David C. Cerf, Deping Xu, Zuo-Yu Zhao
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Publication number: 20100192253Abstract: The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.Type: ApplicationFiled: January 26, 2010Publication date: July 29, 2010Applicant: PIONEER HI-BRED, INC.Inventors: XINLI E. WU, MYEONG-JE CHO, ZUO-YU ZHAO
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Patent number: 7179964Abstract: The present invention is directed to a transgenic plant wherein at least a part of said plant includes a recombinant nucleic acid with a promoter active in the part operably linked to a nucleic acid encoding a thioredoxin polypeptide wherein the promoter is a seed or grain maturation-specific promoter and the thioredoxin polypeptide includes the amino acid sequence WCGPC. The present invention is further directed to transgenic plants that overexpress thioredoxin in seed wherein the overexpression of thioredoxin h effects a significant increase in the reduction of proteins (—SH as compared to S—S) of the albumin fraction of the seed.Type: GrantFiled: July 12, 2002Date of Patent: February 20, 2007Assignee: The Regents of the University of CaliforniaInventors: Myeong-Je Cho, Joshua H. Wong, Peggy G. Lemaux, Bob B. Buchanan
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Patent number: 7157629Abstract: Transgenic monocot plants and seeds comprising monocot seed-specific promoters from seed-storage protein genes and monocot seed-specific targets sequences of expression of heterologous proteins in subcellular compartments to protect the protein are disclosed. The transgenic monocot plants include maize, wheat, rice and barley. Also disclosed are methods of producing transgenic monocot seed from the transgenic plants.Type: GrantFiled: June 13, 2003Date of Patent: January 2, 2007Assignee: The Regents of the University of CaliforniaInventors: Myeong-Je Cho, Peggy G. Lemaux, Bob B. Buchanan
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Patent number: 7102056Abstract: Improved compositions and methods for transformation and regeneration of plants from embryogenic callus are disclosed that include, for example: use of an intermediate-incubation medium after callus induction to increase the competence of the transformed cells for regeneration; dim light conditions during early phases of selection; use of green callus tissue as a target for microprojectile bombardment; and media with optimized levels of phytohormones and copper concentrations.Type: GrantFiled: April 18, 2000Date of Patent: September 5, 2006Assignee: The Regents of the University of CaliforniaInventors: Peggy G. Lemaux, Myeong-Je Cho
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Publication number: 20050015831Abstract: Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.Type: ApplicationFiled: March 25, 2004Publication date: January 20, 2005Applicant: The Regents of the University of CaliforniaInventors: Myeong-Je Cho, Peggy Lemaux, Bob Buchanan, Joshua Wong, Corina Marx
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Patent number: 6833493Abstract: The present invention provides barley thioredoxin h nucleic acids and NADP-thioredoxin reductase nucleic acids, the respective encoded proteins and methods of use.Type: GrantFiled: March 5, 2002Date of Patent: December 21, 2004Assignee: The Regents of the University of CaliforniaInventors: Myeong-Je Cho, Gregorio del Val, Maxime Caillau, Peggy G. Lemaux, Bob B. Buchanan
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Patent number: 6784346Abstract: Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.Type: GrantFiled: March 29, 2000Date of Patent: August 31, 2004Assignee: The Regents of the University of CaliforniaInventors: Myeong-Je Cho, Peggy G. Lemaux, Bob B. Buchanan, Joshua Wong, Corina Marx
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Publication number: 20040088754Abstract: Methods for producing proteins in plant seeds are disclosed. Expression of the protein is driven by a seed-specific promoter and the protein is preferably expressed as a fusion polypeptide that includes a signal peptide that causes the protein to accumulate in a subcellular compartment to protect the protein. Also disclosed are improved methods for transforming plants, including cereals such as barley and wheat.Type: ApplicationFiled: June 13, 2003Publication date: May 6, 2004Inventors: Myeong-Je Cho, Peggy G. Lemaux, Bob B. Buchanan
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Patent number: 6642437Abstract: Methods for producing proteins in plant seeds are disclosed. Expression of the protein is driven by a seed-specific promoter and the protein is preferably expressed as a fusion polypeptide that includes a signal peptide that causes the protein to accumulate in a subcellular compartment to protect the protein.Type: GrantFiled: September 30, 1998Date of Patent: November 4, 2003Assignee: The Regents of the University of CaliforniaInventors: Peggy G. Lemaux, Myeong-Je Cho, Bob B. Buchanan
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Publication number: 20030150010Abstract: The present invention provides barley thioredoxin h nucleic acids and NADP-thioredoxin reductase nucleic acids, the respective encoded proteins and methods of use.Type: ApplicationFiled: March 5, 2002Publication date: August 7, 2003Inventors: Myeong-Je Cho, Gregorio del Val, Maxime Caillau, Peggy G. Lemaux, Bob B. Buchanan
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Publication number: 20030135878Abstract: The present invention is directed to a transgenic plant wherein at least a part of said plant includes a recombinant nucleic acid with a promoter active in the part operably linked to a nucleic acid encoding a thioredoxin polypeptide wherein the promoter is a seed or grain maturation-specific promoter and the thioredoxin polypeptide includes the amino acid sequence WCGPC. The present invention is further directed to transgenic plants that overexpress thioredoxin in seed wherein the overexpression of thioredoxin h effects a significant increase in the reduction of proteins (—SH as compared to S—S) of the albumin fraction of the seed.Type: ApplicationFiled: July 12, 2002Publication date: July 17, 2003Inventors: Myeong-Je Cho, J. H. Wong, Peggy G. Lemaux, Bob B. Buchanan