Patents by Inventor Myriam-Laure Cubizolles

Myriam-Laure Cubizolles has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200158604
    Abstract: A system for preparing a calibrated volume of blood plasma that includes a plurality of parts: a device for preparing the calibrated volume of blood plasma, which is in the form of a single card; a pneumatic-type actuating system SP; a treatment and command unit UC configured such as to execute a command sequence of different pneumatic command points of the pneumatic actuation system with a view to obtaining, from a taken blood sample, the calibrated volume of blood plasma. The device includes three distinct modules: a fluidic connection module M1 onto which a blood sampling device may be connected; a module M2 for separation of the blood plasma contained in the blood sample; a module M3 for obtaining at least one calibrated volume of blood plasma, after separation.
    Type: Application
    Filed: November 14, 2019
    Publication date: May 21, 2020
    Applicant: Commissariat A L'Energie Atomique et aux Energies Alternatives
    Inventors: Myriam-Laure Cubizolles, Manuel Alessio, Remco Den Dulk, Frederic Revol-Cavalier
  • Patent number: 10407711
    Abstract: The present invention relates to a method of obtaining peptides from procaryotic and/or eucaryotic cells.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: September 10, 2019
    Assignee: bioMérieux
    Inventors: Marie-Hélène Charles, Jean-Philippe Charrier, Myriam-Laure Cubizolles, Agnès Dupont-Filliard, Véronique Lanet, Florence Rivera, Laurent Veron, Lucie Baujard-Lamotte, Christine Peponnet
  • Patent number: 10346983
    Abstract: The invention is a method for estimating the amount of analyte in a fluid sample, and in particular in a bodily fluid. The sample is mixed with a reagent able to form a color indicator in the presence of the analyte. The sample is then illuminated by a light beam produced by a light source; an image sensor forms an image of the beam transmitted by the sample, from which image a concentration of the analyte in the fluid is estimated. The method is intended to be implemented in compact analyzing systems. One targeted application is the determination of the glucose concentration in blood.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: July 9, 2019
    Assignees: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AU ENERGIES ALTERNATIVES, AVALUN
    Inventors: Jean-Guillaume Coutard, Patrick Pouteau, Myriam Laure Cubizolles
  • Publication number: 20190112632
    Abstract: The present invention relates to a method of obtaining peptides from procaryotic and/or eucaryotic cells.
    Type: Application
    Filed: December 18, 2018
    Publication date: April 18, 2019
    Applicants: bioMérieux, Commissariat à l'Energie Atomique et aux Energies Alternatives
    Inventors: Marie-Hélène CHARLES, Jean-Philippe CHARRIER, Myriam-Laure CUBIZOLLES, Agnès DUPONT-FILLIARD, Véronique LANET, Florence RIVERA, Laurent VERON, Lucie BAUJARD-LAMOTTE, Christine PEPONNET
  • Patent number: 10190148
    Abstract: The present invention relates to a method of obtaining peptides from procaryotic and/or eucaryotic cells.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: January 29, 2019
    Assignees: bioMérieux, Commissariat à l'Energie Atomique et aux Energies Alternatives
    Inventors: Marie-Hélène Charles, Jean-Philippe Charrier, Myriam-Laure Cubizolles, Agnès Dupont-Filliard, Véronique Lanet, Florence Rivera, Laurent Veron, Lucie Baujard-Lamotte, Christine Peponnet
  • Publication number: 20190012788
    Abstract: The invention is a method for estimating the amount of analyte in a fluid sample, and in particular in a bodily fluid. The sample is mixed with a reagent able to form a color indicator in the presence of the analyte. The sample is then illuminated by a light beam produced by a light source; an image sensor forms an image of the beam transmitted by the sample, from which image a concentration of the analyte in the fluid is estimated. The method is intended to be implemented in compact analyzing systems. One targeted application is the determination of the glucose concentration in blood.
    Type: Application
    Filed: July 10, 2018
    Publication date: January 10, 2019
    Applicants: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, AVALUN
    Inventors: Jean-Guillaume COUTARD, Patrick POUTEAU, Myriam Laure CUBIZOLLES
  • Patent number: 10126315
    Abstract: The method is provided for characterizing agglomeration of particles in a liquid containing an analyte, including introducing liquid into a fluid chamber; mixing the liquid with a bifunctional reagent, lighting the fluid chamber using an excitation light beam extending through the fluid chamber in a longitudinal direction (X); acquiring at least one image using a matrix photodetector, each image including pixels (In(x,y), x,y representing the coordinates of a pixel of an image, the image (I(x,y)) being formed by radiation transmitted by the lighted fluid chamber; and calculating from at least one acquired image (I(x,y)), at least one indicator (Ind2) characterizing the particle agglomeration. The photodetector can be positioned less than 1 cm from the fluid chamber, and during the calculation step, the calculated indicator (Ind2) is representative of the intensity of the pixels of the image. The method advantageously allows for characterizing the agglomeration of particles in a liquid.
    Type: Grant
    Filed: April 8, 2016
    Date of Patent: November 13, 2018
    Assignee: COMMISSARIAT À L'ÉNERGIE ATOMIQUE ET AUX ÉNERGIES ALTERNATIVES
    Inventors: Vincent Poher, Myriam-Laure Cubizolles, Patrick Pouteau, Cédric Allier, Johanna Spiaczka
  • Patent number: 10049453
    Abstract: The invention is a method for estimating the amount of analyte in a fluid sample, and in particular in a bodily fluid. The sample is mixed with a reagent able to form a color indicator in the presence of the analyte. The sample is then illuminated by a light beam produced by a light source; an image sensor forms an image of the beam transmitted by the sample, from which image a concentration of the analyte in the fluid is estimated. The method is intended to be implemented in compact analyzing systems. One targeted application is the determination of the glucose concentration in blood.
    Type: Grant
    Filed: July 7, 2016
    Date of Patent: August 14, 2018
    Assignees: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, AVALUN
    Inventors: Jean-Guillaume Coutard, Patrick Pouteau, Myriam Laure Cubizolles
  • Patent number: 9921147
    Abstract: The invention is a method for characterizing a liquid sample, said liquid sample containing particles, the method comprising the following steps: a) illuminating said sample using a light source that is able to emit an incident light wave towards the sample; b) detecting, using a photodetector, a light wave transmitted by the sample thus illuminated; c) characterizing the sample depending on an intensity of the light wave detected by the photodetector. The method comprises, prior to step c), applying an acoustic wave to the sample, said acoustic wave forming pressure nodes and pressure antinodes in the sample, so as to separate, in the latter, a poor portion, poor in particles, and rich portion, rich in particles, such that, in step c), the sample is characterized: either on the basis of the intensity of the light wave transmitted by the poor portion; or on the basis of the intensity of the light wave transmitted by the rich portion.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: March 20, 2018
    Assignee: Commissariat A L'Energie Atomique et aux Energies Alternatives
    Inventors: Vivian Aubert, Myriam-Laure Cubizolles, Cedric Poulain, Maxime Huet, David Rabaud
  • Publication number: 20170268989
    Abstract: The invention is a method for characterizing a liquid sample, said liquid sample containing particles, the method comprising the following steps: a) illuminating said sample using a light source that is able to emit an incident light wave towards the sample; b) detecting, using a photodetector, a light wave transmitted by the sample thus illuminated; c) characterizing the sample depending on an intensity of the light wave detected by the photodetector. The method comprises, prior to step c), applying an acoustic wave to the sample, said acoustic wave forming pressure nodes and pressure antinodes in the sample, so as to separate, in the latter, a poor portion, poor in particles, and rich portion, rich in particles, such that, in step c), the sample is characterized: either on the basis of the intensity of the light wave transmitted by the poor portion; or on the basis of the intensity of the light wave transmitted by the rich portion.
    Type: Application
    Filed: March 16, 2017
    Publication date: September 21, 2017
    Applicant: Commissariat A L'Energie Atomique et aux Energies Alternatives
    Inventors: Vivian AUBERT, Myriam-Laure CUBIZOLLES, Cedric POULAIN, Maxime HUET, David RABAUD
  • Publication number: 20170011517
    Abstract: The invention is a method for estimating the amount of analyte in a fluid sample, and in particular in a bodily fluid. The sample is mixed with a reagent able to form a color indicator in the presence of the analyte. The sample is then illuminated by a light beam produced by a light source; an image sensor forms an image of the beam transmitted by the sample, from which image a concentration of the analyte in the fluid is estimated. The method is intended to be implemented in compact analyzing systems. One targeted application is the determination of the glucose concentration in blood.
    Type: Application
    Filed: July 7, 2016
    Publication date: January 12, 2017
    Applicants: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, AVALUN
    Inventors: Jean-Guillaume COUTARD, Patrick POUTEAU, Myriam Laure CUBIZOLLES
  • Patent number: 9506935
    Abstract: This method for estimating the quantity of an analyte contained in a liquid includes the following steps: introducing the liquid into a fluid chamber; mixing the liquid with a bi-specific reagent, the bi-specific reagent being configured for grafting on both a particle and an analyte present in the liquid; lighting the fluid chamber using an excitation beam emitted by a light source, the beam extending through the fluid chamber; acquiring at least one image using a matrix photodetector, the image being formed by radiation transmitted by the lighted fluid chamber; and estimating, from at least one acquired image, the quantity of said analyte in the liquid.
    Type: Grant
    Filed: December 4, 2014
    Date of Patent: November 29, 2016
    Assignee: COMMISSARIAT À L'ÉNERGIE ATOMIQUE ET AUX ÉNERGIES ALTERNATIVES
    Inventors: Maxime Huet, Vincent Poher, Myriam-Laure Cubizolles, Patrick Pouteau, Cédric Allier, Johanna Spiaczka
  • Publication number: 20160299158
    Abstract: The method is provided for characterizing agglomeration of particles in a liquid containing an analyte, including introducing liquid into a fluid chamber; mixing the liquid with a bifunctional reagent, lighting the fluid chamber using an excitation light beam extending through the fluid chamber in a longitudinal direction (X); acquiring at least one image using a matrix photodetector, each image including pixels (In(x,y), x,y representing the coordinates of a pixel of an image, the image (I(x,y)) being formed by radiation transmitted by the lighted fluid chamber; and calculating from at least one acquired image (I(x,y)), at least one indicator (Ind2) characterizing the particle agglomeration. The photodetector can be positioned less than 1 cm from the fluid chamber, and during the calculation step, the calculated indicator (Ind2) is representative of the intensity of the pixels of the image. The method advantageously allows for charactering the agglomeration of particles in a liquid.
    Type: Application
    Filed: April 8, 2016
    Publication date: October 13, 2016
    Inventors: Vincent POHER, Myriam-Laure CUBIZOLLES, Patrick POUTEAU, Cédric ALLIER, Johanna SPIACZKA
  • Patent number: 9341636
    Abstract: The method according to the invention is capable of characterizing a variation in the speed of particles or agglomeration of particles, the particles, such as blood particles, being contained in a liquid (12). The characterization method includes the following steps: introducing the liquid (12) into a fluid chamber (14); lighting the fluid chamber (14) using an excitation light beam (18) emitted by a light source (16), the light beam (18) extending through the fluid chamber (14) in a longitudinal direction (X); acquiring at least one image using a matrix photodetector (20), the image being formed by radiation transmitted by the lighted fluid chamber (14); and calculating, from at least one acquired image, at least one indicator characterizing the variation of the speed or agglomeration of the particles. During the acquisition step, the photodetector (20) is positioned at a distance (D2) smaller than 1 cm from the fluid chamber (14) in the longitudinal direction (X).
    Type: Grant
    Filed: May 30, 2013
    Date of Patent: May 17, 2016
    Assignee: Commissariat à l'énergie atomique et aux énergies alternatives
    Inventors: Vincent Poher, Myriam-Laure Cubizolles, Patrick Pouteau, Cédric Allier, Johanna Spiaczka
  • Publication number: 20150160244
    Abstract: This method for estimating the quantity of an analyte contained in a liquid includes the following steps: introducing the liquid into a fluid chamber; mixing the liquid with a bi-specific reagent, the bi-specific reagent being configured for grafting on both a particle and an analyte present in the liquid; lighting the fluid chamber using an excitation beam emitted by a light source, the beam extending through the fluid chamber; acquiring at least one image using a matrix photodetector, the image being formed by radiation transmitted by the lighted fluid chamber; and estimating, from at least one acquired image, the quantity of said analyte in the liquid.
    Type: Application
    Filed: December 4, 2014
    Publication date: June 11, 2015
    Inventors: Maxime HUET, Vincet Poher, Myriam-Laure Cubizolles, Patrick Pouteau, Cédric Allier, Johanna Spiaczka
  • Publication number: 20150087003
    Abstract: The present invention relates to a method of obtaining peptides from procaryotic and/or eucaryotic cells.
    Type: Application
    Filed: May 2, 2013
    Publication date: March 26, 2015
    Applicants: bioMérieux, Commissariat à I'Energie Atomique et aux Energies Alternatives
    Inventors: Marie-Hélène Charles, Jean-Philippe Charrier, Myriam-Laure Cubizolles, Agnès Dupont-Filliard, Véronique Lanet, Florence Rivera, Laurent Veron
  • Patent number: 8699777
    Abstract: A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.
    Type: Grant
    Filed: April 28, 2010
    Date of Patent: April 15, 2014
    Assignee: Commissariat a l'energie Atomique et aux Energies Alternatives
    Inventors: Magalie Faivre, Patrice Caillat, Myriam-Laure Cubizolles, Christine Peponnet, Claude Vauchier
  • Publication number: 20130323757
    Abstract: The method according to the invention is capable of characterizing a variation in the speed of particles or agglomeration of particles, the particles, such as blood particles, being contained in a liquid (12). The characterization method includes the following steps: introducing the liquid (12) into a fluid chamber (14); lighting the fluid chamber (14) using an excitation laser beam (18) emitted by a light source (16), the laser beam (18) extending through the fluid chamber (14) in a longitudinal direction (X); acquiring at least one image using a matrix photodetector (20), the image being formed by radiation transmitted by the lighted fluid chamber (14); and calculating, from at least one acquired image, at least one indicator characterizing the variation of the speed or agglomeration of the particles. During the acquisition step, the photodetector (20) is positioned at a distance (D2) smaller than 1 cm from the fluid chamber (14) in the longitudinal direction (X).
    Type: Application
    Filed: May 30, 2013
    Publication date: December 5, 2013
    Inventors: Vincent POHER, Myriam-Laure Cubizolles, Patrick Pouteau, Cédric Allier, Johanna Spiaczka
  • Patent number: 8426156
    Abstract: The present invention relates to a method for assaying catalytic plasma enzymes, such as transaminases and NADH-dependent enzymes, in a sample of whole blood, by measuring, in a microfluidic chamber, the decrease in the fluorescence of NADH consumed during the enzymatic reactions catalyzed by the NADH-dependent enzymes.
    Type: Grant
    Filed: April 20, 2010
    Date of Patent: April 23, 2013
    Assignee: Commissariat a l'Energie Atomique et aux Energies Alternatives
    Inventors: Myriam-Laure Cubizolles, Marie-Line Cosnier, Magalie Faivre, Patrick Pouteau
  • Publication number: 20110104712
    Abstract: The present invention relates to a method for assaying catalytic plasma enzymes, such as transaminases and NADH-dependent enzymes, in a sample of whole blood, by measuring, in a microfluidic chamber, the decrease in the fluorescence of NADH consumed during the enzymatic reactions catalyzed by the NADH-dependent enzymes.
    Type: Application
    Filed: April 20, 2010
    Publication date: May 5, 2011
    Inventors: Myriam-Laure Cubizolles, Marie-Line Cosnier, Magali Faivre, Patrick Pouteau