Abstract: A cellularized nerve regeneration graft is disclosed that includes an electrospun biodegradable polymer conduit having an exterior surface and an interior luminal space, a plurality of fibroblasts seeded to the exterior surface of the conduit, and a system filling the interior luminal space of the conduit. The system may include a hydrogel matrix or augmented hydrogel matrix and Schwann cells. The cellularized nerve regeneration graft may be used in the repair of peripheral nerve injuries. Methods of making the cellularized nerve regeneration graft are also disclosed.

Abstract: A biocompatible sleeve designed to encase an assembly of small 3D muscles that have been cultured in vitro. The sleeve is formed from polymer fibers in such a way that pushing the two ends of the sleeve towards each other increases the diameter of the sleeve so as to facilitate insertion of the engineered muscles. Subsequent pulling at the ends of the sleeves decreases the diameter of the sleeve to facilitate a secure fit around the engineered muscle during implantation of the sleeve into a patient. The composition of the polymer fibers can be tuned to achieve the desired mechanical properties and rate of degradability.