Abstract: The disclosure provides methods of deaminating adenosine and cytosine bases in a target nucleic acid sequence in an USH2A gene comprising contacting the USH2A gene with a base editor in association with a guide RNA (gRNA). In some aspects, base editing is used to restore US2HA function by disrupting a splice site in the USH2A gene sequence to induce skipping of an exon containing a mutation, while in other embodiments, base editing is used to restore US2HA function by correcting a point mutation e.g., in an exon) so as to correct mutations. The disclosure also provides complexes of adenosine base editors and guide RNAs, and complexes of cytidine base editors and guide RNAs. The disclosure further provides pharmaceutical compositions and cells comprising these complexes. The disclosure also provides vectors encoding these complexes, base editors, and gRNAs. In some embodiments, the methods and compositions provided herein are used to treat Usher syndrome and autosomal recessive retinitis pigmentosa (arRP).

Abstract: Airbag configurations and systems are provided to protect a vehicle occupant in an oblique crash event. In one embodiment an airbag includes first and second side panels and a main panel coupled to the first side panel along a first seam and coupled to the second side panel along a second seam. The main panel can include a curved configuration to match expansion of the second side panel. The airbag configuration is provided for protection for an oblique crash event to absorb energy from occupant movement in the oblique direction and to retain and reduce head rotation of an occupant. In one embodiment, a method for controlling an airbag system is provided that includes detecting a crash event and activation of an airbag and a side curtain airbag (SCAB). The SCAB may be activated to contact the airbag and to control volume/loading of the airbag.

Abstract: Airbag configurations and systems are provided to protect a vehicle occupant in an oblique crash event. In one embodiment an airbag includes first and second side panels and a main panel coupled to the first side panel along a first seam and coupled to the second side panel along a second seam. The main panel can include a curved configuration to match expansion of the second side panel. The airbag configuration is provided for protection for an oblique crash event to absorb energy from occupant movement in the oblique direction and to retain and reduce head rotation of an occupant. In one embodiment, a method for controlling an airbag system is provided that includes detecting a crash event and activation of an airbag and a side curtain airbag (SCAB). The SCAB may be activated to contact the airbag and to control volume/loading of the airbag.

Abstract: A rear vehicle seat airbag module configured to be mounted to a portion of a seat back of a front vehicle seat comprising an inflator and an inflatable cushion comprising a lower portion, an upper portion and an external tether connecting the lower portion and the upper portion. The inflator provides inflation gas to the inflatable cushion and the inflatable cushion is configured to deploy forward of an occupant seated in a rear seat of a vehicle by curving away from the occupant. The external tether is configured to control a trajectory of the deployment of the inflatable cushion by pulling the upper portion toward the lower portion to curve the inflatable cushion such that the upper portion is located above the lower portion.

Abstract: A rear vehicle seat airbag module configured to be mounted to a portion of a seat back of a front vehicle seat comprising an inflator and an inflatable cushion comprising a lower portion, an upper portion and an external tether connecting the lower portion and the upper portion. The inflator provides inflation gas to the inflatable cushion and the inflatable cushion is configured to deploy forward of an occupant seated in a rear seat of a vehicle by curving away from the occupant. The external tether is configured to control a trajectory of the deployment of the inflatable cushion by pulling the upper portion toward the lower portion to curve the inflatable cushion such that the upper portion is located above the lower portion.