Abstract: A base element of a multi-chamber biochip has a bottom with a frame situated thereon, open on its side facing away from the bottom, wherein an interior space is enclosed by the frame. Furthermore, at least one first web is present; however, preferably a first web and a second web are present. Culture chambers formed by the webs and the frame, and delimited from one another via membranes, are contacted via channels, and serve to generate biological model systems. The base element is in particular formed as a single piece (monolithically). A multi-chamber biochip, a set, and a method for making a multi-chamber biochip are also disclosed.

Abstract: A cell culture chamber is for the in vitro production and cultivation of cell layers and organ models with two first and second channels arranged one above the other and separated from one another by a porous membrane with two side surfaces and through which flow can pass, wherein a cell substrate is formed in each case by the side surfaces of the membrane. The cell culture chamber is characterized in that at least the inner walls of the first and the second channels consist of polybutylene terephthalate. Further, a method is for cultivating human or animal cells, and in particular liver sinusoidal endothelial cells, alone and in co-culture with hepatocytes and immune cells.