Abstract: This invention relates to the use of the Aspergillus niger aspergilloglutamic peptidase in a feed composition containing cereals, pulses, oilseeds, and/or tubers to improve animal performance, reduce nitrogen excretion and/or reduce intestinal inflammation. Furthermore, the present invention relates to a feed composition or a feed additive comprising the Aspergillus niger aspergilloglutamic peptidase. The present invention relates to the discovery that the Aspergillus niger aspergilloglutamic peptidase is capable of efficiently hydrolyzing alpha-amylase/trypsin inhibitors that are present in wheat, barley and related cereal species, as well as hydrolyzing trypsin inhibitors that are present in pulses, oilseeds, and tubers.