Abstract: cDNA clones coding for TGF-.beta.2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF-.beta.2, as well as precursor TGF-.beta.2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfected COS cells are described. CHO and COS transfectants secreting TGF-.beta.2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF-.beta.2 precursor secrete approximately 5 .mu.g per ml culture media.

Abstract: A combination cultivator, rake and hoe having cultivator tines of uneven length parallel to one another and inclined with respect to the handle. A hoe blade, preferably with a sharpened forward edge is connected on one or both ends of the row of cultivator tines extending substantially parallel to the tines and to the handle. Rake tines directed oppositely to the cultivator tines are offset upwardly toward the handle. The uneven lengths of the cultivator tines reduce the intitial force needed to be applied to penetrate the soil and the blade or blades exert substantially no drag when the tines are moved forwardly or backwardly through the soil. The hoe blade or blades can be used with the tool rotated 90.degree. from the cultivating position. The blade is pushed against the stem of weeds or other growth to be removed, slightly below the soil surface.