Abstract: Disclosed is a method of stimulating adult corneal endothelial cell proliferation, which includes incubation of corneal endothelium to a mitogen-containing medium and then to an agent that interrupts cell-cell contacts in the adult corneal endothelial cells, and subsequently, as needed, further incubation in the mitogen-containing medium. The cell-cell contact-interrupting agent is preferably a calcium chelator.

Abstract: A human cell growth medium formulation for culturing human cells of neural crest origin, most preferably corneal endothelial cells, or for accelerating the growth and proliferation of human cells of neural crest origin is disclosed. The formulation for the nutrient medium of the invention includes nerve growth factor, preferably at a concentration of 1-100 ng/ml and most preferably 20 ng/ml. The growth medium formulation preferably also includes epidermal growth factor (preferably at a concentration of 1-200 ng/ml and most preferably 5 ng/ml).

Abstract: The composition and method of using a non-steroidal antiinflammatory compound, such as indomethacin and/or a growth factor that causes a change in cell function when bound to specific cell surface receptor, such as epidermal growth factor (EGF), to enhance healing and restore function of the corneal endothelium. The compositions can be administered alone, in combination, or in sequence, before or after injury, for example, in an irrigation solution or in combination with a nutritive solution for storage of cornea prior to transplant.

Abstract: Method using a non-steroidal antiinflammatory compound, such as indomethacin and/or a growth factor that causes a change in cell function when bound to a specific cell surface receptor, such as epidermal growth factor (EGF), to enhance healing and restore function of the corneal endothelium. The compositions can be adminsitered alone, in combination, or in sequence, before or after injury, for example, in an irrigation solution or in combination with a nutritive solution for storage of a cornea prior to transplant.Examples of useful growth factors include, in addition to EGF and EGF-like compounds, fragments of EGF containing the receptor binding region, and EGF modified to improve stability, increase half-life, or enhance permeation into the eye. Examples of other non-steroidal antiinflammatories include aspirin, ibuprofen, and suprofen. The composition can be applied topically to the eye or delivered in a controlled fashion by means of a material such as a polymeric implant, gel, or liposomes.

Abstract: Method using a non-steroidal antiinflammatory compound, such as indomethacin and/or a growth factor that causes a change in cell function when bound to a specific cell surface receptor, such as epidermal growth factor (EGF), to enhance healing and restore function of the corneal endothelium. The compositions can be administered alone, in combination, or in sequence, before or after injury, for example, in an irrigation solution or in combination with a nutritive solution for storage of a cornea prior to transplant.Examples of useful growth factors include, in addition to EGF and EGF-like compounds, fragments of EGF containing the receptor binding region, and EGF modified to improve stability, increase half-life, or enhance permeation into the eye. Examples of other non-steroidal antiinflammatories include aspirin, ibuprofen, and suprofen. The composition can be applied topically to the eye or delivered in a controlled fashion by means of a material such as a polymeric implant, gel, or liposomes.