Patents by Inventor Nancy E. Harding
Nancy E. Harding has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8652792Abstract: The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient Sphingans. The invention is also directed to a process for preparing a clarified Sphingan solution comprising heating aqueous Sphingan solution, in particular PUB-deficient Sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified Sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.Type: GrantFiled: November 1, 2010Date of Patent: February 18, 2014Assignee: C.P. Kelco U.S., Inc.Inventors: Stan Bower, Ellen Burke, Nancy E. Harding, Yamini N. Patel, J. Carrie Schneider, Dagmar Meissner, Neil A. Morrison, Ralph Bezanson
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Publication number: 20130203153Abstract: The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene 1 Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.Type: ApplicationFiled: January 4, 2011Publication date: August 8, 2013Inventors: Nancy E. Harding, Yamini N. Patel, Russell J. Coleman
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Publication number: 20130084607Abstract: The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.Type: ApplicationFiled: August 30, 2012Publication date: April 4, 2013Applicant: CP KELCO U.S., INC.Inventors: Nancy E. Harding, Yamini N. Patel, Russell Coleman, Steven Matzke
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Publication number: 20130059363Abstract: The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.Type: ApplicationFiled: August 30, 2012Publication date: March 7, 2013Applicant: CP KELCO U.S., INC.Inventors: Nancy E. Harding, Yamini N. Patel, Russell Coleman, Steven Matzke
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Patent number: 8278438Abstract: The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials.Type: GrantFiled: September 28, 2010Date of Patent: October 2, 2012Assignee: CP Kelco U.S., Inc.Inventors: Nancy E. Harding, Yamini N. Patel, Russell Coleman, Steven Matzke
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Patent number: 8198064Abstract: The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.Type: GrantFiled: December 2, 2005Date of Patent: June 12, 2012Assignee: CP Kelco U.S., Inc.Inventors: Stan Bower, Ellen Burke, Nancy E. Harding, Yamini N. Patel, J. Carrie Schneider, Dagmar Meissner, Neil A. Morrison, Ralph Bezanson
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Publication number: 20110281334Abstract: The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene M or gene N Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.Type: ApplicationFiled: January 4, 2011Publication date: November 17, 2011Inventors: Nancy E. Harding, Yamini N. Patel, Russell J. Coleman
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Publication number: 20110282051Abstract: The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials.Type: ApplicationFiled: September 28, 2010Publication date: November 17, 2011Applicant: C P Kelco U.S., Inc.Inventors: Nancy E. Harding, Yamini N. Patel, Russell Coleman, Steven Matzke
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Publication number: 20110171721Abstract: The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.Type: ApplicationFiled: January 4, 2011Publication date: July 14, 2011Applicant: CP KELCO U.S., INC.Inventors: Nancy E. Harding, Yamini N. Patel, Russell J. Coleman
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Patent number: 7887866Abstract: The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.Type: GrantFiled: March 2, 2001Date of Patent: February 15, 2011Assignee: CP Kelco U.S., Inc.Inventors: Stan Bower, Ellen Burke, Nancy E. Harding, Yamini N. Patel, J. Carrie Schneider, Dagmar Meissner, Neil A. Morrison, Ralph Bezanson
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Patent number: 7888333Abstract: The present invention provides improved diutan polysaccharides produced by mutant Sphingomonas strains containing at least one genetic modification that favors slime-forming diutan production that exhibits increased viscosity. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.Type: GrantFiled: February 3, 2006Date of Patent: February 15, 2011Assignee: CP Kelco U.S., Inc.Inventors: Nancy E. Harding, Yamini N. Patel, Russell J. Coleman
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Publication number: 20110027838Abstract: PHB-deficient Sphingomonas strains having improved sphingan yield are provided. Certain of the Sphingomonas strains are diutan-producing strains that exhibit a dramatic improvement in productivity and yield due to a combination of certain genetic modifications that affect PHB and sphingan synthesis. Moreover, the sphingans produced from such strains have superior characteristics including improved filterability, clarity, and improved rheology-modifying characteristics. The sphingans provided are, thus, highly desirable in a variety of commercial and industrial uses, including personal care items, cement applications, and oilfield applications.Type: ApplicationFiled: July 31, 2009Publication date: February 3, 2011Inventors: Nancy E. Harding, Todd A. Talashek, Yamini N. Patel
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Publication number: 20110009611Abstract: The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.Type: ApplicationFiled: March 2, 2001Publication date: January 13, 2011Inventors: Stan Bower, Ellen Burke, Nancy E. Harding, Yamini N. Patel, J. Carrie Schneider, Dagmar Meissner, Neil A. Morrison, Ralph Bezanson
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Patent number: 7868167Abstract: The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials.Type: GrantFiled: November 1, 2005Date of Patent: January 11, 2011Assignee: CP Kelco U.S., Inc.Inventors: Nancy E. Harding, Yamini N. Patel, Russell Coleman, Steven Matzke
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Patent number: 7829697Abstract: The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.Type: GrantFiled: December 2, 2005Date of Patent: November 9, 2010Assignee: CP Kelco US, Inc.Inventors: Stan Bower, Ellen Burke, Nancy E. Harding, Yamini N. Patel, J. Carrie Schneider, Dagmar Meissner, Neil A. Morrison, Ralph Bezanson
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Publication number: 20080319186Abstract: The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials.Type: ApplicationFiled: November 1, 2005Publication date: December 25, 2008Inventors: Nancy E. Harding, Yamini N. Patel, Russell Coleman, Steven Matzke
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Publication number: 20080268527Abstract: The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.Type: ApplicationFiled: December 2, 2005Publication date: October 30, 2008Applicant: C.P. Kelco U.S., Inc.Inventors: Stan Bower, Ellen Burke, Nancy E. Harding, Yamini N. Patel, J. Carrie Schneider, Dagmar Meissner, Neil A. Morrison, Ralph Bezanson
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Patent number: 7361754Abstract: Mutational inactivation of proteins involved in para-cresol production in certain milk products results in improved taste and odor. The undesirable para-cresol forms over time as a result of enzymes produced by the bacterium that produces gellan gum. Since the gellan is typically used in a relatively unpurified form, the enzymes are added to the milk along with the gellan. Inactivation of the enzymes is a genetic means of eliminating the enzymes without requiring any additional purification or processing.Type: GrantFiled: June 20, 2005Date of Patent: April 22, 2008Assignee: CP Kelco US, Inc.Inventors: Joseph M. Cleary, Russell J. Coleman, Nancy E. Harding, Yamini N. Patel
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Publication number: 20030100078Abstract: This invention provides a mutant Sphingomonas microorganism which produces a polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-glucuronic acid:L-rhamnose ratio of about 2:1:1, wherein the D-glucose moieties are linked in a &bgr;-[1,4] configuration to the D-glucuronic acid moiety. One of the D-glucose moieties is linked to the L-rhamnose moiety in an &agr;-[1,3] configuration, the other glucose moiety is linked to the L-rhamnose moiety in a &bgr;-[1,4] configuration, wherein the polysaccharide polymer is substantially non-acetylated. This invention also provides the substantially non-acetylated polymer, as well as process for obtaining the substantially non-acetylated polymer.Type: ApplicationFiled: July 3, 2002Publication date: May 29, 2003Inventors: Nancy E. Harding, Yamini N. Patel, John O. McQuown