Abstract: The present invention relates to methods of bioassay for detecting the effective concentration of Granulocyte Colony Stimulating Factor (G-CSF), and estimating ED50 and specific activity of G-CSF using a human acute myeloid cell line. The invention also relates to use of this technique for estimating the potency of G-CSF samples during various stages of a purification process.

Abstract: The invention consists of a stable preparation of a combination drug, comprising of an anti-inflammatory agent and an anti-infective agent. The anti-inflammatory agent in this invention is a corticosteroid, and the anti-infective agent is a derivative of quinolone, amino-glycoside or their pharmaceutically acceptable salts. The combination drug essentially comprises of i) an anti-inflammatory agent which is a corticosteroid, ii) an anti-infective agent selected from the group comprising of derivatives of quinolone, aminoglycoside and their pharmaceutically acceptable salts; iii) a complexation enhancing polymer; iv) a solubiliser exhibiting an inclusion phenomenon, along with pharmaceutically acceptable excipients with a suitable carrier system.