Abstract: Methods, systems, and devices for screening chemotherapeutic drug(s) on patient specific tissue and the related methods of treating cancer are provided. Candidate chemotherapeutic drug(s) may be tested on samples of resected tumor and/or tumor-adjacent normal tissue from the patient ex-vivo. In some cases, the tumor tissue and tumor-adjacent normal tissue samples are separately co-cultured ex-vivo in the presence of immune cells isolated from the same patient. The tumor and tumor-adjacent normal tissue is evaluated post-treatment for markers of efficacy of the candidate regimens. A chemotherapeutic regimen(s) that provide optimal treatment of the patient's specific cancer may then be considered by the oncologist to be administered to the patient.