Abstract: An isolated, porcine ?1-6 fucosyltransferase having the following physico-chemical properties: (1) action: transferring fucose from guanosine diphosphate-fucose to a hydroxy group at 6-position of GluNAc closest to R of a receptor (GlcNAc?1-2Man?1-6)(GlcNAc?1-2Man?1-3)Man?1-4GlcNAc?1-4GlucNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc?1-2Man?1-6)-(GlcNAc?1-2Man?1-3)Man?1-4GlcNAc?1-4(Fuc?1-6)GlucNAc-R (2) optimum pH: about 7.0 (3) pH stability: retains activity after 5 hours of treatment at 4° C. at a pH range of 4.0-10.0 (4) optimum temperature: about 30-37° C. (5) inhibition or activation: no requirement for divalent metal for expression of activity; no inhibition of activity in the presence of 5 mM EDTA (6) molecular weight: about 60,000 by SDS-polyacrylamide gel electrophoresis is provided. An isolated polynucleotide encoding porcine ?1-6 fucosyltransferase is also provided.

Abstract: An isolated, porcine &agr;1-6 fucosyltransferase having the following physico-chemical properties:

Abstract: Alpha-1-6 fucosyltransferases with a human or swine origin which have the following function; genes encoding these enzymes; expression vectors containing these genes; transformants prepared by using these expression vectors; and a process for producing a recombinant ±1-6 fucosyltransferase by incubating such a transformant.

Abstract: Porcine- or human-derived &agr;1-6 fucosyltransferases having the following action: action: transferring fucose from guanosine diphosphate-fucose to the hydroxy group at 6-position of GluNAc closest to R of a receptor (GlcNAc&bgr;1-2Man&agr;1-6) (GlcNAc&bgr;1-2Man&agr;1-3) Man&bgr;1-4GlcNAc&bgr;1-4GlucNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc&bgr;1-2Man&agr;1-6)-(GlcNAc&bgr;1-2Man&agr;1-3)Man&bgr;1-4GlcNAc&bgr;1-4(Fuc&agr;1-6)GlucNAc-R; a gene encoding these enzymes; an expression vector containing the gene; a transformant prepared by using this expression vector; and a method for producing a recombinant &agr;1-6 fucosyltransferase, by culturing the transformant.

Abstract: Porcine- or human-derived .alpha.1-6 fucosyltransferases having the following action:action: transferring fucose from guanosine adiphosphate-fucose to the hydroxy group at 6-position of GlcNAc closest to R of a receptor (GlcNAc.beta.1-2Man.alpha.1-6)(GlcNAc.beta.1-2Man.alpha.1-3)Man.beta.1-4Gl cNAc.beta.1-4GlcNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc.beta.1-2Man.alpha.1-6)-(GlcNAc.beta.1-2Man.alpha.1-3)Man.beta.1-4G lcNAc.beta.1-4(Fuc.alpha.1-6)GlcNAc-R; a gene encoding these enzymes; an expression vector containing the gene; a transformant prepared by using this expression vector; and a method for producing a recombinant .alpha.1-6 fucosyltransferase, by culturing the transformant.