Patents by Inventor Naokazu Naito

Naokazu Naito has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for secretory production of protein
    Patent number: 5824502
    Abstract: Efficient secretory production of a recombinant protein in the periplasm can be attained by artificially regulating co-expression of a gene coding for the target recombinant protein and a gene coding for Sec protein involved in protein transport in E. coli cells under conditions in which excessive expression of the Sec protein is suppressed.
    Type: Grant
    Filed: March 27, 1997
    Date of Patent: October 20, 1998
    Assignee: Mitsui Chemicals, Inc.
    Inventors: Masaru Honjo, Naokazu Naito, Hiroshi Uchida
  • Method for secretory production of protein
    Patent number: 5759810
    Abstract: In producing a recombinant protein in the periplasm of Escherichia coli, the productivity of the target ecombinant protein can be promoted by coexpressing glutathione reductase artificially with the target recombinant protein.
    Type: Grant
    Filed: March 28, 1996
    Date of Patent: June 2, 1998
    Assignee: Mitsui Toatsu Chemicals, Inc.
    Inventors: Masaru Honjo, Naokazu Naito, Hiroshi Uchida
  • Process for producing 20 kD human growth hormone
    Patent number: 5496713
    Abstract: A human growth hormone having a molecular weight of 20,000 daltons can be effectively secreted and produced in the periplasm of Escherichia coli by constructing a human growth hormone secretion plasmid comprising a vector DNA replicable in E. coli and a DNA fragment including a promoter, a ribosome binding site, a secretion signal coding region, which are all those of the neutral protease gene of B. amyloliquefaciens and a gene encoding 20 kD hGH placed just downstream from the secretion signal coding region; introducing the said plasmid into E. coli; and culturing the resultant transformed cells.
    Type: Grant
    Filed: September 9, 1993
    Date of Patent: March 5, 1996
    Assignee: Mitsui Toatsu Chemicals, Inc.
    Inventors: Masaru Honjo, Setsuo Yoshino, Akira Nakayama, Naokazu Naito