Abstract: The invention aims to provide an immunoglobulin-binding protein having improved chemical stability, especially stability against alkali. The object can be achieved by improving stability against alkali by substituting an amino acid residue(s) at a particular position(s) in an immunoglobulin-binding domain such as domain C of protein A derived from a bacterium belonging to the genus Staphylococcus, to another/other particular amino acid residue(s).

Abstract: The present application addresses the problem of providing an Fc-binding protein having an improved antibody separation ability. The present application also addresses the problem of providing a high-accuracy antibody separation method using an insoluble carrier having the protein immobilized thereon. The problems can be solved by: an Fc-binding protein in which at least an amino acid substitution at a specific position therein occurs and which has reduced affinity for an antibody; and an antibody separation method including allowing an equilibration buffer solution to pass through a column in which an insoluble carrier having the protein immobilized thereon is filled to equilibrate the column, adding a solution containing an antibody to cause the adsorption of the antibody onto the carrier, and eluting the antibody adsorbed on the carrier using an elution solution.

Abstract: The present invention addresses the problem of providing FcRn having improved stability with respect to heat and acids, a method for producing said FcRn, an antibody adsorbent in which said FcRn is used, and an antibody isolation method in which said adsorbent is used. The above problem is solved by substituting an amino acid residue at a specific position in an extracellular region of a human FcRn ? chain and/or a ?2 microglobulin region of a human FcRn ? chain by another specific amino acid.

Abstract: The problem to be addressed by the present invention is to provide improved recombinant Fc?RIIb and Fc?RIIa that do not require refolding and exhibit high productivity and thermal stability, and to provide a method for producing the same. Said problem is solved by improved recombinant Fc?RIIb comprising at least the amino acid residues of the extracellular domain of human Fc?RIIb (No. 43 to No. 215 in UniProt No. P31994), wherein, in said amino acid residues, at least one amino acid substitution has occurred at a position corresponding to No. 82, 94, 98, 104, 105, or 139 in UniProt No. P31994. Said problem is also solved by improved recombinant Fc?RIIa comprising at least the amino acid residues of the extracellular domain of human Fc?RIIa (No. 34 to No. 206 in UniProt No. P12318-1), wherein, in said amino acid residues, at least one amino acid substitution has occurred at a position corresponding to No. 73, 85, 89, 95, 96, or 130 in UniProt No. P12318-1.

Abstract: There is disclosed a display device making it possible to call effective attention in a use situation where unbalanced passenger load occurs between left and right lanes of a passenger conveyor. This display device includes a first display unit (7A) positioned on the left and a second display unit (7B) positioned on the right toward the passenger conveyor at a boarding (1), a control device (20) to control the first display unit (7A) and the second display unit (7B), and passenger detection (8A, 8B) to detect passengers stepping on the passenger conveyor. The first display unit (7A) and the second display unit (7B) display images representing information to passengers. The passengers pass between the first display unit (7A) and the second display unit (7B) and step on the passenger conveyor.

Abstract: There is disclosed a display device making it possible to call effective attention in a use situation where unbalanced passenger load occurs between left and right lanes of a passenger conveyor. This display device includes a first display unit (7A) positioned on the left and a second display unit (7B) positioned on the right toward the passenger conveyor at a boarding (1), a control device (20) to control the first display unit (7A) and the second display unit (7B), and passenger detection means (8A, 8B) to detect passengers stepping on the passenger conveyor. The first display unit (7A) and the second display unit (7B) display images representing information to passengers. The passengers pass between the first display unit (7A) and the second display unit (7B) and step on the passenger conveyor.

Abstract: The invention aims to provide an immunoglobulin-binding protein having improved chemical stability, especially stability against alkali. The object can be achieved by improving stability against alkali by substituting an amino acid residue(s) at a particular position(s) in an immunoglobulin-binding domain such as domain C of protein A derived from a bacterium belonging to the genus Staphylococcus, to another/other particular amino acid residue(s).

Abstract: A vise for column, for attaching a plug to a column tube of a column for chromatography comprising the column tube and the plug to be attached to one end of the column tube, is provided. The vise comprises a column supporter configured to be able to support a part of the column for chromatography, a main body configured to be connected to the column supporter and extending from the column supporter by a predetermined distance, and a column presser configured to be supported at another end of the main body opposite to one end to which the column supporter is connected, and configured to be movable toward and away from the column supporter, and configured to be able to press the plug against the column tube.

Abstract: A vise for column, for attaching a plug to a column tube of a column for chromatography comprising the column tube and the plug to be attached to one end of the column tube, is provided. The vise comprises a column supporter configured to be able to support a part of the column for chromatography, a main body configured to be connected to the column supporter and extending from the column supporter by a predetermined distance, and a column presser configured to be supported at another end of the main body opposite to one end to which the column supporter is connected, and configured to be movable toward and away from the column supporter, and configured to be able to press the plug against the column tube.

Abstract: Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.

Abstract: The present invention addresses the problem of providing FcRn having improved stability with respect to heat and acids, a method for producing said FcRn, an antibody adsorbent in which said FcRn is used, and an antibody isolation method in which said adsorbent is used. The above problem is solved by substituting an amino acid residue at a specific position in an extracellular region of a human FcRn ? chain and/or a ?2 microglobulin region of a human FcRn ? chain by another specific amino acid.

Abstract: The present application addresses the problem of providing an Fc-binding protein having an improved antibody separation ability. The present application also addresses the problem of providing a high-accuracy antibody separation method using an insoluble carrier having the protein immobilized thereon. The problems can be solved by: an Fc-binding protein in which at least an amino acid substitution at a specific position therein occurs and which has reduced affinity for an antibody; and an antibody separation method including allowing an equilibration buffer solution to pass through a column in which an insoluble carrier having the protein immobilized thereon is filled to equilibrate the column, adding a solution containing an antibody to cause the adsorption of the antibody onto the carrier, and eluting the antibody adsorbed on the carrier using an elution solution.

Abstract: A column filling apparatus mainly includes a tank, a stirrer being an agitator, a pump, a resin bottle, first to eleventh pipes, first to fourth valves composed of three-way valves, and fifth and sixth valves composed of two-way valves. A suction port is mounted on a leading end of the fifth pipe, and the suction port is inserted into an inner part from a top opening of the resin bottle. The other end of the fifth pipe is connected to the first valve. The first valve is connected to the first pipe, the second pipe, and the fifth pipe, and connects or disconnects these pipes to or from each other. An end of the first pipe is mounted near a bottom of the tank, and is opened near an inner bottom surface of the tank.

Abstract: A vise for column, for attaching a plug to a column tube of a column for chromatography comprising the column tube and the plug to be attached to one end of the column tube, is provided. The vise comprises a column supporter configured to be able to support a part of the column for chromatography, a main body configured to be connected to the column supporter and extending from the column supporter by a predetermined distance, and a column presser configured to be supported at another end of the main body opposite to one end to which the column supporter is connected, and configured to be movable toward and away from the column supporter, and configured to be able to press the plug against the column tube.

Abstract: The problem to be addressed by the present invention is to provide improved recombinant Fc?RIIb and Fc?RIIa that do not require refolding and exhibit high productivity and thermal stability, and to provide a method for producing the same. Said problem is solved by improved recombinant Fc?RIIb comprising at least the amino acid residues of the extracellular domain of human Fc?RIIb (No. 43 to No. 215 in UniProt No. P31994), wherein, in said amino acid residues, at least one amino acid substitution has occurred at a position corresponding to No. 82, 94, 98, 104, 105, or 139 in UniProt No. P31994. Said problem is also solved by improved recombinant Fc?RIIa comprising at least the amino acid residues of the extracellular domain of human Fc?RIIa (No. 34 to No. 206 in UniProt No. P12318-1), wherein, in said amino acid residues, at least one amino acid substitution has occurred at a position corresponding to No. 73, 85, 89, 95, 96, or 130 in UniProt No. P12318-1.

Abstract: Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.

Abstract: Described herein are methods of identifying compounds that can modulate the transport of a steroid hormone across a phospholipid membrane. Also described herein are methods of identifying compounds that can affect the transcriptional activity of a steroid hormone nuclear receptor.

Abstract: Described herein are methods of identifying compounds that can modulate the transport of a steroid hormone across a phospholipid membrane. Also described herein are methods of identifying compounds that can affect the transcriptional activity of a steroid hormone nuclear receptor.

Abstract: Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.

Abstract: Provided are a floating brake disk assembling method and a floating brake disk, and a floating brake disk assembling device, wherein a washer or a spring member can be accurately attached to a coupling pin. In the floating disk brake assembling method, a coupling pin 32 for coupling a brake disk (10) to a hub disk (20), which has a cylindrical body portion (41) to be mounted in a coupling hole (31), a first jaw portion (42) formed in one end portion of the body portion (41), and a portion to be swaged (43A) continuous to the other end portion of the body portion (41), is used; and the portion to be swaged (43A) is swaged so that a second jaw portion (42) having a diameter larger than that of the body portion (41) can be formed.