Abstract: An object of the present disclosure is to implement, at a low cost, highly accurate color calibration in a printing apparatus that performs printing using fluorescent ink. One embodiment of the present invention is an image processing apparatus that includes: a processing unit configured to perform processing relating to color calibration in a printing apparatus that prints an image on a printing medium using fluorescent printing material; and an execution unit configured to perform calibration processing of the printing apparatus based on a measured value that is obtained by measuring a patch chart for obtaining information on an amount of application of the fluorescent printing material in the printing apparatus, wherein a patch included in the patch chart is printed with the fluorescent printing material and subtractive color mixture printing material and at least one dot of the subtractive color mixture printing material covers a dot of the fluorescent printing material.

Abstract: A mRNA for the synthesis of a protein, including a translation region containing a start codon and a stop codon and an untranslated region positioned on the 5?-end side of the start codon, in which some phosphate groups within the range of at least from the 5?end of the untranslated region to 15 nt on the 3?-end side of the start codon are substituted with phosphorothioate groups, is provided. A method for producing an mRNA, including: a step of preparing a DNA template; a step of preparing an unmodified NTP containing ATP, GTP, CTP, and UTP, and a modified NTP in which at least one kind of phosphate group of the unmodified NTP is substituted with a phosphorothioate group; and a step of performing a transcription reaction with RNA polymerase using the DNA template as a template and the unmodified NTP and the modified NTP as substrates, is also provided.

Abstract: A method for producing a capped RNA which is an RNA having the 5?-end modified with a cap, the method including: reacting an activated capping compound represented by with a monophosphate RNA having the 5?-end monophosphorylated, where, L represents a leaving group.

Abstract: A primer for amplifying a nucleic acid having a structure represented by formula (1): where A1 represents —S—, —S—S—, or —Se—, B represents a base, and R1 represents a decomposable protecting group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3?-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; Klenow fragment for making the 3? ends blunt; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3?-protruding end.

Abstract: A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, R1 represents a decomposable protecting group, and R2 represents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3?-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3?-protruding end.

Abstract: The present invention relates to a single-chain circular RNA having a sustained or slow-releasing RNA interference effect, characterized in that the single-chain circular RNA comprises a sense strand sequence, an antisense strand sequence complementary to the sense strand sequence, identical or different two loop sequences between the sense strand and the antisense strand, connecting both strands, wherein the sense strand and the antisense strand are paired to form a stem.

Abstract: The present invention relates to a single-chain circular RNA having a sustained or slow-releasing RNA interference effect, characterized in that the single-chain circular RNA comprises a sense strand sequence, an antisense strand sequence complementary to the sense strand sequence, identical or different two loop sequences between the sense strand and the antisense strand, connecting both strands, wherein the sense strand and the antisense strand are paired to form a stem.

Abstract: A vehicle-mounted cell stack system includes a cell stack that comprises a plurality of laminated power generating cells, and power collecting plates that sandwich both outermost power generating cells, electric wires connected to the power collecting plates, and an electric load connected to the power collecting plates via the electric wires and configured to be activated by electric power that is supplied from the cell stack. The electric wires are connected to the power collecting plates in a direction other than a cell laminating direction.

Abstract: The present invention provides a cyclic RNA preferable for carrying out rotary protein translation in which translation domains other than that of the target protein are sufficiently short and translation efficiency is high, and a method for producing protein that uses this cyclic RNA as template. More specifically, the present invention provides a cyclic RNA that encodes a protein, has a full-length number of bases that is equal to or greater than 102 and is a multiple of 3, has at least one start codon, does not have a stop codon in the same reading frame as the start codon, and does not contain an internal ribosome entry site (IRES). In addition, the present invention provides a method for producing protein in a eukaryotic cell expression system that consists of using the aforementioned cyclic RNA as template and expressing a protein encoded by that cyclic RNA.

Abstract: The present invention provides a cyclic RNA preferable for carrying out rotary protein translation in which translation domains other than that of the target protein are sufficiently short and translation efficiency is high, and a method for producing protein that uses this cyclic RNA as template. More specifically, the present invention provides a cyclic RNA that encodes a protein, has a full-length number of bases that is equal to or greater than 102 and is a multiple of 3, has at least one start codon, does not have a stop codon in the same reading frame as the start codon, and does not contain an internal ribosome entry site (IRES). In addition, the present invention provides a method for producing protein in a eukaryotic cell expression system that consists of using the aforementioned cyclic RNA as template and expressing a protein encoded by that cyclic RNA.

Abstract: A vehicle-mounted cell stack system includes a cell stack that comprises a plurality of laminated power generating cells, and power collecting plates that sandwich both outermost power generating cells, electric wires connected to the power collecting plates, and an electric load connected to the power collecting plates via the electric wires and configured to be activated by electric power that is supplied from the cell stack. The electric wires are connected to the power collecting plates in a direction other than a cell laminating direction.

Abstract: A natural leather is treated with a tanning agent, a re-tanning agent, a dye and a greasing agent, wherein a treatment agent for inhibiting the generation of formaldehyde and acetaldehyde from the natural leather is added to the greasing step so that the natural leather is impregnated with the treatment agent. The treatment agent includes a hydrazide compound optionally together with sodium hydrogen sulfite.

Abstract: This object relates to a leather which is obtained by forming a coating film through the greasing step following re-tanning and dyeing and the drying step. The purpose of this object is to provide a leather, wherein the generation of free formaldehyde and acetaldehyde is inhibited, an automobile interior part using this leather and a back sizing agent for natural leather to be used for producing the above-described leather.

Abstract: The present invention relates to a circular single-stranded nucleic acid complex comprising a sense strand RNA sequence, an antisense strand RNA sequence complementary to the sense strand RNA sequence, and two identical or different loop moieties connecting the sense and antisense strands, wherein the sense and antisense strands form a stem by pairing, and the loop moieties are selected from the group consisting of polyalkylene glycol, DNA, DNA-RNA chimera, a derivative thereof, and a combination thereof.

Abstract: This object relates to a natural leather which is obtained by forming a coating film through the greasing step following re-tanning and dyeing and the drying step. The purpose of this object is to provide a natural leather, wherein the generation of free formaldehyde and acetaldehyde is inhibited, and a treating agent to be added in the greasing step for natural leather. A natural leather wherein formaldehyde and acetaldehyde generated by a tanning agent, a re-tanning agent, a dye and a greasing agent having been incorporated into a leather are confined in the leather with a treating agent to be added in the greasing step for natural leather which contains a hydrazide compound optionally together with sodium hydrogen sulfite to thereby inhibit or prevent the generation of formaldehyde and acetaldehyde, and an automobile interior part coated with this natural leather.

Abstract: The present invention relates to a single-chain circular RNA having a sustained or slow-releasing RNA interference effect, characterized in that the single-chain circular RNA comprises a sense strand sequence, an antisense strand sequence complementary to the sense strand sequence, identical or different two loop sequences between the sense strand and the antisense strand, connecting both strands, wherein the sense strand and the antisense strand are paired to form a stem.