Patents by Inventor Naotake Ogasawara
Naotake Ogasawara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8852943Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5?-side region outside of the target region in the host DNA, a 3?-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3?-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5?-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performType: GrantFiled: March 23, 2009Date of Patent: October 7, 2014Assignee: Kao CorporationInventors: Katsutoshi Ara, Takuya Morimoto, Naotake Ogasawara
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Publication number: 20140170703Abstract: A recombinant microorganism obtained by transferring, into a host microorganism capable of producing protein or polypeptide with increased productivity, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of Bacillus subtilis genes comA, yopO, treR, yvbA, cspB, yvaN, yttP, yurK, yozA, licR, sigL, mntR, glcT, yvdE, ykvE, slr, rocR, ccpA, yaaT, yyaA, yycH, yacP, hprK, rsiX, yhdK, and ylbO, or one or more genes functionally equivalent to any of these genes have been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.Type: ApplicationFiled: November 25, 2013Publication date: June 19, 2014Applicant: KAO CORPORATIONInventors: MASATOSHI TOHATA, KAZUHISA SAWADA, KATSUYA OZAKI, KAZUO KOBAYASHI, NAOTAKE OGASAWARA
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Publication number: 20130295676Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5?-side region outside of the target region in the host DNA, a 3?-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3?-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5?-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performType: ApplicationFiled: March 23, 2009Publication date: November 7, 2013Applicant: Kao CorporationInventors: Katsutoshi ARA, Takuya MORIMOTO, Naotake OGASAWARA
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Publication number: 20110275158Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5?-side region outside of the target region in the host DNA, a 3?-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3?-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5?-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performType: ApplicationFiled: October 4, 2010Publication date: November 10, 2011Applicant: Kao CorporationInventors: Katsutoshi ARA, Takuya MORIMOTO, Naotake OGASAWARA
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Patent number: 7981659Abstract: Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.Type: GrantFiled: September 25, 2006Date of Patent: July 19, 2011Assignees: Kao Corporation, Nara Institute of Science and TechnologyInventors: Ryosuke Kadoya, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Naotake Ogasawara
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Publication number: 20110014709Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5?-side region outside of the target region in the host DNA, a 3?-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3?-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5?-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of pType: ApplicationFiled: March 23, 2009Publication date: January 20, 2011Applicant: Kao CorporationInventors: Katsutoshi Ara, Takuya Morimoto, Naotake Ogasawara
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Patent number: 7763444Abstract: It is intended to provide koji mold-origin phospholipase A2 and a DNA encoding it. Namely, phospholipase A2 comprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A2.Type: GrantFiled: June 23, 2009Date of Patent: July 27, 2010Assignees: National Institute of Technology and Evaluation, National Institute of Advanced Industrial Science and Technology, National Research Institute of BrewingInventors: Katsuhiko Kitamoto, Manabu Arioka, Shotaro Yamaguchi, Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Publication number: 20090263888Abstract: It is intended to provide koji mold-origin phospholipase A2 and a DNA encoding it. Namely, phospholipase A2 comprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A2.Type: ApplicationFiled: June 23, 2009Publication date: October 22, 2009Applicants: National Institute of Technology and Evaluation, Natinal Institute of Advanced Industrial Science and Technology, National Research Institute of BrewingInventors: Katsuhiko Kitamoto, Manabu Arioka, Shotaro Yamaguchi, Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Publication number: 20090221055Abstract: Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.Type: ApplicationFiled: September 25, 2006Publication date: September 3, 2009Inventors: Ryosuke Kadoya, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Naotake Ogasawara
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Patent number: 7348170Abstract: Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.Type: GrantFiled: December 25, 2002Date of Patent: March 25, 2008Assignees: Amano Enzyme Inc., National Institute of Advanced Industrial Science and Technology, National Institute of Technology and Evaluation, National Research Institute of BrewingInventors: Kensuke Yuuki, Atsuki Toumoto, Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Hisahara
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Publication number: 20080009039Abstract: A recombinant microorganism obtained by transferring, into a host microorganism capable of producing protein or polypeptide with increased productivity, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of Bacillus subtilis genes comA, yopO, treR, yvbA, cspB, yvaN, yttP, yurK, yozA, licR, sigL, mntR, glcT, yvdE, ykvE, sir, rocR, ccpA, yaaT, yyaA, yycH, yacP, hprK, rsiX, yhdK, and yibO, or one or more genes functionally equivalent to any of these genes have been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.Type: ApplicationFiled: November 5, 2004Publication date: January 10, 2008Applicant: Kao CorporationInventors: Masatoshi Tohata, Kazuhisa Sawada, Katsuya Ozaki, Kazuo Kobayashi, Naotake Ogasawara
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Patent number: 7285407Abstract: The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-?-1,3-glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade ?-1,3-glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having ?-1,3-glucanase activity (exo-, or endo-?-1,3-glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having ?-1,3-glucanase activity, and a method for the production of said enzymes.Type: GrantFiled: March 22, 2005Date of Patent: October 23, 2007Assignees: National Institute of Advanced Industrial Science and Technology, National Institute of Technology and EvaluationInventors: Masayuki Machida, Motoaki Sano, Misao Sunagawa, Tasuku Nakajima, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Patent number: 7186540Abstract: A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.Type: GrantFiled: December 24, 2002Date of Patent: March 6, 2007Assignees: National Institute of Advanced Indusrtial Science and Technology, National Institute of Technology and Evaluation, National Research Institute of BrewingInventors: Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara, Kotaro Ito, Kenichiro Matsushima, Yasuji Koyama
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Publication number: 20070004003Abstract: It is intended to provide koji mold-origin phospholipase A2 and a DNA encoding it. Namely, phospholipase A2 comprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A2.Type: ApplicationFiled: March 2, 2004Publication date: January 4, 2007Applicants: National Institute of Technology and Evaluation, National Institute of Advanced Industrial Science and Technology, National Research Institute BrewingInventors: Katsuhiko Kitamoto, Manabu Arioka, Shotaro Yamaguchi, Masayuki Machida, Keletsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Publication number: 20060234320Abstract: The present invention provides DNA encoding novel pyroglutamyl peptidase derived from Aspergillus oryzae, pyroglutamyl peptidase which is produced by using the DNA, and a method for producing a protein lysate with a good flavor at a high hydrolysis rate.Type: ApplicationFiled: December 26, 2002Publication date: October 19, 2006Inventors: Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara, Chikara Tokunaga, Itaru Toda, Chiaki Saitoh, Akihiro Senoh
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Publication number: 20050287634Abstract: The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-?-1,3-glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade ?-1,3-glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having ?-1,3-glucanase activity (exo-, or endo-?-1,3-glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having ?-1,3-glucanase activity, and a method for the production of said enzymes.Type: ApplicationFiled: March 22, 2005Publication date: December 29, 2005Applicants: National Institute of advanced Industrial Science and Technology, National Institute of Technology and Evaluation, National Research Institute of BrewingInventors: Masayuki Machida, Motoaki Sano, Misao Sunagawa, Tasuku Nakajima, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Publication number: 20050142650Abstract: Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.Type: ApplicationFiled: December 25, 2002Publication date: June 30, 2005Inventors: Kensuke Yuuki, Atsuki Toumoto, Masayuki Machida, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Publication number: 20040082053Abstract: A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.Type: ApplicationFiled: December 24, 2002Publication date: April 29, 2004Inventors: Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara, Kotaro Ito, Kenichiro Matsushima, Yasuji Koyama