Patents by Inventor Naoto Nemoto
Naoto Nemoto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11942125Abstract: A thermal medium and a laser recording device are provided in which medium parameters can be acquired efficiently. According to one embodiment, the thermal medium includes a photothermal conversion layer, a color development layer, a storage mechanism. The photothermal conversion layer converts an applied laser beam into heat. The color development layer develops a color by the heat converted by the photothermal conversion layer. The storage mechanism stores information related to a medium parameter including a photothermal conversion efficiency of the photothermal conversion layer.Type: GrantFiled: September 21, 2022Date of Patent: March 26, 2024Assignees: Kabushiki Kaisha Toshiba, Toshiba Infrastructure Systems & Solutions CorporationInventors: Nobuki Nemoto, Yuuichi Nakamura, Yuuma Kadokura, Naoto Mihara
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Publication number: 20240002838Abstract: Provided is a linker for both screening assessment of the candidate clones without using enzymes, and to provide an in vitro selection method using thereof. Also, provided is a high-speed photo-crosslinking linker for molecular interaction analysis and in vitro selection comprising a backbone and a side chain. The backbone comprises a solid-phase binding site located at the 5? terminus for forming a bond with a solid-phase; a solid-phase cleavage site for releasing the entire solid-phase at the site; a side chain linking site for linking a side chain; a high-speed photo-crosslinking site for linking the backbone to mRNA having a sequence complementary thereof via photo-crosslinking; and a reverse transcription initiation region located adjacent to the side chain linking site at the 3? terminus of the backbone. The side chain comprises a fluorescent label, a protein binding site located at the free terminus thereof; and a binding site with the backbone.Type: ApplicationFiled: September 15, 2023Publication date: January 4, 2024Applicant: Saitama UniversityInventors: Naoto Nemoto, Yuki Mochizuki, Shigefumi Kumachi
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Patent number: 11634705Abstract: The purpose of the present invention is to provide a method for identifying antibody CDR3 clusters using high speed in vitro screening a library selected from the group consisting of cDNA library and nucleic acid aptamer library comprising: (i) preparing a positive spherical shaped structure by binding a target molecule to a spherical shaped molecule, wherein the target molecule is immobilized on the positive spherical shaped structure, wherein the positive spherical shaped structure may contain a fluorescent label; (ii) preparing a negative spherical shaped structure, wherein the target molecule is not immobilized on the negative spherical shaped structure, wherein the negative spherical shaped structure may contain a fluorescent label; (iii) forming a positive spherical shaped conjugate or a negative spherical shaped conjugate by binding a target detecting molecule capable of binding to the target molecule to the positive spherical shaped structure or to the negative spherical structure, wherein the targetType: GrantFiled: September 18, 2020Date of Patent: April 25, 2023Assignee: EPSILON MOLECULAR ENGINEERING INC.Inventors: Naoto Nemoto, Toshiki Miyajima, Yuta Matsukawa
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Publication number: 20220227809Abstract: A peptide that specifically binds to norovirus, which is useful for detection and infection control of norovirus is provided. A norovirus-binding peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 327.Type: ApplicationFiled: May 28, 2020Publication date: July 21, 2022Applicant: Kao CorporationInventors: Yoshitaka ITO, Takuya MORIMOTO, Shigefumi KUMACHI, Naoto NEMOTO
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Publication number: 20220229058Abstract: Provided is a peptide that specifically binds to norovirus, which is useful for detection and infection control of norovirus. A norovirus-binding peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 185.Type: ApplicationFiled: May 28, 2020Publication date: July 21, 2022Applicant: Kao CorporationInventors: Yoshitaka ITO, Takuya MORIMOTO, Shigefumi KUMACHI, Naoto NEMOTO
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Publication number: 20220221458Abstract: A peptide that specifically binds to norovirus, which is useful for detection and infection control of norovirus is provided. A norovirus-binding peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 269.Type: ApplicationFiled: May 28, 2020Publication date: July 14, 2022Applicant: Kao CorporationInventors: Yoshitaka ITO, Takuya MORIMOTO, Shigefumi KUMACHI, Naoto NEMOTO
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Publication number: 20210403604Abstract: The purpose of the present invention is to provide a method for producing a peptide that interacts with a lipid bilayer, and a lipid-bilayer-penetrating peptide obtained through the method. Provided is a lipid-bilayer-penetrating peptide constructed from 10 to 100 amino acids, the peptide having an amino acid sequence that penetrates the lipid bilayer at the C-terminal, and having an amino acid sequence with at least six contiguous arginine at the N-terminal. Also provided is a construct for producing a lipid-bilayer-penetrating peptide, the construct including, from the 5? end toward the 3? end, a tag region 1, a region 1 for incorporating a fluorescent protein gene sequence, a fluorescent protein gene region, a region 2 for incorporating a fluorescent protein gene sequence, a random region, and a stop codon region, and the construct being such that the random region has the aforementioned sequences.Type: ApplicationFiled: May 7, 2021Publication date: December 30, 2021Applicant: Saitama UniversityInventors: Naoto Nemoto, Yasuhito Utsugi
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Publication number: 20210381026Abstract: The present invention provides an improved immuno-PCR method by using cDNA display comprising the steps of: immobilizing a first antibody having a binding site to a solid phase: contacting a sample fluid to said antibody to bind a target molecule in said sample fluid; contacting said target molecule to a cDNA display being composed of a backbone being composed of a double strand and a side chain having a second antigen binding site to which the second antibody is bound; and conducting polymerase chain reaction to detect said cDNA quantitatively. According to the improved immune-PCR method of the present invention, the target molecule is screened and obtained quantitatively, because it uses cDNA display being composed of one protein/peptide and one DNA.Type: ApplicationFiled: August 21, 2021Publication date: December 9, 2021Applicants: EPSILON MOLECULAR ENGINEERING INC., Saitama UniversityInventors: Naoto Nemoto, Hironori Anzai, Takeru Suzuki, Shigefumi Kumachi, Takuya Terai
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Publication number: 20210079381Abstract: The purpose of the present invention is to provide a method for identifying antibody CDR3 clusters using high speed in vitro screening a library selected from the group consisting of cDNA library and nucleic acid aptamer library comprising: (i) preparing a positive spherical shaped structure by binding a target molecule to a spherical shaped molecule, wherein the target molecule is immobilized on the positive spherical shaped structure, wherein the positive spherical shaped structure may contain a fluorescent label; (ii) preparing a negative spherical shaped structure, wherein the target molecule is not immobilized on the negative spherical shaped structure, wherein the negative spherical shaped structure may contain a fluorescent label; (iii) forming a positive spherical shaped conjugate or a negative spherical shaped conjugate by binding a target detecting molecule capable of binding to the target molecule to the positive spherical shaped structure or to the negative spherical structure, wherein the targetType: ApplicationFiled: September 18, 2020Publication date: March 18, 2021Applicant: EPSILON MOLECULAR ENGINEERING INC.Inventors: Naoto NEMOTO, Toshiki MIYAJIMA, Yuta MATSUKAWA
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Patent number: 10781442Abstract: The purpose of the present invention is to provide a high-speed in vitro screening method for any library selected from the group consisting of a cDNA display library and a nucleic acid aptamer library. This high-speed in vitro screening method involves: (i) a step for preparing positive spherical structures formed by immobilizing a target molecule on a spherical structure and negative spherical structures having no target molecules immobilized thereon; (ii) a step in which a target detection molecule, selected from the aforementioned library having a library size of greater than or equal to 1010, is bonded on each spherical structure to obtain spherical conjugates; (iii) a step in which the spherical conjugates are sorted into positive spherical conjugates and negative spherical conjugates with a cell sorter; (v) a step for supplying the nucleic acid on the surface of the sorted spherical conjugates for PCR; (vi) and a repetition step for repeating steps (i) to (v) above using DNA obtained by PCR.Type: GrantFiled: September 28, 2018Date of Patent: September 22, 2020Assignee: EPSILON MOLECULAR ENGINEERING INC.Inventors: Naoto Nemoto, Toshiki Miyajima, Yuta Matsukawa
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Publication number: 20190085322Abstract: The purpose of the present invention is to provide a high-speed in vitro screening method for any library selected from the group consisting of a cDNA display library and a nucleic acid aptamer library. This high-speed in vitro screening method involves: (i) a step for preparing positive spherical structures formed by immobilizing a target molecule on a spherical structure and negative spherical structures having no target molecules immobilized thereon; (ii) a step in which a target detection molecule, selected from the aforementioned library having a library size of greater than or equal to 1010, is bonded on each spherical structure to obtain spherical conjugates; (iii) a step in which the spherical conjugates are sorted into positive spherical conjugates and negative spherical conjugates with a cell sorter; (v) a step for supplying the nucleic acid on the surface of the sorted spherical conjugates for PCR; (vi) and a repetition step for repeating steps (ii) to (v) above using DNA obtained by PCR.Type: ApplicationFiled: September 28, 2018Publication date: March 21, 2019Applicant: EPSILON MOLECULAR ENGINEERING INC.Inventors: Naoto NEMOTO, Toshiki MIYAJIMA, Yuta MATSUKAWA
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Patent number: 9982254Abstract: The present invention improves in vitro virus synthesis efficiency and stability of mRNA derived from screened cDNA in a cDNA display method to improve the efficiency and reliability of the production of a peptide by a molecular evolutionary engineering technique. Provided is a ligand which comprises three fingers formed from antiparallel ?-sheets and a loop region intercalated between the antiparallel ?-sheets, wherein at least a fingertip part formed by the loop region of each of the fingers is bound to the target molecule, and wherein the ligand comprises the amino acid sequence of SEQ ID NO: 1. In the amino acid sequence of SEQ ID NO: 1, X7 represents an arbitrary amino acid residue that constitutes the fingertip part of each of the fingers, each numeric character represents the number of amino acid residues, and X7 and X4 are not composed of the same amino acid residues as each other.Type: GrantFiled: May 16, 2014Date of Patent: May 29, 2018Assignees: Saitama University, Nikon CorporationInventors: Naoto Nemoto, Yuuya Yotsumoto
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Publication number: 20180100146Abstract: Provided is a linker for both screening assessment of the candidate clones without using enzymes, and to provide an in vitro selection method using thereof. Also, provided is a high-speed photo-crosslinking linker for molecular interaction analysis and in vitro selection comprising a backbone and a side chain. The backbone comprises a solid-phase binding site located at the 5? terminus for forming a bond with a solid-phase; a solid-phase cleavage site for releasing the entire solid-phase at the site; a side chain linking site for linking a side chain; a high-speed photo-crosslinking site for linking the backbone to mRNA having a sequence complementary thereof via photo-cros slinking; and a reverse transcription initiation region located adjacent to the side chain linking site at the 3? terminus of the backbone. The side chain comprises a fluorescent label, a protein binding site located at the free terminus thereof; and a binding site with the backbone.Type: ApplicationFiled: September 29, 2017Publication date: April 12, 2018Inventors: Naoto Nemoto, Yuki Mochizuki, Shigefumi Kumachi
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Patent number: 9334492Abstract: A protein-immobilizing solid phase is a protein-immobilizing solid phase comprising an mRNA-nucleic acid linker-protein complex, obtained by linking the mRNA and the protein encoded by that mRNA through the nucleic acid linker, immobilized on the solid phase, wherein the nucleic acid linker has a photocleavage site and a solid phase binding site.Type: GrantFiled: April 30, 2014Date of Patent: May 10, 2016Assignees: The University of Tokyo, NIKON CORPORATIONInventors: Shingo Ueno, Naoto Nemoto, Takanori Ichiki, Hirofumi Shiono, Hisao Osawa
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Publication number: 20150031564Abstract: The present invention improves in vitro virus synthesis efficiency and stability of mRNA derived from screened cDNA in a cDNA display method to improve the efficiency and reliability of the production of a peptide by a molecular evolutionary engineering technique. Provided is a ligand which comprises three fingers formed from antiparallel ?-sheets and a loop region intercalated between the antiparallel ?-sheets, wherein at least a fingertip part formed by the loop region of each of the fingers is bound to the target molecule, and wherein the ligand comprises the amino acid sequence of SEQ ID NO: 1. In the amino acid sequence of SEQ ID NO: 1, X7 represents an arbitrary amino acid residue that constitutes the fingertip part of each of the fingers, each numeric character represents the number of amino acid residues, and X7 and X4 are not composed of the same amino acid residues as each other.Type: ApplicationFiled: May 16, 2014Publication date: January 29, 2015Applicants: Saitama University, Nikon CorporationInventors: Naoto Nemoto, Yuuya Yotsumoto
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Publication number: 20140296111Abstract: A protein-immobilizing solid phase is a protein-immobilizing solid phase comprising an mRNA-nucleic acid linker-protein complex, obtained by linking the mRNA and the protein encoded by that mRNA through the nucleic acid linker, immobilized on the solid phase, wherein the nucleic acid linker has a photocleavage site and a solid phase binding site.Type: ApplicationFiled: April 30, 2014Publication date: October 2, 2014Applicants: Nikon Corporation, The University of TokyoInventors: Shingo Ueno, Naoto Nemoto, Takanori Ichiki, Hirofumi Shiono, Hisao Osawa
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Publication number: 20140235508Abstract: A nucleic acid linker is for producing a complex of mRNA and a protein encoded by that mRNA, comprising one 3?-terminal region and two branched 5?-terminal regions, wherein the 3?-terminal region comprises a single-stranded polynucleotide segment able to hybridize with the sequence on the 3?-terminal side of the mRNA, and an arm segment that branches off from the single-stranded polynucleotide segment and has a linking segment with the protein on the terminal thereof, and one of the two 5?-terminal regions has a binding site with the 3?-terminal of the mRNA.Type: ApplicationFiled: April 30, 2014Publication date: August 21, 2014Applicants: National University Corporation Saitama University, Nikon CorporationInventors: Naoto Nemoto, Shingo Ueno, Hirofumi Shiono
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Patent number: 8592348Abstract: A method for producing a chip on which biomolecules are immobilized in an aligned state, comprises (a) producing a substrate 1 on which a plurality of biomolecules 1 of a single type are immobilized in an aligned state, (b) adding reaction reagents for synthesizing biomolecules 2 to microreactors on a microreactor chip comprising the microreactors at positions overlapping with the sequence positions of the biomolecules 1 immobilized on the substrate 1 produced in step (a), (c) closely attaching the microreactor chip to the substrate 1 so that the reaction reagents for synthesizing the biomolecules 2 are allowed to come into contact with the biomolecules 1, so as to synthesize the biomolecules 2 in the microreactors, and (d) superposing the microreactor chip on a substrate 2 after completion of step (c) so as to bind the biomolecules 2 onto the substrate 2; and a chip produced thereby.Type: GrantFiled: November 1, 2007Date of Patent: November 26, 2013Assignee: Japan Science and Technology AgencyInventors: Naoto Nemoto, Takanori Ichiki, Manish Biyani
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Publication number: 20100035769Abstract: It is an object of the present invention to provide a method for producing a biomolecule assay chip using a microreactor technique, and a chip produced by the method.Type: ApplicationFiled: November 1, 2007Publication date: February 11, 2010Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Naoto Nemoto, Takanori Ichiki, Manish Biyani
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Publication number: 20080312103Abstract: The present invention provides a linker preferably used when constructing an mRNA/cDNA-puromycin-protein conjugate used in an in vitro virus method, and an mRNA/cDNA-puromycin-protein conjugate constructed using that linker. More specifically, the present invention provides a linker for ligating mRNA and puromycin or a puromycin-like compound to construct an mRNA/cDNA-puromycin-protein conjugate, the linker comprising a single-stranded RNA as a main backbone, and having, in this main backbone, a solid phase binding site for binding an mRNA-puromycin-protein conjugate to a solid phase site, and a pair of cleavage sites provided at locations surrounding the solid phase binding site; an mRNA-puromycin-protein conjugate constructed using this linker; an mRNA bead or an mRNA chip comprising this conjugate; a protein chip produced from this mRNA chip; and a diagnostic kit using the mRNA bead or the mRNA chip.Type: ApplicationFiled: October 12, 2005Publication date: December 18, 2008Inventors: Naoto Nemoto, Manish Biyani