Abstract: To provide a cell culture device, a cell culture system and a cell culture method capable of suppressing fluctuations in pressure in a culture fluid, and suppressing gas bubbles from flowing into a cell culture section. A cell culture device including: a cell culture section that cultures cells; a storage section that stores a culture fluid; flow paths that connect the cell culture section and the storage section; a fluid delivery device that is provided at the flow paths and that delivers the culture fluid from the storage section to the cell culture section; a pressure equalizing unit that is provided at the flow paths and that suppresses fluctuations in pressure imparted to the culture fluid delivered to the cell culture section; and a pressurization unit that is provided at the flow path at a flow outlet side of the cell culture section and that applies a specific pressure to the culture fluid.

Abstract: A cell culture method is disclosed which includes: a seeding process of singly seeding single cells on a coating layer of a microchannel, the coating layer being present on an inner wall of the microchannel, and the coating layer containing laminin; and a circulation process of circulating a culture fluid in the microchannel where the single cells have been singly seeded in the seeding process.

Abstract: A temperature controlling unit (X1) includes a holder (11) for a liquid receiver (40), a heating block (12) for heating the liquid in the liquid receiver (40), and a cooling block (13) for cooling the liquid in the liquid receiver (40). The holder (11) maintains a first temperature for keeping the temperature of the liquid in the liquid receiver (40) at a lower target temperature. The heating block (12) maintains a second temperature higher than a higher target temperature above the lower target temperature. The cooling block (13) maintains a third temperature lower than the lower target temperature. A temperature controlling method of the present invention includes a heating step for bringing a heating block (12) into contact with the liquid receiver (40) held by the holder (11) and a cooling step for bringing a cooling block (13) into contact with the liquid receiver (40) held by the holder (11).

Abstract: To provide a cell culture device, a cell culture system and a cell culture method capable of suppressing fluctuations in pressure in a culture fluid, and suppressing gas bubbles from flowing into a cell culture section. A cell culture device including: a cell culture section that cultures cells; a storage section that stores a culture fluid; flow paths that connect the cell culture section and the storage section; a fluid delivery device that is provided at the flow paths and that delivers the culture fluid from the storage section to the cell culture section; a pressure equalizing unit that is provided at the flow paths and that suppresses fluctuations in pressure imparted to the culture fluid delivered to the cell culture section; and a pressurization unit that is provided at the flow path at a flow outlet side of the cell culture section and that applies a specific pressure to the culture fluid.

Abstract: The one aspect of the present invention aims to provide a novel cloning method for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), which method is based on culture of dispersed single cells without addition of an apoptotic agent. More specifically, the one aspect of the present invention aims to provide a cloning method for hESCs and hiPSCs based on culture of dispersed single cells utilizing shear stress. The above problem is solved by providing a method for culturing dispersed single pluripotent cells, wherein the dispersed single cells are cultured under laminar flow conditions.

Abstract: A sealing layer covers more surely both of a display region and a peripheral region on a substrate. A dummy structure is formed in the peripheral region of the substrate. The dummy structure contains, for instance, at least one of the materials constituting an organic EL display structure. The dummy structure is located in the peripheral region so that the volume per unit area of the sealing layer in the peripheral region is substantially the same as that in the display region.

Abstract: A sealing layer covers more surely both of a display region and a peripheral region on a substrate. A dummy structure (36) is formed in the peripheral region (15) of the substrate. The dummy structure (36) contains, for instance, at least one of the materials constituting an organic EL display structure (18). The dummy structure (36) is located in the peripheral region so that the volume per unit area of the sealing layer (40) in the peripheral region (15) is substantially the same as that in the display region (13).

Abstract: A temperature controlling unit (X1) includes a holder (11) for a liquid receiver (40), a heating block (12) for heating the liquid in the liquid receiver (40), and a cooling block (13) for cooling the liquid in the liquid receiver (40). The holder (11) maintains a first temperature for keeping the temperature of the liquid in the liquid receiver (40) at a lower target temperature. The heating block (12) maintains a second temperature higher than a higher target temperature above the lower target temperature. The cooling block (13) maintains a third temperature lower than the lower target temperature. A temperature controlling method of the present invention includes a heating step for bringing a heating block (12) into contact with the liquid receiver (40) held by the holder (11) and a cooling step for bringing a cooling block (13) into contact with the liquid receiver (40) held by the holder (11).

Abstract: A sealing layer covers more surely both of a display region and a peripheral region on a substrate. A dummy structure (36) is formed in the peripheral region (15) of the substrate. The dummy structure (36) contains, for instance, at least one of the materials constituting an organic EL display structure (18). The dummy structure (36) is located in the peripheral region so that the volume per unit area of the sealing layer (40) in the peripheral region (15) is substantially the same as that in the display region (13).