Abstract: A method for concentrating and detecting minute amounts of microscopic substances (especially pathogenic viruses) present in aqueous solutions containing biological materials such as saliva, throat wipes, and fecal suspension at three-digit microliter level volumes by adding basic substance, chelating agent, reducing agent, and protein component to PEG solutions. By combining PEG solution with these reagents and highly sensitive detection technology, it has become possible to detect and monitor microscopic substances such as viruses present in large volumes of biological samples and environmental and food materials easily, rapidly, and sensitively. As a result, it is now possible to contribute to the prevention of virus infection in the medical field and/or public and food safety field, etc.

Abstract: The present invention addresses the problem of providing, by a method in which an expensive manufacturing apparatus is not required, an implant material having osteoconductivity superior to that of an implant material containing an aromatic polyether ketone. The present invention pertains to: said method including immersing an aromatic polyether ketone in a strong base solution in the absence of a calcium ion, and immersing an aromatic polyether ketone, which is obtained by the immersing, in a liquid containing a phosphorus-containing compound; and an implant material obtained by said method.

Abstract: Provided is a method suitable for concentrating the viruses present in the environment, food or biomaterials in fields such as inspections for ensuring public or food safety and medical examinations, and reagents used therein. By adding an optimum concentration of polysaccharide such as glycogen to polyethylene glycol (PEG) and optimizing the concentration of a salt to be added, the viruses suspended in an aqueous solution can be easily, rapidly and stably recovered at a high recovery rate.

Abstract: The present invention relates to a method for easily, rapidly and stably concentrating the viruses present in an aqueous solution at a high recovery rate, by adding a PEG solution containing the optimum concentration of polysaccharide and salt, shortening the settling time before centrifugation and relaxing the centrifugation conditions etc., and reagents used therein. The combined use of the method and the reagents for virus concentration with high-sensitive detection techniques and the reaction reagents used therein, enables easy and rapid detection or monitoring of viruses present in the environment, food materials or biomaterials with high sensitivity, which can contribute to the protection of viral infections in the fields of public safety, food safety and medicine.

Abstract: A sheetfed resist removing apparatus having a substrate (111) being a cleaning object placed therein, includes a treatment chamber (101) forming a closed space, and a spray nozzle (102) to spray a cleaning liquid in a form so-called liquid drops over a surface of the substrate (111), in which the treatment chamber (101) forms the closed space containing the substrate (111) such that the placed substrate (111) faces the spray nozzle (102). This structure allows a cleaning liquid to be in the form of liquid drops in consideration of energy reduction, and permits desirable regulation of the temperature of the liquid drops when the liquid drops contact with the resist film in spraying the cleaning liquid over the resist film on the surface of the substrate (111) to thereby remove the resist film, so that secure removal of the resist film can be accomplished.

Abstract: The present invention provides a method for inactivating RNase which generally presents in a sample such as biological sample (especially an excrement sample), or in a sample such as a living body-derived sample (especially an excrement-derived sample) obtained by separation of an RNA-including body therefrom or the like; a method for extracting and detecting RNA from the sample. An RNA extraction method, comprising the steps of: obtaining a mixture under a heating condition, said mixture comprising: a sample comprising an RNA-including body and RNase, and an alkaline treating reagent comprising at least a reducing agent, and having pH of 8.1 or higher, and conducting inactivation of the RNase and extraction of RNA from the RNA-including body by keeping the mixture under the heating condition. An RNA detection method, comprising conducting RNA amplification reaction by mixing a treated sample liquid comprising RNA extracted by the extraction method and an amplification reaction solution.

Abstract: The present invention provides a method for examining a foreign matter derived from a living body in quality control for production of various products in order to rapidly identify an individual from whom a living body-derived material contaminated as a foreign matter in products or facilities involved in production of the products was derived, while securing the secret of information on nucleic acid sequences unique to individuals. The method for examining a foreign matter derived from a living body includes identifying an individual from whom a living body-derived material contaminated as a foreign matter in products or facilities involved in production of the products was derived, on the basis of information on sequences of nucleic acid contained in the living body-derived material.

Abstract: The present invention is a method for synthesis of nucleic acids to amplify an intended nucleic acid in a region in which a GC content is rich, wherein a polyhydric alcohol and/or ammonium sulfate is present in an amplification reaction solution. According to the present invention, it is possible to amplify nucleic acids in a GC rich region efficiently and directly from a sample such as blood containing lots of PCR inhibitory substances without undergoing a process of isolating and purifying the nucleic acid, even though conducting PCR in the GC rich region tends to be difficult using conventional processes even if purified DNA is used.

Abstract: The present invention provides a method for examining a foreign matter derived from a living body in quality control for production of various products in order to rapidly identify an individual from whom a living body-derived material contaminated as a foreign matter in products or facilities involved in production of the products was derived, while securing the secret of information on nucleic acid sequences unique to individuals. The method for examining a foreign matter derived from a living body includes identifying an individual from whom a living body-derived material contaminated as a foreign matter in products or facilities involved in production of the products was derived, on the basis of information on sequences of nucleic acid contained in the living body-derived material.

Abstract: An object of the present invention is to establish a method for amplifying an RNA in a biologically-derived sample by preparing a reaction solution in which a nucleic acid synthesis is not inhibited even in the presence of various biologically-derived impurities and as well as to enable a convenient, rapid and highly sensitive analysis of the RNA in the sample. In a method of the present invention, a reaction solution having a pH higher than that of an ordinarily employed reaction solution and/or containing a polyamine and/or a sulfated polysaccharide.

Abstract: An object of the present invention is to provide a method of treatment that is useful in conducting a nucleic acid synthesis procedure capable of directly amplifying an intended nucleic acid in a living body-derived sample without purification steps.

Abstract: If polyamine is caused to be present in the reacting system for the synthesis of nucleic acids to amplify target genes from a sample, the inhibition of the synthesis due to impurities in the sample can be reduced and the target genes can be effectively synthesized.

Abstract: This invention is directed to a novel method for PCR amplification wherein PCR is carried out at a higher pH than the pH widely used in the art. Specifically, the buffer solution is adjusted to pH 9.0 to 11.0 at 25.degree. C. Using the present invention, DNA amplification can be successfully carried out following a simple pretreatment. In the present invention whole blood is mixed with a hypotonic solution so that a selective lysis of red blood cells takes place. The residual leukocytes are then collected. The leukocytes are mixed with a polymerization agent, primers and other necessary reagents and PCR is carried out. When the PCR solution is placed at a high temperature for DNA denaturation, the leukocytes are lysed so that the leukocyte DNA is released and can access the primers and the other necessary reactions for PCR in the solution.Cell membranes and proteins are present in the PCR reaction solution due to the lack of a protein extractive step during the pretreatment.

Abstract: The present invention provides a method of nucleic acid synthesis capable of directly amplifying a gene of interest in a salivary sample without purifying DNAs from the sample.According to the present invention, in a method of nucleic acid synthesis by mixing a salivary sample itself and a gene amplification reaction solution, and then subjecting them to an amplification reaction, the salivary sample is heat-treated or treated with a saccharide-degrading enzyme before the reaction. For example, a salivary sample was heat-treated at 0-40.degree. C. for 1 hour, subsequently added directly into a PCR reaction solution, and then subjected to a PCR. The result demonstrates that, as shown in FIG. 3, the amount of the PCR product increased according as the heat-treatment temperature was elevated, and as a consequence, the PCR product sufficient to be detected in the electrophoresis was produced in each of all cases using different amounts of saliva, by heat-treating the sample at 30.degree. C. or above.

Abstract: Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

Abstract: Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

Abstract: Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.