Abstract: An intraoral examination method for determining the state of periodontal disease is provided. The method is an intraoral examination method for measuring a signal intensity of a nucleic acid from an oral bacterial group present in an oral sample, calculating an abundance of the bacterial group from a measured value of the signal intensity, and determining the state of periodontal disease using the obtained calculated value as an index, wherein the abundance of the bacterial group shows a correlation between a bacterial load of a bacterial species that increases as a periodontal pocket value increases and a bacterial load of a bacterial species that decreases as a periodontal pocket value increases.

Abstract: A method for simply predicting the degree of inflammation of periodontal tissue such as an inflamed area (PISA or CAPRS value), and a device (e.g., a DNA chip) used for the method are provided. The method is a method for estimating a periodontal pocket inflammation area by detecting bacterial loads of two or more types of bacteria in saliva and using the obtained detection results as indexes, wherein bacteria to be detected include: a bacterium having a positive correlation between the bacterial load of the bacterium and the periodontal pocket inflammation area and a bacterium having a negative correlation between the bacterial load of the bacterium and the periodontal pocket inflammation area.

Abstract: A method for determining absolute amounts of two or more types of target nucleic acids in a sample, including: (a) mixing a sample with a known amount of a control nucleic acid; (b) co-amplifying all target nucleic acids and a specific nucleic acid group in the sample and the control nucleic acid; (c) determining a total amount of the all target nucleic acids and the specific nucleic acid group in the sample based on an indicator of a total amount of the amplified all target nucleic acids and the amplified specific nucleic acid group and an indicator of an amount of the amplified control nucleic acid; and (d) calculating an absolute amount of each target nucleic acid in the sample based on an occupancy of each target nucleic acid in the total of the all target nucleic acids and the specific nucleic acid group.

Abstract: The invention provides: a composition for preventing the drying of gel with which it is possible to prevent the drying of gel more easily than in the past; a gel complex and a DNA chip containing said composite; and a method for producing said composition, composite, and chip. This problem can be overcome by a composition for preventing the drying of gel that includes a polyhydric alcohol and at least one selected from gelatin, collagen, carrageenan, pectin, and agar.

Abstract: Provided is a microarray for detecting mutations in a ?-globin gene, which is capable of detecting a large number of mutations (specimens) conveniently in a short time. A probe group for detecting mutations in a ?-globin gene containing genes having the sequences set forth in SEQ ID NOs:3, 4, 7, 11, 12, 17, 18 and 25 to 66; a microarray having the probe group immobilized thereon; a method for detecting mutations in a ?-globin gene using the microarray; and a kit for ?-globin gene mutation detection using the microarray and primers, are provided.

Abstract: The purpose of this invention is to provide a biochip holder and holding kit that make it possible to efficiently process large numbers of biochips that have detection samples exposed on both sides. This invention provides a biochip holder characterized by having the following: a concavity (24) that accommodates a biochip (10); and support parts (26) that are provided at the edges of said concavity and support the biochip accommodated inside the concavity such that said biochip is substantially horizontal and the underside thereof is above the bottom surface (24a) of the concavity with a gap therebetween.

Abstract: A microarray is disclosed which contains a plurality of gels having different gel concentrations which are carried in respective hollow portions of the microarray, and in which a probe is immobilized in the gels. A method for producing such a microarray is also disclosed.

Abstract: Provided is a microarray for detecting mutations in a ?-globin gene, which is capable of detecting a large number of mutations (specimens) conveniently in a short time. A probe group for detecting mutations in a ?-globin gene containing genes having the sequences set forth in SEQ ID NOs:3, 4, 7, 8, 11, 12, 17, 18 and 25 to 66; a microarray having the probe group immobilized thereon; a method for detecting mutations in a ?-globin gene using the microarray; and a kit for ?-globin gene mutation detection using the microarray and primers, are provided.

Abstract: The present invention provides: a method for detecting a nucleic acid, which efficiently eliminates non-specific detection of nucleic acids other than the nucleic acid as a detection target, so that detection specificity of the nucleic acid as a detection target can be further improved; or the like. This method for detecting a nucleic acid comprises: (a) a step wherein a gel, on which a probe is immobilized, is brought into contact with a reaction solution which contains a nucleic acid that serves as a template for nucleic acid amplification, a primer set for nucleic acid amplification, a nucleotide unit and a DNA extension enzyme; (b) a step wherein the gel and the reaction solution are subjected to a heat cycle for performing a nucleic acid amplification reaction; (c) a step wherein nucleic acid fragments having a specific base length are selected from among the amplified nucleic acid fragments; and (d) a step wherein the selected nucleic acid fragments are detected.