Patents by Inventor Narasimha C. Samboju
Narasimha C. Samboju has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9187755Abstract: Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.Type: GrantFiled: April 7, 2010Date of Patent: November 17, 2015Assignee: Dow AgroSciences LLCInventors: Jayakumar P. Samuel, Joseph F. Petolino, Narasimha C. Samboju, Steven R. Webb, Kerrm Y. Yau
-
Patent number: 8686222Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using dendrimers, and optionally one or more Cell Penetrating Peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.Type: GrantFiled: October 8, 2010Date of Patent: April 1, 2014Assignee: Dow AgroSciences, LLC.Inventors: Jayakumar P. Samuel, Narasimha C. Samboju, Kerrm Y. Yau, Steven R. Webb, Frank Burroughs
-
Patent number: 8653327Abstract: Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.Type: GrantFiled: July 7, 2011Date of Patent: February 18, 2014Assignee: Agrigenetics, Inc.Inventors: Narasimha C. Samboju, Kerrm Y. Yau, Frank Burroughs, Jayakumar P. Samuel, Steven R. Webb
-
Patent number: 8609420Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.Type: GrantFiled: March 22, 2012Date of Patent: December 17, 2013Assignee: Dow AgroSciences, LLC.Inventors: Jayakumar P. Samuel, Narasimha C. Samboju, Kerrm Y. Yau, Gaofeng Lin, Steven R. Webb, Frank Burroughs
-
Patent number: 8581036Abstract: A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.Type: GrantFiled: March 8, 2011Date of Patent: November 12, 2013Assignee: Dow AgroSciences, LLC.Inventors: Narasimha C. Samboju, Jayakumar P. Samuel, Gaofeng Lin, Steven R. Webb, Frank Burroughs
-
Patent number: 8575424Abstract: Methods for introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall include use of nanoparticles. In some embodiments, the cell comprising a cell wall is a cultured plant cell. Methods include genetically or otherwise modifying plant cells and for treating or preventing disease in any plant, especially crop plants. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with functionalized linear nucleic acid cassette molecules.Type: GrantFiled: July 7, 2011Date of Patent: November 5, 2013Assignee: Dow AgroSciences, LLC.Inventors: Kerrm Y. Yau, Jayakumar P. Samuel, Frank Burroughs, Narasimha C. Samboju, Steven R. Webb
-
Publication number: 20120244569Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.Type: ApplicationFiled: March 22, 2012Publication date: September 27, 2012Applicant: DOW AGROSCIENCES LLCInventors: Jayakumar P. SAMUEL, Narasimha C. SAMBOJU, Kerrm Y. YAU, Gaofeng LIN, Steven R. WEBB, Frank BURROUGHS
-
Patent number: 8206983Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.Type: GrantFiled: August 10, 2011Date of Patent: June 26, 2012Assignee: Dow AgroSciences, LLC.Inventors: P. Samuel Jayakumar, Jeffrey Beringer, Paul R. Schmitzer, Frank Burroughs, Robbi J. Garrison, William M. Ainley, Narasimha C. Samboju
-
Publication number: 20120034697Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.Type: ApplicationFiled: August 10, 2011Publication date: February 9, 2012Applicant: Dow AgroSciences LLCInventors: P. Samuel Jayakumar, Jeffrey Beringer, Paul R. Schmitzer, Frank Burroughs, Robbi J. Garisson, William M. Ainley, Narasimha C. Samboju
-
Publication number: 20120023620Abstract: Methods for introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall include use of nanoparticles. In some embodiments, the cell comprising a cell wall is a cultured plant cell. Methods include genetically or otherwise modifying plant cells and for treating or preventing disease in any plant, especially crop plants. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with functionalized linear nucleic acid cassette molecules.Type: ApplicationFiled: July 7, 2011Publication date: January 26, 2012Applicant: DOW AGROSCIENCES LLCInventors: Kerrm Y. Yau, Jayakumar P. Samuel, Frank Burroughs, Narasimha C. Samboju, Steven R. Webb
-
Publication number: 20120023619Abstract: Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.Type: ApplicationFiled: July 7, 2011Publication date: January 26, 2012Applicant: DOW AGROSCIENCES LLCInventors: Narasimha C. Samboju, Kerrm Y. Yau, Frank Burroughs, Jayakumar P. Samuel, Steven R. Webb
-
Publication number: 20110247100Abstract: A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.Type: ApplicationFiled: March 8, 2011Publication date: October 6, 2011Applicant: DOW AGROSCIENCES LLCInventors: Narasimha C. Samboju, Jayakumar P. Samuel, Gaofeng Lin, Steven R. Webb, Frank Burroughs
-
Patent number: 8012752Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.Type: GrantFiled: December 27, 2007Date of Patent: September 6, 2011Assignee: Dow AgroSciences LLCInventors: P. Samuel Jayakumar, Jeffrey Beringer, Paul R. Schmitzer, Frank Burroughs, Robbi J. Garrison, William M. Ainley, Narasimha C. Samboju
-
Publication number: 20110093982Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using dendrimers, and optionally one or more CPPs. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.Type: ApplicationFiled: October 8, 2010Publication date: April 21, 2011Applicant: DOW AGROSCIENCES LLCInventors: Jayakumar P. Samuel, Narasimha C. Samboju, Kerrm Y. Yau, Steven R. Webb, Frank Burroughs
-
Publication number: 20100311168Abstract: Provided are methods for introducing a sequence specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.Type: ApplicationFiled: April 7, 2010Publication date: December 9, 2010Applicant: DOW AGROSCIENCES LLCInventors: Jayakumar P. Samuel, Joseph F. Petolino, Narasimha C. Samboju, Steven R. Webb, Kerrm Y. Yau
-
Publication number: 20100159598Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.Type: ApplicationFiled: December 27, 2007Publication date: June 24, 2010Applicant: Dow AgroSciences LLCInventors: P. Samuel Jayakumar, Jeffrey Beringer, Paul R. Schmitzer, Frank Burroughs, Robbi J. Garrison, William M. Ainley, Narasimha C. Samboju