Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.

Abstract: A coated article for an electro-mechanical which includes conductors carrying current therewithin, at least one heat convection enabling component disposed in an operable connection with one or more of the conductors, and a coating applied at least partially on the conductors and/or the at least one heat convection enabling component. The coating is a Graphene coating increasing current carrying capacity of the conductors and enhancing operational efficiency of the electro-mechanical device.

Abstract: Systems and methods for using tokenization are provided. In an embodiment, a first computer system receives a notification from a second computer system that user information has changed for a first user account serviced by the second computer system. The notification includes a token corresponding to a second user account serviced by the first computer system. The first user account and the second user account belong to a user. The first computer system searches for the second user account, based on the token, in a database associated with the first computer system. The first computer system updates the second user account to include the user information change and publishes the user information change to other computer systems that subscribe to updates from the first computer system. The published user information change causes the other computer systems to update corresponding user accounts to include the published user information change.

Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.

Abstract: Provided herein are methods and compositions for modifying cells. Provided herein are methods and compositions for modifying an organism of a cell. Provided herein are methods and compositions for introducing polynucleotides and/or polypeptides into a nucleus of a cell.

Abstract: Embodiments provide methods and strictures for purification or volume reduction of a brine by an advanced vacuum distillation process (AVMD) to achieve higher flux by passage of vapors through an AVMD distillation unit. In one example, brine is circulated in a tank. The tank may include one or more membrane pouches that are submerged in the circulating brine or placed above the water level of the hot circulating brine. In other embodiments the membrane pouches are outside of the tank that includes the hot circulating brine but still in communication with it. The circulating brine is heated, allowing creation of water vapor. Using a vacuum, the water vapor is drawn through the membrane, where it may be condensed and subjected to further beneficial use. This process can concentrate to levels to generate crystals or solids, which can be separated and utilized.

Abstract: A portable testing device and method for measuring physical characteristics of a polymeric or elastomeric material is provided. The testing device includes an indenter probe; a drive system for controlling movement of said probe, said drive system comprising a motorized linear slide operatively associated with the probe to advance said probe from a first position to a second position to deform said polymeric or elastomeric material and to facilitate instant or fast retraction of said probe to a predetermined intermediate position between said first and second positions; and a force/displacement measurement system including a first sensor for measuring force at the tip of said probe during contact with said polymeric or elastomeric material and a second sensor for measuring displacement of the probe; and a controller configured to provide control to the force/displacement measurement system and the drive system.

Abstract: Embodiments provide methods and structures for purification or volume reduction of a brine by an advanced vacuum distillation process (AVMD) to achieve higher flux by passage of vapors through an AVMD distillation unit. In one example, brine is circulated in a tank. The tank may include one or more membrane pouches that are submerged in the circulating brine or placed above the water level of the hot circulating brine. In other embodiments the membrane pouches are outside of the tank that includes the hot circulating brine but still in communication with it. The circulating brine is heated, allowing creation of water vapor. Using a vacuum, the water vapor is drawn through the membrane, where it may be condensed and subjected to further beneficial use. This process can concentrate to levels to generate crystals or solids, which can be separated and utilized.

Abstract: A reverse osmosis filter assembly for fluid filtration having a push-activated lock and release mechanism. A push filter design activates a floating key lock upon insertion and extraction, where the filter key may be used simultaneously as a lock and as an identifier for particular filter attributes. The filter base may be situated inline, and in fluid communication, with the raw water ingress, permeate egress, and reject water egress. The reverse osmosis filter housing assembly may be attached to, and removed from, the filter base by a push-actuated release. Upon insertion, the filter key shifts the filter lock longitudinally to receive interlocking segments. Upon extraction, the same axial push shifts the filter lock further to align the interlocking fingers within gaps that allow for easy extraction. The specific key lock design allows a user to identify and match certain filter configurations received by the mechanical support, and reject other filter configurations.

Abstract: A method for fabricating a transistor on a semiconductor wafer includes providing a partial transistor containing a gate stack, extension regions, and source/drain sidewalls. The method also includes performing a source/drain implant of the semiconductor wafer, forming a cap layer over the semiconductor wafer, and performing a source/drain anneal. In addition, the method includes performing a damage implant of the cap layer and removing the cap layer over the semiconductor wafer.

Abstract: An end winding arrangement of a stator of an electro-mechanical device is provided. The end winding arrangement includes at least two stator bars, wherein each stator bar has a linear member and a cell bend member. Each of the at least two stator bars is configured to have a unitary involute member extending from each cell bend member such that the involute member is configured as a single involute section without a lead bend section for reducing vibrations in the electro-mechanical device.

Abstract: A coated article for an electro-mechanical which includes conductors carrying current therewithin, at least one heat convection enabling component disposed in an operable connection with one or more of the conductors, and a coating applied at least partially on the conductors and/or the at least one heat convection enabling component. The coating is a Graphene coating increasing current carrying capacity of the conductors and enhancing operational efficiency of the electro-mechanical device.

Abstract: A manifold for domestic water purification systems having flow paths that can be redirected without having to change the physical structure of the manifold or water purification system—a structure to assist with proper installation and stabilization of specific filter cartridges, a memory reader for processing data relating to the state of the filter cartridges, a hinge system to allow for easier installation/removal of new and replacement filter cartridges, and a flow path structure to assist in adding accessories to the water filtration system without requiring a modification to the manifold structure.

Abstract: Apparatuses, methods, systems, and program products are disclosed for selectively accessing content from a content pool. A method includes receiving content for a content-pool from a content contributor. A method includes determining one or more access settings for the content, the one or more access settings determining how the content is displayed. A method includes providing the content to a presenter according to the one or more access settings.

Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.

Abstract: A manifold for domestic water purification systems having flow paths that can be redirected without having to change the physical structure of the manifold or water purification system—a structure to assist with proper installation of specific filter cartridges, a memory reader for processing data relating to the state of the filter cartridges, a hinge system to allow for easier installation/removal of new and replacement filter cartridges, and a flow path structure to assist in adding accessories to the water filtration system without requiring a modification to the manifold structure.

Abstract: A process for removal of selenium and nitrate from waste water includes both electrochemical and bioprocessing treatment. Embodiments include use of activated walnut shell a growth media for selenium-reducing bacteria.

Abstract: Techniques are provided that produce a risk profile consisting of a risk score and trends of risk scores across devices and sensors in a machine-to-machine (M2M) or Internet of things (IOT) environment. For example, a device is assigned a risk score which is based on baseline factors such as expected network packets between two devices, normal network packets, access to critical devices, authorized access requests from one device to another device, normal communications to a device and the critical ports of a device; access to and conflicts across physical, logical, and operational systems; historical and current usage of these systems, data from public sites, and anomalies from normal behavior patterns. Techniques encompass risk management by computing a risk score in a timely fashion in accordance with an architecture that enables achieving the required scaling necessitated by the huge number of devices in the machine-to-machine (M2M) or Internet of things (IOT) environment.

Abstract: A manifold for domestic water purification systems having flow paths that can be redirected without having to change the physical structure of the manifold or water purification system—a structure to assist with proper installation and stabilization of specific filter cartridges, a memory reader for processing data relating to the state of the filter cartridges, a hinge system to allow for easier installation/removal of new and replacement filter cartridges, and a flow path structure to assist in adding accessories to the water filtration system without requiring a modification to the manifold structure.

Abstract: A reverse osmosis filter assembly for fluid filtration having a push-activated lock and release mechanism. A push filter design activates a floating key lock upon insertion and extraction, where the filter key may be used simultaneously as a lock and as an identifier for particular filter attributes. The filter base may be situated inline, and in fluid communication, with the raw water ingress, permeate egress, and reject water egress. The reverse osmosis filter housing assembly may be attached to, and removed from, the filter base by a push-actuated release. Upon insertion, the filter key shifts the filter lock longitudinally to receive interlocking segments. Upon extraction, the same axial push shifts the filter lock further to align the interlocking fingers within gaps that allow for easy extraction. The specific key lock design allows a user to identify and match certain filter configurations received by the mechanical support, and reject other filter configurations.