Patents by Inventor Nariaki TAKATSU
Nariaki TAKATSU has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11220677Abstract: Provided are: a reverse transcriptase mutant including an amino acid mutation at a position corresponding to position 55 of the amino acid sequence of wild-type reverse transcriptase derived from the Moloney murine leukemia virus, wherein the reverse transcriptase mutant is characterized in that the amino acid mutation is a substitution from threonine to another amino acid, and the other amino acid is selected from the group consisting of amino acids having a nonpolar aliphatic side chain and amino acids having a polar acidic functional group side chain; a nucleic acid that encodes the mutant; a method for producing the mutant and the nucleic acid that encodes the mutant; a method for synthesizing cDNA in which the mutant is used; and a composition and kit including the mutant.Type: GrantFiled: December 13, 2017Date of Patent: January 11, 2022Assignee: TAKARA BIO INC.Inventors: Kazuhiko Ishikawa, Takashi Uemori, Nariaki Takatsu
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Publication number: 20210277367Abstract: Provided are: a reverse transcriptase mutant including an amino acid mutation at a position corresponding to position 55 of the amino acid sequence of wild-type reverse transcriptase derived from the Moloney murine leukemia virus, wherein the reverse transcriptase mutant is characterized in that the amino acid mutation is a substitution from threonine to another amino acid, and the other amino acid is selected from the group consisting of amino acids having a nonpolar aliphatic side chain and amino acids having a polar acidic functional group side chain; a nucleic acid that encodes the mutant; a method for producing the mutant and the nucleic acid that encodes the mutant; a method for synthesizing cDNA in which the mutant is used; and a composition and kit including the mutant.Type: ApplicationFiled: December 13, 2017Publication date: September 9, 2021Applicant: TAKARA BIO INC.Inventors: Kazuhiko ISHIKAWA, Takashi UEMORI, Nariaki TAKATSU
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Patent number: 11046939Abstract: The present invention relates to a Thermus aquaticus (Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.Type: GrantFiled: November 24, 2016Date of Patent: June 29, 2021Assignees: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, TAKARA BIO INC.Inventors: Yoshizumi Ishino, Sonoko Ishino, Takeshi Yamagami, Takashi Uemori, Nariaki Takatsu
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Patent number: 10294465Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: GrantFiled: September 26, 2018Date of Patent: May 21, 2019Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
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Patent number: 10280412Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: GrantFiled: December 12, 2018Date of Patent: May 7, 2019Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20190100736Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: December 12, 2018Publication date: April 4, 2019Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
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Publication number: 20190085307Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: September 26, 2018Publication date: March 21, 2019Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
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Publication number: 20190055527Abstract: The present invention relates to a fusion polypeptide containing, in a direction of from an N-terminal side to a C-terminal side, one or more peptides which bind to a PCNA, and a polypeptide having a DNA polymerase activity; a method for amplifying nucleic acids using the polypeptide; and a composition and a kit, containing the polypeptide. According to the present invention, it is made possible to amplify a long-strand DNA in a short time in amplifying nucleic acids in the presence of PCNA even with a Pol I-type DNA polymerase.Type: ApplicationFiled: November 24, 2016Publication date: February 21, 2019Applicants: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, TAKARA BIO INC.Inventors: Yoshizumi ISHINO, Sonoko ISHINO, Takeshi YAMAGAMI, Takashi UEMORI, Nariaki TAKATSU
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Patent number: 10196618Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: GrantFiled: September 26, 2018Date of Patent: February 5, 2019Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20190017037Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: September 26, 2018Publication date: January 17, 2019Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
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Publication number: 20180346889Abstract: The present invention relates to a Thermus aquaticus (Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.Type: ApplicationFiled: November 24, 2016Publication date: December 6, 2018Applicants: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, TAKARA BIO INC.Inventors: Yoshizumi ISHINO, Sonoko ISHINO, Takeshi YAMAGAMI, Takashi UEMORI, Nariaki TAKATSU
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Patent number: 10131890Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: GrantFiled: March 13, 2014Date of Patent: November 20, 2018Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20160017300Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: March 13, 2014Publication date: January 21, 2016Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI