Patents by Inventor Narisra JONGKAM

Narisra JONGKAM has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • High throughput sequencing of end regions of long linear DNAs
    Patent number: 9938522
    Abstract: This invention relates to linking, amplifying and sequencing of two ends of long linear DNAs. In particular, this invention provides methods for pairing and sequencing VH and VL genes that encode two parts of one immunoglobulin. The method of the present invention can be applied to rapid antibody discovery and engineering.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: April 10, 2018
    Assignee: GENEWIZ, INC.
    Inventors: Shihong Li, Narisra Jongkam, Ruqin Kou, Hairong Duan, Zhenzhen Zhou, Shifang Zhang
  • Methods and compositions for amplification and sequencing of difficult DNA templates
    Patent number: 9441271
    Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
    Type: Grant
    Filed: June 1, 2015
    Date of Patent: September 13, 2016
    Assignee: GENEWIZ INC., SUZHOU
    Inventors: Shihong Li, Narisra Jongkam, Shifang Zhang, Conrad Leung
  • HIGH THROUGHPUT SEQUENCING OF END REGIONS OF LONG LINEAR DNAs
    Publication number: 20160090590
    Abstract: This invention relates to linking, amplifying and sequencing of two ends of long linear DNAs. In particular, this invention provides methods for pairing and sequencing VH and VL genes that encode two parts of one immunoglobulin. The method of the present invention can be applied to rapid antibody discovery and engineering.
    Type: Application
    Filed: September 25, 2015
    Publication date: March 31, 2016
    Applicant: GENEWIZ, INC.
    Inventors: Shihong Li, Narisra Jongkam, Ruqin Kou, Hairong Duan, Zhenzhen Zhou, Shifang Zhang
  • Methods and compositions for amplification and sequencing of difficult DNA templates
    Patent number: 9150914
    Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: October 6, 2015
    Assignee: Genewiz Inc., Suzhou
    Inventors: Shihong Li, Narisra Jongkam, Shifang Zhang, Conrad Leung
  • Methods and Compositions for Amplification and Sequencing of Difficult DNA Templates
    Publication number: 20150259733
    Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
    Type: Application
    Filed: June 1, 2015
    Publication date: September 17, 2015
    Applicant: GENEWIZ INC., SUZHOU
    Inventors: Shihong LI, Narisra JONGKAM, Shifang ZHANG, Conrad LEUNG
  • Methods and Compositions for Amplification and Sequencing of Difficult DNA Templates
    Publication number: 20130316352
    Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
    Type: Application
    Filed: March 28, 2013
    Publication date: November 28, 2013
    Inventors: Shihong LI, Narisra JONGKAM, Shifang ZHANG, Conrad LEUNG