Patents by Inventor Nat L. Sternberg

Nat L. Sternberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Vitro headful packaging system for cloning DNA fragments as large as 95kb
    Patent number: 5378618
    Abstract: This relates to an in vitro headful packaging system for cloning foreign DNA fragments as large as 95 kb. Also of concern is a method of cloning and controlling amplification of DNA fragments as large as 95 kb in a vector containing a multicopy replicon under control of a lac promoter as well as vectors useful for cloning foreign fragments of DNA as large as 95 kb and lysogens to prepare pac-cleavage proficient extract and head-tail proficient extract.
    Type: Grant
    Filed: September 29, 1992
    Date of Patent: January 3, 1995
    Assignee: E. I. Du Pont de Nemours and Company
    Inventors: Nat L. Sternberg, Brian L. Sauer
  • Positive selection vector for the bacteriophage P1 cloning system
    Patent number: 5300431
    Abstract: Positive selection cassettes are disclosed which contain a lethal gene, a promoter, a repressor sequence overlapping the promoter, and a cloning site between the promoter and the lethal gene. Insertion of a foreign nucleic acid sequence into the cloning site prevents expression of the lethal gene. Expression of the lethal gene under nonrepressed conditions kills a host organism containing a positive selection cassette which does not contain the foreign nucleic acid sequence.
    Type: Grant
    Filed: February 26, 1991
    Date of Patent: April 5, 1994
    Assignee: E. I. Du Pont de Nemours and Company
    Inventors: James C. Pierce, Nat L. Sternberg