Patents by Inventor Natalay Y. Kouprina

Natalay Y. Kouprina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
    Patent number: 9139849
    Abstract: Methods are described for construction of long synthetic arrays of DNA repeats, such as alphoid repeats or other repeat sequences. The methods include concatamerization of DNA into short repeats (for instance using rolling circle amplification or directional in vitro ligation), followed by assembling the short repeats into long arrays by homologous recombination during transformation into microbe cells. These methods can be described generally as Recombinational Amplification of Repeats (RAR). The long arrays are engineered centromere-like regions that allow one to construct mammalian artificial chromosomes with a predefined centromeric region structure. Artificial chromosomes, including human artificial chromosomes with a regulated centromere, and methods of their use are also provided.
    Type: Grant
    Filed: April 7, 2006
    Date of Patent: September 22, 2015
    Assignees: The United States of America as Represented by the Government of the Department of Health and Human Services, The University Court of the University of Edinburgh
    Inventors: Vladimir L. Larionov, William C. Earnshaw, Reto Gassman, Stefanie Kandels-Lewis, Hiroshi Masumoto, Megumi Nakano, Vladimir Noskov, Natalay Y. Kouprina, Carl J. Barrett, Stefano Cardinale
  • RAPID GENERATION OF LONG SYNTHETIC CENTROMERIC TANDEM REPEATS FOR MAMMALIAN ARTIFICIAL CHROMOSOME FORMATION
    Publication number: 20090136924
    Abstract: Methods are described for construction of long synthetic arrays of DNA repeats, such as alphoid repeats or other repeat sequences. The methods include concatamerization of DNA into short repeats (for instance using rolling circle amplification or directional in vitro ligation), followed by assembling the short repeats into long arrays by homologous recombination during transformation into microbe cells. These methods can be described generally as Recombinational Amplification of Repeats (RAR). The long arrays are engineered centromere-like regions that allow one to construct mammalian artificial chromosomes with a predefined centromeric region structure.
    Type: Application
    Filed: April 8, 2006
    Publication date: May 28, 2009
    Inventors: Vladimir L. Larionov, William C. Earnshaw, Reto Gassman, Stefanie Kandels-Lewis, Hiroshi Masumoto, Megumi Nakano, Stefano Cardinale, Carl J Barrett, Vladimir Noskov, Natalay Y. Kouprina
  • Transformation-associated recombination cloning
    Patent number: 6391642
    Abstract: The invention is directed to a method of making a yeast artificial chromosome (YAC) comprising introducing into yeast cells a population of nucleic acids and a vector, wherein the vector comprises a yeast centromere, a selectable marker, a yeast telomere, and a sequence which can recombine with a region of a nucleic acid within the population of nucleic acids, whereby in vivo recombination makes the YAC. The invention is also directed to a method of making a YAC using two vectors and a method of making a circular YAC. The invention is also directed toward methods of making YACs with a selected nucleic acid insert from a mixed population of nucleic acids using transformation-associated recombination.
    Type: Grant
    Filed: April 14, 1998
    Date of Patent: May 21, 2002
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Michael A. Resnick, Vladimir L. Larionov, Natalay Y. Kouprina, Edward L. Perkins