Abstract: Described herein are methods and devices for ligating nucleic acids or nucleic acid fragments as well as methods for transforming or transfecting cells with exogenous DNA. The methods and devices generally involve exposing the nucleic acids, nucleic acid fragments, and/or cells to an electromagnetic field, for example, a mini-current electromagnetic field, while performing the steps of ligation or transformation. The methods and devices provide numerous advantages over conventional ligation and transformation techniques and systems such as reduced reaction times, no heating requirements, and reduced amounts of reagents. Finally, as shown in the examples below, the methods and devices described herein are more effective compared to conventional ligation and transformation techniques and systems.

Abstract: Described herein are methods and devices for ligating nucleic acids or nucleic acid fragments as well as methods for transforming or transfecting cells with exogenous DNA. The methods and devices generally involve exposing the nucleic acids, nucleic acid fragments, and/or cells to an electromagnetic field, for example, a mini-current electromagnetic field, while performing the steps of ligation or transformation. The methods and devices provide numerous advantages over conventional ligation and transformation techniques and systems such as reduced reaction times, no heating requirements, and reduced amounts of reagents. Finally, as shown in the examples below, the methods and devices described herein are more effective compared to conventional ligation and transformation techniques and systems.

Abstract: Methods and devices for amplifying nucleic acids generally involve exposing the nucleic acid to an electromagnetic field, for example a mini-current magnetic field, while performing the steps of PCR. The PCR methods and devices provide numerous advantages over conventional PCR techniques and systems such as reduced reaction times, no heating requirements, and reduced amounts of reagents (e.g., optional use polymerases and primers). Additionally, the PCR methods and devices require significantly shorter reaction times (e.g., less than one hour) compared to conventional PCR techniques and systems (minimum 2 hours). Finally, as shown in the Examples, the PCR methods and devices amplify significantly more DNA compared to conventional PCR techniques and systems. Accordingly, the PCR methods and devices provide a more efficient and cost-effective way to perform PCR when compared to conventional PCR techniques and systems.

Abstract: Methods and devices for amplifying nucleic acids generally involve exposing the nucleic acid to an electromagnetic field, for example a mini-current magnetic field, while performing the steps of PCR. The PCR methods and devices provide numerous advantages over conventional PCR techniques and systems such as reduced reaction times, no heating requirements, and reduced amounts of reagents (e.g., optional use polymerases and primers). Additionally, the PCR methods and devices require significantly shorter reaction times (e.g., less than one hour) compared to conventional PCR techniques and systems (minimum 2 hours). Finally, as shown in the Examples, the PCR methods and devices amplify significantly more DNA compared to conventional PCR techniques and systems. Accordingly, the PCR methods and devices provide a more efficient and cost-effective way to perform PCR when compared to conventional PCR techniques and systems.