Abstract: Among the various aspects of the present disclosure relates to methods and compositions useful in convert inflammatory dietary fatty acids (omega 6) to anti-inflammatory fatty acids (omega 3). Specifically, the present disclosure provides methods and compositions for increasing n-3 desaturase expression or activity in a subject. The disclosure provides various compositions for providing increased n-3 desaturase expression or activity including a variety of viral vector and engineered microorganisms The disclosure provides, in part, methods for reducing local and/or systemic inflammation, improving wound healing, preventing premature cellular enescence, treating or preventing a disease, disorder, or condition related to inflammation or obesity and treating or preventing arthritis.