Patents by Inventor Natalia Viktorovna Stoynova

Natalia Viktorovna Stoynova has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for producing an L-amino acid using a bacterium of the family Enterobacteriaceae having an attenuated expression of a gshA gene
    Patent number: 11746345
    Abstract: The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.
    Type: Grant
    Filed: May 26, 2016
    Date of Patent: September 5, 2023
    Assignee: AJINOMOTO CO., INC.
    Inventors: Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova
  • METHOD FOR PRODUCING 2-METHYL-BUTYRIC ACID BY BACTERIAL FERMENTATION
    Publication number: 20220213515
    Abstract: The present invention provides a method for producing 2-methyl-butyric acid by fermentation using a bacterium belonging to the order Enterobacterales which has been modified to attenuate expression of a tyrB gene encoding a protein having tyrosine aminotransferase activity. The method also allows for production of a byproduct substance of 2-methyl-butyric acid during fermentation of the Enterobacterales bacterium having 2-methyl-butyric acid-producing ability.
    Type: Application
    Filed: March 24, 2022
    Publication date: July 7, 2022
    Applicant: AJINOMOTO CO., INC.
    Inventors: Natalia Viktorovna Stoynova, Elena Viktorovna Sycheva, Anna Alexandrovna Ublinskaya, Valery Vasilievich Samsonov, Alexandr Dmitrievich Kivero
  • Method of Producing L-Amino Acids
    Publication number: 20220010343
    Abstract: A method is described for producing an L-amino acid including the steps of cultivating in a culture medium an L-amino acid-producing bacterium belonging to the family Enterobacteriaceae to produce and accumulate the L-amino acid in the culture medium, cells of the bacterium, or both, and collecting the L-amino acid from the culture medium, the cells, or both, wherein said bacterium has been modified to overexpress a gene encoding a periplasmic adaptor protein.
    Type: Application
    Filed: September 27, 2021
    Publication date: January 13, 2022
    Applicant: AJINOMOTO CO., INC.
    Inventors: Natalia Viktorovna Stoynova, Olga Nikolaevna Igonina, Evgeniya Aleksandrovna Malykh, Natalya Sergeevna Plekhanova, Irena Alexandrovna Kononova, Ivan Alexandrovich Butov, Viktor Vasilievich Samsonov, Irina Vladimirovna Biryukova, Irina Segreevna Shmagina
  • AGRICULTURAL OR HORTICULTURAL COMPOSITION CONTAINING CULTURE BROTH OF Bacillus BACTERIUM
    Publication number: 20210015107
    Abstract: An agricultural or horticultural composition is provided. A composition containing a culture broth of a Bacillus bacterium having prephenate dehydratase (PD) resistant to feedback inhibition by phenylalanine.
    Type: Application
    Filed: September 29, 2020
    Publication date: January 21, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Yuta Nagasaka, Daisuke Kitazawa, Kenichi Kaida, Atsushi Hayakawa, Daisuke Igarashi, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova, Kseniia Viktorovna Emelina
  • Method for producing L-isoleucine using a bacterium of the family having overexpressed the cycA gene
    Patent number: 9896704
    Abstract: The present invention provides a method for producing L-isoleucine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress the cycA gene. The method also allows reducing the amount of byproduct amino acids that are produced during fementative production of L-isoleucine using Enterobacteriaceae bacterium having an L-isoleucine-producing ability.
    Type: Grant
    Filed: April 13, 2016
    Date of Patent: February 20, 2018
    Assignee: AJINOMOTO CO., INC.
    Inventors: Christina Dmitrievna Hook, Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova, Tatiyana Sergeevna Sidorova
  • Method for producing an L-amino acid using a bacterium of the family Enterobacteriaceae having attenuated expression of a phosphate transporter-encoding gene
    Patent number: 9873898
    Abstract: The present invention provides a method for producing an L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae. The bacterium can belong to the genus Escherichia, which has been modified to attenuate expression of a phosphate transporter-encoding gene, such as the pitA gene or pitB gene.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: January 23, 2018
    Assignee: AJINOMOTO CO., INC.
    Inventors: Tatyana Mikhailovna Kuvaeva, Evgeniya Aleksandrovna Polyakova, Natalia Viktorovna Stoynova
  • Auto-indicible expression system, and the use thereof for producing useful metabolites using a bacterium of the family
    Patent number: 9574216
    Abstract: The present invention describes a method for producing a useful metabolite using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain a gene(s) expression system including elements of the LysR-type protein-regulated transcriptional machinery modified in such a way that auto-inducible positive feedback regulation of the system is mediated by a coinducer. The method is suitable for producing branched-chain L-amino acids, particularly L-valine, L-isoleucine and L-leucine; and D-pantothenic acid.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: February 21, 2017
    Assignee: AJINOMOTO CO., INC.
    Inventors: Elena Viktorovna Sycheva, Valery Vasilievich Samsonov, Ekaterina Alekseevna Savrasova, Natalia Sergeevna Eremina, Natalia Vladimirovna Geraskina, Natalia Viktorovna Stoynova
  • METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING AN ATTENUATED EXPRESSION OF A gshA GENE
    Publication number: 20160348089
    Abstract: The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.
    Type: Application
    Filed: May 26, 2016
    Publication date: December 1, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova
  • Method for Producing an L-Amino Acid Using a Bacterium of the Family Enterobacteriaceae Having Overexpressed the yajL Gene
    Publication number: 20160340681
    Abstract: The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.
    Type: Application
    Filed: August 10, 2016
    Publication date: November 24, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova, Vera Georgievna Doroshenko
  • Method for producing an L-amino acid using a bacterium of the family enterobacteriaceae having overexpressed the yajL gene
    Patent number: 9493777
    Abstract: The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.
    Type: Grant
    Filed: August 10, 2016
    Date of Patent: November 15, 2016
    Assignee: AJINOMOTO CO., INC.
    Inventors: Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova, Vera Georgievna Doroshenko
  • Method for Producing L-Isoleucine Using a Bacterium of the Family Enterobacteriaceae Having Overexpressed the cycA Gene
    Publication number: 20160312254
    Abstract: The present invention provides a method for producing L-isoleucine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress the cycA gene. The method also allows reducing the amount of byproduct amino acids that are produced during fementative production of L-isoleucine using Enterobacteriaceae bacterium having an L-isoleucine-producing ability.
    Type: Application
    Filed: April 13, 2016
    Publication date: October 27, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Christina Dmitrievna Hook, Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova, Tatiyana Sergeevna Sidorova
  • Method for Producing an L-Amino Acid Using a Bacterium of the Family Enterobacteriaceae Having Attenuated Expression of a Phosphate Transporter-Encoding Gene
    Publication number: 20160201100
    Abstract: The present invention provides a method for producing an L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae. The bacterium can belong to the genus Escherichia, which has been modified to attenuate expression of a phosphate transporter-encoding gene, such as the pitA gene or pitB gene.
    Type: Application
    Filed: March 28, 2016
    Publication date: July 14, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Tatyana Mikhailovna Kuvaeva, Evgeniya Aleksandrovna Polyakova, Natalia Viktorovna Stoynova
  • Mutant acetolactate synthase and a method for producing branched-chain L-amino acids
    Patent number: 9279137
    Abstract: A mutant bacterial acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine is described. Also described is a method for producing branched-chain L-amino acids using a bacterium from the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by the use of the acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine. This acetolactate synthase contains a mutant small subunit encoded by the mutant ilvN gene.
    Type: Grant
    Filed: July 17, 2014
    Date of Patent: March 8, 2016
    Assignee: AJINOMOTO CO., INC.
    Inventors: Elena Viktorovna Sycheva, Vsevolod Aleksandrovich Serebryanyy, Tatyana Abramovna Yampolskaya, Ekaterina Sergeevna Preobrazhenskaya, Natalia Viktorovna Stoynova
  • Method for producing an L-amino acid using a bacterium of the family enterobacteriaceae having attenuated expression of the yjjK gene
    Patent number: 9175319
    Abstract: The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.
    Type: Grant
    Filed: April 23, 2014
    Date of Patent: November 3, 2015
    Assignee: AJINOMOTO CO., INC.
    Inventors: Natalia Viktorovna Stoynova, Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Evgeniya Aleksandrovna Polyakova
  • Auto-Indicible Expression System, and the Use Thereof for Producing Useful Metabolites Using a Bacterium of the Family Enterobacteriaceae
    Publication number: 20150017693
    Abstract: The present invention describes a method for producing a useful metabolite using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain a gene(s) expression system including elements of the LysR-type protein-regulated transcriptional machinery modified in such a way that auto-inducible positive feedback regulation of said system is mediated by a coinducer. The method is suitable for producing branched-chain L-amino acids, particularly L-valine, L-isoleucine and L-leucine; and D-pantothenic acid.
    Type: Application
    Filed: September 29, 2014
    Publication date: January 15, 2015
    Applicant: AJINOMOTO CO., INC.
    Inventors: Elena Viktorovna Sycheva, Valery Vasilievich Samsonov, Ekaterina Alekseevna Savrasova, Natalia Sergeevna Eremina, Natalia Vladimirovna Geraskina, Natalia Viktorovna Stoynova
  • Mutant Acetolactate Synthase and a Method for Producing Branched-Chain L-Amino Acids
    Publication number: 20140335574
    Abstract: A mutant bacterial acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine is described. Also described is a method for producing branched-chain L-amino acids using a bacterium from the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by the use of the acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine. This acetolactate synthase contains a mutant small subunit encoded by the mutant ilvN gene.
    Type: Application
    Filed: July 17, 2014
    Publication date: November 13, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Elena Viktorovna Sycheva, Vsevolod Aleksandrovich Serebryanyy, Tatyana Abramovna Yampolskaya, Ekaterina Sergeevna Preobrazhenskaya, Natalia Viktorovna Stoynova
  • METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE FAMILY ENTEROBACTERIACEAE HAVING ATTENUATED EXPRESSION OF THE yjjK GENE
    Publication number: 20140315261
    Abstract: The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.
    Type: Application
    Filed: April 23, 2014
    Publication date: October 23, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Natalia Viktorovna Stoynova, Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Evgeniya Aleksandrovna Polyakova
  • Method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family
    Patent number: 8852897
    Abstract: A method for producing an L-amino acid is described using a bacterium of the Enterobacteriaceae family, wherein the bacterium contains a protein which is able to confer resistance to growth inhibition by L-cysteine.
    Type: Grant
    Filed: May 3, 2013
    Date of Patent: October 7, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Ekaterina Alekseevna Savrasova, Natalia Viktorovna Stoynova, Gen Nonaka, Shunsuke Yamazaki, Kazuhiro Takumi
  • Method for Producing an L-Amino Acid Using a Bacterium of the Enterobacteriaceae Family
    Publication number: 20140147909
    Abstract: The present invention provides a method for producing an L-amino acid from ethanol using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance the expression of the ydbK gene.
    Type: Application
    Filed: February 6, 2014
    Publication date: May 29, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Tatyana Abramovna Yampolskaya, Natalia Viktorovna Stoynova, Natalia Sergeevna Eremina, Irina Borisovna Altman, Leonid Romanovich Ptitsyn
  • Method for producing an L-amino acid using a bacterium of the family
    Patent number: 8679798
    Abstract: The present invention provides a method for producing an L-amino acid from ethanol using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance the expression of the ydbK gene.
    Type: Grant
    Filed: September 2, 2008
    Date of Patent: March 25, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tatyana Abramovna Yampolskaya, Natalia Viktorovna Stoynova, Natalia Sergeevna Eremina, Irina Borisovna Altman, Leonid Romanovich Ptitsyn