Abstract: Systems and methods for visualizing an unstained sperm cell are presented, the system comprises a data input utility that receives measured data comprising at least one quantitative phase microscopy image of the unstained sperm cell; a data processing utility comprising an image analyzer module that utilizes characteristic refractive index information of one or more organelles of the sperm cell, to process the at least one quantitative phase microscopy image and generate at least one corresponding gradient image that includes edge enhancement of the one or more organelles, and a virtual staining module that applies one or more predetermined virtual staining functions to the at least one quantitative phase microscopy image and at least one corresponding gradient image, thereby virtually stain at least one of the one or more organelles of the sperm cell and generate virtually stained image data of the sperm cell; and an output utility that utilizes the virtually stained image data of the sperm cell and generate

Abstract: The present invention provides a novel technique for collective tomography (i.e. analyzing many cells at once), without staining, using one 3D model representing the entire cell population, enabling high-throughput tomography. There is provided a tomographic technique for analyzing a sample containing a population of biological cells. In this technique, at least one tomographic projection may come from another cell in the cell population, and each cell in the population may contribute one or more projections to the final 3D refractive-index map.

Abstract: Some embodiments are directed to a technique having an off-axis interferometric geometry that is capable of spatially multiplexing at least six complex wavefronts, while using the same number of camera pixels typically needed for a single off-axis hologram encoding a single complex wavefront. Each of the at least six parallel complex wavefronts is encoded into an off-axis hologram with a different fringe orientation, and all complex wavefronts can be fully reconstructed. This technique is especially useful for highly dynamic samples, as it allows the acquisition of at least six complex wavefronts simultaneously, optimizing the amount of information that can be acquired in a single camera exposure. The off-axis multiplexing holographic system of some embodiments provide an off-axis holography modality that is more camera spatial bandwidth efficient than on-axis holography.

Abstract: The present invention relates to an automatic real-time label-free holography-activated sorting of the cell's technique. The technique provides high-discriminative power on the level of the individual cell. The technique includes rapid automated cell processing during cell visualization and flow, with high discriminative power on the level of the individual cell. The technique may be useful in detection of cancer and to identify different stages of oncogenesis.

Abstract: Some embodiments are directed to a technique having an off-axis interferometric geometry that is capable of spatially multiplexing at least six complex wavefronts, while using the same number of camera pixels typically needed for a single off-axis hologram encoding a single complex wavefront. Each of the at least six parallel complex wavefronts is encoded into an off-axis hologram with a different fringe orientation, and all complex wavefronts can be fully reconstructed. This technique is especially useful for highly dynamic samples, as it allows the acquisition of at least six complex wavefronts simultaneously, optimizing the amount of information that can be acquired in a single camera exposure. The off-axis multiplexing holographic system of some embodiments provide an off-axis holography modality that is more camera spatial bandwidth efficient than on-axis holography.

Abstract: A reconstruction system is presented for reconstructing morphology of biological cells. The system includes data input and output utilities, memory, and a data processor, and is configured for data communication with an image data provider to receive raw image data comprising a sequence of image frames acquired from the cell during a movement of the cell and being indicative of optical path delay (OPD) of light propagation through the biological cell. The data processor is configured and operable to process the raw image data and determine 3D dynamic morphological data of the cell. The data processor includes: a modeling utility; a position recovery module; and an analyzer module.

Abstract: The present invention relates to a novel label-free tomographic interferometry technique implemented inside an environmental chamber (e.g. imaging incubator) that provides rapid capturing of the three-dimensional (3-D) refractive index distribution of biological cells.

Abstract: Systems and methods for visualizing an unstained sperm cell are presented, the system comprises a data input utility that receives measured data comprising at least one quantitative phase microscopy image of the unstained sperm cell; a data processing utility comprising an image analyzer module that utilizes characteristic refractive index information of one or more organelles of the sperm cell, to process the at least one quantitative phase microscopy image and generate at least one corresponding gradient image that includes edge enhancement of the one or more organelles, and a virtual staining module that applies one or more predetermined virtual staining functions to the at least one quantitative phase microscopy image and at least one corresponding gradient image, thereby virtually stain at least one of the one or more organelles of the sperm cell and generate virtually stained image data of the sperm cell; and an output utility that utilizes the virtually stained image data of the sperm cell and generate

Abstract: Methods and systems for use in sperm analysis are described. A method for processing measured data comprising at least interferometric phase data of label-free sperm cell(s), the processing comprising determining topographic optical phase delay map of the label-free sperm, determining at least one physical parameter of the label-free sperm, and generating data indicative of sperm quality for the label-free sperm. A device comprising a flow channel comprising an inlet for receiving fluid containing cells, a selection zone and at least two outlets at said selection zone; and a flow-driving mechanism comprising a flow-driving unit configured and operable to generate flow of said fluid from said inlet towards at least first of said at least two outlets, and a collecting driving unit selectively operating along the direction of a second outlet of said at least two outlets to direct a portion of interest of said fluid towards said second outlet.

Abstract: Methods and systems for evaluating fertility potential of a living sperm cell are presented and involve providing a predetermined classification function correlating between one or more physio spatial parameters of sperm cells and DNA fragmentation distribution in sperm cells, providing data indicative of the one or more physio spatial parameters of the living sperm cell, and applying the classification function to the one or more physio spatial parameters of the living sperm cell to thereby determine a DNA fragmentation level, inside the DNA fragmentation distribution, in the living sperm cell, the DNA fragmentation level being indicative of the fertility potential of the living sperm cell.

Abstract: The present invention relates to a novel label-free tomographic interferometry technique implemented inside an environmental chamber (e.g. imaging incubator) that provides rapid capturing of the three-dimensional (3-D) refractive index distribution of biological cells.

Abstract: The present invention relates to a new interferometric module which may be implemented as holographic/interferometric portable optical setups that are based on the interferometry with multiple wavelengths acquired simultaneously, without changing the imaging parameters, such as the magnification and the resolution, and recording the quantitative complex wave front (i.e. amplitude and phase) imaging in several wavelengths simultaneously. This may be used for multiple-wavelength phase unwrapping, for recording higher or optically thicker samples or for spectroscopic holography, without loss of camera frame rate due to wavelength scanning.

Abstract: Techniques for extracting phase data of off-axis interferogram images are disclosed. At least one sample-related interferogram image associated with a sample is spectrally decomposed to obtain a set of frequency components thereof, and a portion of the set of frequency components is used to generate at least one complex image having a reduced size being smaller in size than the sample-related interferogram image and being indicative of the phase data of the at least one sample-related interferogram image. The reduced size complex image is then used to generate a phase image of the least one sample-related interferogram image.

Abstract: The present invention relates to a new interferometric module which may be implemented as holographic/interferometric portable optical setups that are based on the interferometry with multiple wavelengths acquired simultaneously, without changing the imaging parameters, such as the magnification and the resolution, and recording the quantitative complex wave front (i.e. amplitude and phase) imaging in several wavelengths simultaneously. This may be used for multiple-wavelength phase unwrapping, for recording higher or optically thicker samples or for spectroscopic holography, without loss of camera frame rate due to wavelength scanning.

Abstract: The present invention provides a novel simple, portable, compact and inexpensive approach for interferometric optical thickness measurements that can be easily incorporated into an existing microscope (or other imaging systems) with existing cameras. According to the invention, the interferometric device provides a substantially stable, easy to align common path interferometric geometry, while eliminating a need for controllably changing the optical path of the beam. To this end, the inexpensive and easy to align interferometric device of the invention is configured such that it applies the principles of the interferometric measurements to a sample beam only, being a single input into the interferometric device.

Abstract: The present invention provides a sample inspection and quantitative imaging system and method for performing off-axis interferometric imaging while enabling to record off-axis holograms in an extended field of view (FOV) than possible using a given camera and imaging setup, and thus to enlarge (e.g. double, triple, or even more than this) the interferometric FOV, without changing the imaging parameters, such as the magnification and the resolution.

Abstract: Methods and systems for use in sperm analysis are described. A method for processing measured data comprising at least interferometric phase data of label-free sperm cell(s), the processing comprising determining topographic optical phase delay map of the label-free sperm, determining at least one physical parameter of the label-free sperm, and generating data indicative of sperm quality for the label-free sperm. A device comprising a flow channel comprising an inlet for receiving fluid containing cells, a selection zone and at least two outlets at said selection zone; and a flow-driving mechanism comprising a flow-driving unit configured and operable to generate flow of said fluid from said inlet towards at least first of said at least two outlets, and a collecting driving unit selectively operating along the direction of a second outlet of said at least two outlets to direct a portion of interest of said fluid towards said second outlet.

Abstract: The present invention provides a novel simple, portable, compact and inexpensive approach for interferometric optical thickness measurements that can be easily incorporated into an existing microscope (or other imaging systems) with existing cameras. According to the invention, the interferometric device provides a substantially stable, easy to align common path interferometric geometry, while eliminating a need for controllably changing the optical path of the beam. To this end, the inexpensive and easy to align interferometric device of the invention is configured such that it applies the principles of the interferometric measurements to a sample beam only, being a single input into the interferometric device.

Abstract: The present invention provides a novel simple, portable, compact and inexpensive approach for interferometric optical thickness measurements that can be easily incorporated into an existing microscope (or other imaging systems) with existing cameras. According to the invention, the interferometric device provides a substantially stable, easy to align common path interferometric geometry, while eliminating a need for controllably changing the optical path of the beam. To this end, the inexpensive and easy to align interferometric device of the invention is configured such that it applies the principles of the interferometric measurements to a sample beam only, being a single input into the interferometric device.

Abstract: The present invention provides a sample inspection and quantitative imaging system and method for performing off-axis interferometric imaging while enabling to record off-axis holograms in an extended field of view (FOV) than possible using a given camera and imaging setup, and thus to enlarge (e.g. double, triple, or even more than this) the interferometric FOV, without changing the imaging parameters, such as the magnification and the resolution.