Patents by Inventor Natasha Popovich

Natasha Popovich has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210215607
    Abstract: Provided herein are cartridges, packaged devices, and systems for improved nucleic acid sequencing. The cartridges, devices, and systems include a highly multiplexed optical chip comprising a plurality of nanoscale reaction regions that is configured to perform and report nucleic acid sequencing reactions. The chips are, in some embodiments, packaged for use in analytical nucleic acid sequencing reactions. The chips may be attached to a printed circuit board, may be surrounded by a protective enclosure, may include a flow cell, and may include optical features to minimize or block photobleaching of the sequencing reagents and background fluorescent signals. Also provided are analytical systems for nucleic acid sequencing that comprise the disclosed cartridges and packaged devices.
    Type: Application
    Filed: January 14, 2021
    Publication date: July 15, 2021
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Russell BERMAN, Jaime Juan BENITEZ-MARZAN, Natasha POPOVICH, Aaron RULISON, Ravi SAXENA
  • Publication number: 20210207190
    Abstract: The present disclosure provides improved methods for isolating asymmetrically-primed and/or asymmetrically-tagged nucleic acid complexes that find use in downstream analytical analyses, including sequence analysis. Compositions comprising such complexes and kits and systems for generating such complexes are also provided.
    Type: Application
    Filed: January 15, 2021
    Publication date: July 8, 2021
    Inventors: Lei Sun, Sassan Sheikholeslami, Natasha Popovich, David Christopher Scherer
  • Publication number: 20210069664
    Abstract: Methods, compositions, and systems for distributing nucleic acids into array regions are provided. The methods, compositions, and systems utilize nucleic acid condensing agents to increase efficiency of distribution of the nucleic acids into the array regions. Various methods for facilitating distribution of the nucleic acids to the array regions are provided.
    Type: Application
    Filed: September 15, 2020
    Publication date: March 11, 2021
    Inventors: Lei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula
  • Patent number: 10920268
    Abstract: The present disclosure provides improved methods for isolating asymmetrically-primed and/or asymmetrically-tagged nucleic acid complexes that find use in downstream analytical analyses, including sequence analysis. Compositions comprising such complexes and kits and systems for generating such complexes are also provided.
    Type: Grant
    Filed: July 16, 2018
    Date of Patent: February 16, 2021
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Lei Sun, Sassan Sheikholeslami, Natasha Popovich, David Christopher Scherer
  • Patent number: 10888832
    Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.
    Type: Grant
    Filed: November 21, 2019
    Date of Patent: January 12, 2021
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Lei Sun, Natasha Popovich, Gene Shen, Thang Pham, Stephen Turner
  • Publication number: 20200385802
    Abstract: The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.
    Type: Application
    Filed: June 23, 2020
    Publication date: December 10, 2020
    Inventors: Sassan Sheikholeslami, Michael Hunkapiller, Natasha Popovich, Lei Sun, Erik Miller, Satwik Kamtekar
  • Patent number: 10814299
    Abstract: Methods, compositions, and systems for distributing nucleic acids into array regions are provided. The methods, compositions, and systems utilize nucleic acid condensing agents to increase efficiency of distribution of the nucleic acids into the array regions. Various methods for facilitating distribution of the nucleic acids to the array regions are provided.
    Type: Grant
    Filed: November 17, 2016
    Date of Patent: October 27, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Lei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula
  • Patent number: 10731211
    Abstract: The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.
    Type: Grant
    Filed: November 17, 2016
    Date of Patent: August 4, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Sassan Sheikholeslami, Michael Hunkapiller, Natasha Popovich, Lei Sun, Erik Miller, Satwik Kamtekar
  • Publication number: 20200224258
    Abstract: Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.
    Type: Application
    Filed: December 9, 2019
    Publication date: July 16, 2020
    Inventors: Gene Shen, Natasha Popovich, Erik Miller, Satwik Kamtekar, Keith Bjornson, Jeremiah Hanes, Stephen Yue, Lubomir Sebo, Zhong Wang
  • Publication number: 20200164335
    Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.
    Type: Application
    Filed: November 21, 2019
    Publication date: May 28, 2020
    Inventors: Lei Sun, Natasha Popovich, Gene Shen, Thang Pham, Stephen Turner
  • Patent number: 10544449
    Abstract: Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.
    Type: Grant
    Filed: January 6, 2017
    Date of Patent: January 28, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Gene Shen, Natasha Popovich, Erik Miller, Satwik Kamtekar, Keith Bjornson, Jeremiah Hanes, Stephen Yue, Lubomir Sebo, Zhong Wang
  • Patent number: 10525438
    Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.
    Type: Grant
    Filed: April 3, 2019
    Date of Patent: January 7, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Lei Sun, Natasha Popovich, Gene Shen, Thang Pham, Stephen Turner
  • Publication number: 20200002765
    Abstract: The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in loading of polymerase enzyme complexes into a predetermined number of reaction sites, including nanoscale wells.
    Type: Application
    Filed: June 28, 2019
    Publication date: January 2, 2020
    Inventors: Jaime Juan Benitez-Marzan, Natasha Popovich, Lei Sun, Satwik Kamtekar, Keith Bjornson, Jeremiah Hanes, Karl Voss, Erik Miller, Thomas Linsky, Leewin Chern
  • Publication number: 20190299187
    Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.
    Type: Application
    Filed: April 3, 2019
    Publication date: October 3, 2019
    Inventors: Lei Sun, Natasha Popovich, Gene Shen, Thang Pham, Stephen Turner
  • Patent number: 10300452
    Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: May 28, 2019
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Lei Sun, Natasha Popovich, Gene Shen, Thang Pham, Stephen Turner
  • Publication number: 20190024140
    Abstract: The present disclosure provides improved methods for isolating asymmetrically-primed and/or asymmetrically-tagged nucleic acid complexes that find use in downstream analytical analyses, including sequence analysis. Compositions comprising such complexes and kits and systems for generating such complexes are also provided.
    Type: Application
    Filed: July 16, 2018
    Publication date: January 24, 2019
    Inventors: Lei Sun, Sassan Sheikholeslami, Natasha Popovich, David Christopher Scherer
  • Publication number: 20170184580
    Abstract: Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.
    Type: Application
    Filed: January 6, 2017
    Publication date: June 29, 2017
    Inventors: Gene Shen, Natasha Popovich, Erik Miller, Satwik Kamtekar, Keith Bjornson, Jeremiah Hanes, Stephen Yue, Lubomir Sebo, Zhong Wang
  • Publication number: 20170159119
    Abstract: The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.
    Type: Application
    Filed: November 17, 2016
    Publication date: June 8, 2017
    Inventors: Sassan SHEIKHOLESLAMI, Michael HUNKAPILLER, Natasha POPOVICH, Lei SUN, Erik MILLER, Satwik KAMTEKAR
  • Publication number: 20170136433
    Abstract: Methods, compositions, and systems for distributing nucleic acids into array regions are provided. The methods, compositions, and systems utilize nucleic acid condensing agents to increase efficiency of distribution of the nucleic acids into the array regions. Various methods for facilitating distribution of the nucleic acids to the array regions are provided.
    Type: Application
    Filed: November 17, 2016
    Publication date: May 18, 2017
    Inventors: Lei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula, Yufeng Qian
  • Publication number: 20160310926
    Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.
    Type: Application
    Filed: March 23, 2016
    Publication date: October 27, 2016
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Lei SUN, Natasha Popovich, Gene Shen, Thang Pham, Stephen Turner