Abstract: A fluid valve is provided that includes a first planar substrate having a smooth surface or a surface with features, an elastomer disposed on the first substrate, a second planar substrate disposed on another side of the elastomer, where the second substrate has a smooth surface or features, where the first and second substrate are more rigid than the elastomer, where the first substrate, the second substrate or the elastomer has a fluid channel, where the channel is open when the first or second substrate are in a first thermal state or a first compression state, where the channel is closed or partially closed when the first or second substrate are in a second thermal state or a second compression state, where the second thermal state is a different temperature than the first thermal state, where the second compression state is a different pressure than the first compression state.

Abstract: A fluid valve is provided that includes a first planar substrate having a smooth surface or a surface with features, an elastomer disposed on the first substrate, a second planar substrate disposed on another side of the elastomer, where the second substrate has a smooth surface or features, where the first and second substrate are more rigid than the elastomer, where the first substrate, the second substrate or the elastomer has a fluid channel, where the channel is open when the first or second substrate are in a first thermal state or a first compression state, where the channel is closed or partially closed when the first or second substrate are in a second thermal state or a second compression state, where the second thermal state is a different temperature than the first thermal state, where the second compression state is a different pressure than the first compression state.

Abstract: Devices and methods for insect surveillance are disclosed. In particular, the invention relates to devices for collecting samples of salivary fluid from insects and multiplexed high-throughput methods of screening insect samples for specific insect species, genetically modified strains, and insect-borne pathogens. An attractant is used to induce insects to deposit salivary droplets in a 2-dimensional microfluidic array having media-filled pockets sized to collect saliva from a single insect. The array for sample collection can be integrated with a microfluidic device for high-throughput processing of insect samples. The microfluidic device can be designed to perform multiplexed PCR or immunoassays, for example, for insect genotyping or pathogen detection.