Abstract: The invention includes a device for manufacturing mRNA by a continuous-flow recombinant process. The device comprises a housing; a cassette engaged with the housing, the cassette having a reaction chamber therein that holds an input reaction mixture having a DNA template wherein the manufacturing occurs within the cassette. The device has a thermal assembly providing a source of heat or cooling for the cassette, a pump to engage at least one tube within the cassette to control fluid flow through the cassette during manufacturing, a valve assembly to control a direction of fluid flow through the cassette, and a software application enabling a user to selectively manage operation of the device. The invention includes the cassette and the device as separate sub-combinations. The software application includes a non-transitory computer-readable medium containing computer executable instructions and a coupled computer processor or computer to execute the method of the manufacturing of mRNA.

Abstract: A fluid collection system comprises a sealed reagent container that fits inside a sample container and is unsealed by disengaging a seal comprised in a sample container. The sealed reagent container contains a reagent, such as a nucleic acid preservative. The reagent container also includes a sleeve disposed opposite the sealed opening. The sleeve includes a distal opening for receiving a sample and a proximal opening disposed in the sample container for guiding a sample from the distal opening and through the proximal opening into the sample container. Disengaging the seal releases the reagent into the sample container with the sample, where they mix.