Abstract: Provided herein is a manual safety injector that includes a container assembly, an injector assembly and a plunger assembly. The container assembly includes a container pre-filled with 5 to 10 mL of fluid therein and a needle assembly comprising a double-ended needle. The injector assembly includes an inner container holder, a middle body, and an outer holder in a nested arrangement, with the inner container holder movable within the middle body and the middle body movable within the outer holder. A first actuation of the plunger assembly moves the inner container holder distally within the middle body, to extend a distal end of the needle, and moves the needle assembly to place the needle in fluid communication with the container. An additional actuation of the plunger assembly expels fluid from the container and actuates a biasing member in the injector assembly to retract the needle back into the outer holder.

Abstract: A process for the preparation of a tissue matrix suitable for regenerative repair of tissues, including contacting an isolated connective tissue with an amount of detergent and an amount of disinfectant to significantly reduce at least one of lipids, phospholipids, nucleic acids, major histocompatibility (MHC) antigens, contaminating microorganisms, and endotoxins. The process further provides for less significant reduction in proteoglycan content while retaining the overall structure of the tissue matrix produced. Processing may further include micronizing the tissue matrix. Also, a tissue matrix having a scaffold portion and non-structural portion, collectively structured to promote cellular infiltration, attachment, and proliferation. The tissue matrix may be in the form of a sheet, thick sheet, or micronized. Also, kits including a prepared tissue matrix for use in regenerative repair, and kits for preparing a tissue matrix.