Abstract: In accordance with at least one aspect of this disclosure, a sensing system includes a sensor mat configured to conform to a component having a central axis, a first sensor cluster disposed on or in the sensor mat configured to sense one or more conditions at a first location on the component, and a second sensor cluster disposed on or in the sensor mat configured to sense one or more conditions at a second location circumferentially spaced to the first location. In embodiments, the second location can be diametrically opposed to the first location.

Abstract: Disclosed herein are engineered ADAR systems for gene editing.

Abstract: Described herein are methods for engineering proteins and viruses to reduce their immunogenicity, proteins and viruses made by using said methods, including proteins having Cas9 like activity and viruses having AAV5 like activity.

Abstract: The disclosure provides for screening methodologies using gene fragment overexpression that provide for the identification of peptide sequences which can modulate the functional regions of proteins of interests, and uses thereof. The disclosure further relates to peptide, polypeptide and polynucleotide identified by the methods of the disclosure, compositions containing such peptide, polypeptide and polynucleotides and uses thereof.

Abstract: Described herein are methods of avoiding an immune response in a subject being administered a regimen requiring Cas9 in order to optimize and broaden the application of CRIPSR based therapeutics comprising administering immune orthogonal Cas9. Also described herein are methods to modify a Cas9 protein by swapping highly immunogenic peptides or amino acids with less immunogenic counterparts. These methods are particularly useful to enable the application of Cas9 arsenal for repeat treatments. Further provided are Cas9 proteins modified to reduce immunogenicity.

Abstract: Described herein are methods of avoiding an immune response in a subject being administered a regimen requiring Cas9 in order to optimize and broaden the application of CRIPSR based therapeutics comprising administering immune orthogonal Cas9. Also described herein are methods to modify a Cas9 protein by swapping highly immunogenic peptides or amino acids with less immunogenic counterparts. These methods are particularly useful to enable the application of Cas9 arsenal for repeat treatments. Further provided are Cas9 proteins modified to reduce immunogenicity.

Abstract: An indoor geolocation system for determining a location in three-dimensional space includes a plurality of base stations and a mobile device movable about an indoor environment in three dimensions. The mobile device detects electromagnetic signals in the indoor environment emitted by devices other than the base stations, and generates a signal profile based on the signals. The mobile device transmits the signal profile to one or more of the base stations, which forward the signal profile to a remote server. The system determines a location of the in three-dimensional space of the mobile device by comparing the signal profile to data regarding signal profiles at a plurality of locations in the indoor environment. The data regarding signal profiles in the indoor environment may have been captured by a detection device other than the mobile device at a time prior to the detection of the electromagnetic signals by the mobile device.

Abstract: Embodiments of various aspects described herein are directed to methods, systems, and computer readable media for predicting response of cells in vitro or in vivo (e.g., in a subject) to at least one or more agents (e.g., a library of agents). Methods, systems, and computer readable media described herein generally involve a computational algorithm to predict an expected post-treatment genome-wide expression profile of a cell or subject induced by an agent. The expected post-treatment genome-wide expression can be computed as a function of a pre-treatment genome-wide expression profile of the subject and known effects of the agent on gene expression in cells. Methods, systems, and computer readable media described herein can be used for drug repositioning, to select an appropriate treatment for a diseased subject, and/or to identify responsive subjects for a particular treatment.

Abstract: Methods and apparatus for deodorization of steam from a waste treatment autoclave. A system includes an autoclave for sterilization of waste, the autoclave being fluidly linked or coupled to a bottom portion of a condensate tank to enable flow of steam from the autoclave to the condensate tank, and a deodorization tank containing a liquid deodorant. An upper portion of the condensate tank is fluidly linked or coupled to a bottom portion of the deodorization tank to enable steam/air from the condensate tank to bubble through the liquid deodorant and exit from a vent located on a top of the deodorization tank.

Abstract: Embodiments of various aspects described herein are directed to methods, systems, and kits for identifying a functional or physiological state of a target cell. The inventions described herein are based on a novel approach that combines biochemical expression measurements of a sample (e.g., gene expression data) with mapping of the measurements onto a graphical representation of a plurality of reference points (loci). Each reference point corresponds to a reference sample with a known phenotype and reflects interrelationships between multi-dimensional biochemical expression measurements of the reference samples. By locating the sample relative to reference points on the graphical representation, the physiological or functional state of the sample can be identified. The methods, systems and kits described herein can be used for various applications, including, e.g., but not limited to, determining an effect of a perturbagen on a target cell, molecule screening, and diagnosis and/or treatment of a subject.