Abstract: Mesofluidic separator assemblies are provided that can include at least a pair of supports configured to extend within a pressure differential axis, and at least one level of a plurality of members extending between the pair of supports. Separator assemblies are also provided that can include at least individual members of the plurality defining a plurality of levels extending from a first level configured to have initial contact with the fluid to be separated and a last level configured to have final contact with the fluid to be separated. Assemblies are also provided that can include each member in a set of members being aligned along one axis that is neither parallel nor normal to the pressure differential axis. Assemblies are provided that can include a conduit configured to facilitate the flow of fluid along a pressure differential axis with the conduit defining at least one cross sectional area that is open. Methods for size separating particles within a fluid are provided.