Abstract: Methods and related systems for charging a refrigerant into a climate control system. In an embodiment, the method includes (a) coupling a storage tank to a refrigerant loop of the climate control system through a charging valve; (b) opening and closing the charging valve in a plurality of cycles; and (c) flowing refrigerant from the storage tank to the refrigerant loop through the charging valve when the charging valve is open, during (b). In addition, the method includes (d) determining a detected saturated temperature of the refrigerant within the refrigerant loop after each cycle of the plurality of cycles; and (e) adjusting an amount of time that the charging valve is open during each cycle of the plurality of cycles during (b) as a function of the detected saturated temperature from a previous cycle of the plurality of cycles.

Abstract: This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.

Abstract: Described herein are bioprinters comprising: one or more printer heads, wherein a printer head comprises a means for receiving and holding at least one cartridge, and wherein said cartridge comprises contents selected from one or more of: bio-ink and support material; a means for calibrating the position of at least one cartridge; and a means for dispensing the contents of at least one cartridge. Further described herein are methods for fabricating a tissue construct, comprising: a computer module receiving input of a visual representation of a desired tissue construct; a computer module generating a series of commands, wherein the commands are based on the visual representation and are readable by a bioprinter; a computer module providing the series of commands to a bioprinter; and the bioprinter depositing bio-ink and support material according to the commands to form a construct with a defined geometry.

Abstract: Described herein are bioprinters comprising: one or more printer heads, wherein a printer head comprises a means for receiving and holding at least one cartridge, and wherein said cartridge comprises contents selected from one or more of: bio-ink and support material; a means for calibrating the position of at least one cartridge; and a means for dispensing the contents of at least one cartridge. Further described herein are methods for fabricating a tissue construct, comprising: a computer module receiving input of a visual representation of a desired tissue construct; a computer module generating a series of commands, wherein the commands are based on the visual representation and are readable by a bioprinter; a computer module providing the series of commands to a bioprinter; and the bioprinter depositing bio-ink and support material according to the commands to form a construct with a defined geometry.

Abstract: An electronic gaming machine (EGM) or another type of gaming device may be configured to provide games that involve “WYSIWYG” (what you see is what you get) symbols or “prize on” symbols. The gaming device may be configured to provide the value of all “prize on” symbols that land during a single instance of a game if a prize on trigger symbol, which also may be referred to herein as a blast symbol, lands during the same instance of the game. In some examples, the game may be a base game. According to some examples, a feature does not need to be triggered during the instance of the base game for the value of all prize on symbols that land during the instance of the base game to be awarded. In other examples, the game may be a feature game, such as a “hold and spin” game.

Abstract: A method for providing a persistent feature in an electronic game includes: (A) simulates spinning of a plurality of reels; (B) generating a first spin result; (C) triggering a value feature based on the occurrence of at least one feature symbol appearing on the first reel and at least one feature symbol appearing on the second reel in first spin result, causing the electronic game to award value feature symbols that appear on the other reels; (D) during a second spin, displaying an animation of moving the first and second feature symbols one or more positions of the reel upon; (E) overlaying the moved feature symbols during the second spin of the plurality of reels; and (F) triggering the value feature for the second spin when at least one feature symbol appears on both the first reel and on the second reel.

Abstract: Methods and related systems for operating a climate control system for an indoor space are disclosed. In an embodiment, the method includes establishing a connection between a device and a hub of the climate control system along a first signal pathway, wherein at least a portion of the first signal pathway comprises a short-range radio connection between the device and a first component of the system. In addition, the method includes monitoring one or more parameters of the first signal pathway and one or more parameters of a second signal pathway extending between the device and the hub, wherein at least a portion of the second signal pathway comprises a short-range radio connection between the device and a second component of the system. Further, the method includes re-routing the connection between the portable device and the system hub from the first signal pathway to the second signal pathway.

Abstract: An electro-mechanical displacement exercise apparatus for processing a maximum force potential of an athlete, including a frame a frame including a platform, wherein a section of the platform is connected to a tower, whereby the tower houses control means for manipulating a tension supporting member connected to a force bearing member, a first pull location, whereby the first pull location is positioned on the platform and within the frame and whereby the first pull location is operatively connected with a portion of the tension supporting member, whereby a user may exert a force on the tension supporting member operatively connected to a first actuator; and wherein the first actuator is housed within the tower, and whereby the first actuator includes a motor coupled to a gearbox.

Abstract: A centering apparatus for bi-directionally pivotable hopper doors. The apparatus is coupled between a frame of a hopper car and a bell crank of a hopper door. The apparatus includes a housing with a piston extending from one end. An end of the piston within the housing includes a piston head. A pair of coil springs are disposed within the housing on opposite sides of the piston head and counteract one another to bias the piston head toward the center of the housing and thus bias the hopper door toward a closed state. In a second configuration the apparatus includes a double-acting actuator with a pair of independent piston assemblies. An actuation system coupled to the actuator maintains the actuator and an associated hopper door in a closed position in a normal state and returns the hopper door to the closed position upon a failure in the system.

Abstract: A contact sensor for monitoring breathing of a subject, comprising: a microphone housing defining a first acoustic cavity, a MEMS microphone disposed within the first acoustic cavity; a second acoustic cavity separated from the first acoustic cavity by a cavity wall having a front surface and a rear surface, the second acoustic cavity at least partially defined by the front surface of the cavity wall; an acoustic conduit formed between the first acoustic cavity and the second acoustic cavity through the cavity wall; and a pressure relief vent having a first end terminating at the second acoustic cavity and a second end terminating outside of the second acoustic cavity.

Abstract: Dynamic directory service object creation and certificate management can be performed. In response to discovering a device connected to a network, a corresponding directory service object can be automatically created, and a digital certificate can be automatically acquired and deployed on the device to facilitate authentication. Further, actions can be logged, and notifications generated based on logged actions. Time involved in deploying and configuring directory services is reduced, efficiency is improved, and there is less of a chance for errors associated with manual configuration.

Abstract: Linker-drug compounds and antibody-drug conjugates that bind to human oncology targets are disclosed. The linker-drug compounds and antibody-drug conjugates comprise a splicing modulator drug moiety. The disclosure further relates to methods and compositions for use in the treatment of neoplastic disorders by administering the antibody-drug conjugates provided herein. In an embodiment, the splicing modulator comprises a pladienolide or a pladienolide derivative.

Abstract: Methods and related systems for charging a refrigerant into a climate control system. In an embodiment, the method includes (a) coupling a storage tank to a refrigerant loop of the climate control system through a charging valve; (b) opening and closing the charging valve in a plurality of cycles; and (c) flowing refrigerant from the storage tank to the refrigerant loop through the charging valve when the charging valve is open, during (b). In addition, the method includes (d) determining a detected saturated temperature of the refrigerant within the refrigerant loop after each cycle of the plurality of cycles; and (e) adjusting an amount of time that the charging valve is open during each cycle of the plurality of cycles during (b) as a function of the detected saturated temperature from a previous cycle of the plurality of cycles.

Abstract: Methods and related systems for controlling superheat in a climate control system are disclosed. In an embodiment, the method includes (a) determining a superheat of a refrigerant downstream of a coil of a heat exchanger of the climate control system. In addition, the method includes (b) determining that an expansion valve upstream of the heat exchanger is fully open. Further, the method includes (c) adjusting a speed of air flowing across the coil or a speed of a compressor of the climate control system after (b) based on the determination in (a) to control the superheat of the refrigerant.

Abstract: Methods, apparatus, and systems for performing an ophthalmic diagnostic test are disclosed. In one aspect, a head-wearable device for administering an ophthalmic test to a subject can comprise a head-wearable frame for mounting the device onto the subject's head, and a light seal configured for coupling to the frame so as to isolate at least one eye of the subject from ambient light when the device is worn by the subject.

Abstract: Methods, apparatus, and systems for performing an ophthalmic diagnostic test are disclosed. In one aspect, a head-wearable device for administering an ophthalmic test to a subject can comprise a head-wearable frame for mounting the device onto the subject's head, and a light seal configured for coupling to the frame so as to isolate at least one eye of the subject from ambient light when the device is worn by the subject.

Abstract: Techniques are described for automated operations to determine tree inventory information for an area of land using visual data of overhead image(s), such as by using a trained prediction model specific to an ecological region to which the land area belongs as part of probabilistically determining multiple types of information about trees in that land area, and for subsequently using the determined tree inventory information in one or more manners (e.g., to improve management of trees in that land area). The images may, for example, include spectral satellite images that include at least visible light data for an area of land, and the determined tree inventory information may include information about the trees present on the land area, such as, for example, predictions of particular tree species, quantities of each of the tree species, sizes of the trees, etc.

Abstract: Methods, apparatus, and systems for performing an ophthalmic diagnostic test are disclosed. In one aspect, a head-wearable device for administering an ophthalmic test to a subject can comprise a head-wearable frame for mounting the device onto the subject's head, and a light seal configured for coupling to the frame so as to isolate at least one eye of the subject from ambient light when the device is worn by the subject.

Abstract: Methods, apparatus, and systems for performing an ophthalmic diagnostic test are disclosed. In one aspect, a head-wearable device for administering an ophthalmic test to a subject can comprise a head-wearable frame for mounting the device onto the subject's head, and a light seal configured for coupling to the frame so as to isolate at least one eye of the subject from ambient light when the device is worn by the subject.