Abstract: Provided in some aspects are epigenetic-modifying DNA-targeting systems, such as CRISPR-Cas/guide RNA systems, that bind to or target a target site in a gene or regulatory element thereof in a T cell. In some aspects, the provided epigenetic modifying DNA-targeting systems provided herein modulate a T cell phenotype or activity. In particular, the provided embodiments relate to the transcriptional repression of genes to promote a stem cell-like memory T (TSCM) cell phenotype. In some aspects, also provided are compositions, polynucleotides, vectors, cells, and pluralities and combinations thereof, and methods and uses related to the provided epigenetic-modifying DNA-targeting systems, for example in modulating the phenotype in T cells including in connection with adoptive T cell therapy.

Abstract: Provided in some aspects are epigenetic-modifying DNA-targeting systems, such as CRISPR-Cas/guide RNA systems, that bind to or target a target site in a gene or regulatory element thereof in a T cell. In some aspects, the provided epigenetic modifying DNA-targeting systems provided herein modulate a T cell phenotype or activity. In particular, the provided embodiments relate to the transcriptional activation of genes that promote a stem cell-like memory T (TSCM) cell phenotype. In some aspects, also provided are compositions, polynucleotides, vectors, cells, and pluralities and combinations thereof, and methods and uses related to the provided epigenetic-modifying DNA-targeting systems, for example in modulating the phenotype in T cells including in connection with adoptive T cell therapy.

Abstract: Provided herein are methods, compositions and articles of manufacture for use in connection with cell therapy involving the administration of one or more doses of a therapeutic T cell composition. The cells of the T cell composition express recombinant receptors such as chimeric receptors, e.g. chimeric antigen receptors (CARs) or other transgenic receptors such as T cell receptors (TCRs). Features of the provided embodiments, including the numbers of cells or units of cells administered and/or the potency of administered cells, provide various advantages, such as lower risk of toxicity in subjects administered the T cell compositions.

Abstract: Provided herein are methods, compositions and articles of manufacture for use in connection with cell therapy involving the administration of one or more doses of a therapeutic T cell composition. The cells of the T cell composition express recombinant receptors such as chimeric receptors, e.g. chimeric antigen receptors (CARs) or other transgenic receptors such as T cell receptors (TCRs). Features of the provided embodiments, including the numbers of cells or units of cells administered and/or the potency of administered cells, provide various advantages, such as lower risk of toxicity in subjects administered the T cell compositions.

Abstract: A fry cooker uses a flow of cooking oil or other medium during the cooking operation to strain out or screen particles that cause degradation of the cooking oil or medium. There are several configurations of the fryer that achieve this result. One is a fryer with a first coarse strainer and a second fine sieve or screen. The first screen is used to swiftly screen and separate coarse particles. The second is used as a finer filter to separate finer particles. Either screen may also be adapted to hold a packet for chemically treating and preserving the cooking medium. Another configuration may include a fryer basket that has a straining portion for removing particulates from the cooking medium. The basket may have an upper sealing portion and a lower straining portion.

Abstract: A self-cleaning burner system incorporates an automatic clean cycle when the burner is started up. Cycles may be programmed for once-a-day cleaning cycle or for other desired interval. On start-up, the cycle routes a small amount of fuel to the burner for ignition inside the burner to clean the burner surfaces. The system incorporates an igniter for fast, routine, and safe ignition of the fuel. Thus, small amounts of debris that accumulate on a surface burner are automatically ignited when the burner is started, keeping the burner clean and operating at a high state of efficiency at all times. The self-cleaning burner system may be incorporated into a fryer or other heating appliance for reliable, efficient operation.

Abstract: Improved features for a filter system for a deep fat fryer are described. The fryer includes a movable filter pan disposed below the cooking tank which is fed by gravity when the tank is drained through a downspout. The downspout is curved and rotatable from a down position when it is in use/storage to an up position when it is not in use and a stabilizing clip is mounted on the downspout to secure a separate polishing tube when it is in the down or horizontal position to allow one handle to lift two pipes at the same time for removal of the filter pan. An inline removable filter is provided in the oil return line, and the return line is slidably supported on the side of the filter pan so that it can rise and fall with the debris in the filter pan.