Abstract: The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I): wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

Abstract: The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I): wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

Abstract: Provided are a composition for forming a coating film, a coating film and a substrate having the coating film, which have excellent compatibility with biological substances, as well as a method for producing the same. A composition for forming a coating film having the ability to inhibit adhesion of biological substances, the composition comprising a copolymer and a solvent, wherein the copolymer is obtained by subjecting a monomer mixture to polymerization, wherein the monomer mixture comprises compounds represented by the following formulae (1), (2), and (3): wherein R1 to R6, n, and m are as defined in the Description and Claims, wherein the compound represented by the formula (3) above is contained in a predetermined amount in the monomer mixture, a coating film obtained from the composition, and a substrate having the coating film as well as a method for producing the same.

Abstract: The invention provides a method for producing a copolymer-containing varnish using a monomer having a specific unsaturated bond which does not cause the problem of gelation, a method for producing a composition, a method for producing a coating film, preferably compatible with biomaterials. The method for producing a copolymer-containing varnish comprises copolymerizing a monomer mixture containing compounds of formulas (1) and (2): wherein R1 represents a hydrogen atom or a methyl group, R2 represents an alkylene group having 1 to 6 carbon atoms, n represents an integer of 1 to 30, R11 represents a hydrogen atom or a methyl group, and A1 represents a monovalent organic group having a cationic property, in a solvent for polymerization, in the presence of a radical polymerization initiator, wherein % by mass of the compound of formula (1) in a phosphorus-containing compound contained in the monomer mixture is 70% by mass or more.

Abstract: The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I): wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

Abstract: The invention provides a medium additive, medium composition and a culture method and the like, capable of efficiently culturing cells or tissues in a well dispersed state, and further, permitting cell image analysis of the cells or tissues. The medium additive or medium composition contains agar, which preferably is a low molecular weight agar having a weight average molecular weight of 10,000-60,000. Using same, cells or tissues can be cultured in a well-dispersed state in a medium, and a proliferation promoting effect for the cells or tissues can also be obtained. In addition, the cells can be cultured in any of a floating state and a precipitated state by adjusting the concentration of the aforementioned agar.

Abstract: The invention provides a coating agent for a flow passage which comprises a copolymer containing a recurring unit which contains an organic group of the formula (a) wherein Ua1 and Ua2 are defined herein, and a recurring unit which contains an organic group of the formula (b) wherein Ub1, Ub2, Ub3, and An? are defined herein. The invention also provides a flow passage device having the coating agent for a flow passage on at least part of an inner surface of a flow passage, a platelet-producing flow passage device and a method for manufacturing the same.

Abstract: The invention relates to a cell culture container that inhibits adhesion of cells, a method for manufacturing the same and a method for producing cell aggregates using the same. The cell culture container has a coating film containing a copolymer having a recurring unit which contains a group represented by formula (a) and a recurring unit which contains a group represented by formula (b): wherein Ua1, Ua2, Ub1, Ub2 and Ub3 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and An? represents an anion selected from the group consisting of a halide ion, an inorganic acid ion, a hydroxide ion and an isothiocyanate ion, on at least a part of a surface of the container, and the volume of the container is 20 ?L or less.

Abstract: The present invention provides a copolymer obtainable by polymerizing a monomer mixture which contains at least compounds of the following formulae (A), (B) and (C), wherein Ta, Tb, Tc, Ua1, Ua2, Ub1, Ub2 and Ub3, Qa, Qb and Qc, Ra, Rb and Rc, An? and m are as defined in Specification and Claims, and so on. The copolymer of the present invention can be utilized as an ion complex material excellent in a function of inhibiting adhesion of a biological substance.

Abstract: The present invention provides a cell culture vessel having a surface coated with a copolymer including a repeating unit containing a group represented by the following formula (a), a repeating unit containing a group represented by the following formula (b), and a repeating unit containing a group represented by the following formula (c) (wherein Ua1, Ua2, Ub1, Ub2, Ub3, Rc and An? are as defined in the description and the claims). The invention also provides a method for producing such cell culture vessels, and a method for producing a cell aggregate using the vessel.

Abstract: The invention provides a structural base that includes a coating film on at least a portion of the surface thereof, the coating film having a difference between the maximum film thickness and the minimum film thickness of not more than 1000 ?. A method of the invention produces such structural bases. The coating film includes a copolymer including a repeating unit containing a group represented by formula (a), and a repeating unit containing a group represented by formula (b): wherein Ua1, Ua2, Ub1, Ub2, Ub3 and An? are as defined herein.

Abstract: The present invention is to provide a copolymer obtainable by polymerizing a monomer mixture containing at least compounds of the following formulae (A) and (B) (wherein Ta, Tb, Qa, Qb, Ra, Rb, Ua1, Ua2, Ua3, Ub1, Ub2, Ub3, An? and m are as defined in the present specification and Claims), etc. The copolymer of the present invention can be utilized as an ion complex material excellent in a function of inhibiting adhesion of a biological substance.

Abstract: The invention provides a coating agent for a flow passage which comprises a copolymer containing a recurring unit which contains an organic group of the formula (a) wherein Ua1 and Ua2 are defined herein, and a recurring unit which contains an organic group of the formula (b) wherein Ub1, Ub2, Ub3, and An? are defined herein. The invention also provides a flow passage device having the coating agent for a flow passage on at least part of an inner surface of a flow passage, a platelet-producing flow passage device and a method for manufacturing the same.

Abstract: The invention provides a medium additive, medium composition and a culture method and the like, capable of efficiently culturing cells or tissues in a well dispersed state, and further, permitting cell image analysis of the cells or tissues. The medium additive or medium composition contains agar, which preferably is a low molecular weight agar having a weight average molecular weight of 10,000-60,000. Using same, cells or tissues can be cultured in a well-dispersed state in a medium, and a proliferation promoting effect for the cells or tissues can also be obtained. In addition, the cells can be cultured in any of a floating state and a precipitated state by adjusting the concentration of the aforementioned agar.

Abstract: The present invention provides a copolymer obtainable by polymerizing a monomer mixture which contains at least compounds of the following formulae (A), (B) and (C), wherein Ta, Tb, Tc, Ua1, Ua2, Ub1, Ub2 and Ub3, Qa, Qb and Qc, Ra, Rb and Rc, An? and m are as defined in Specification and Claims, and so on. The copolymer of the present invention can be utilized as an ion complex material excellent in a function of inhibiting adhesion of a biological substance.