Abstract: It is an object of the present invention to provide a detoxified secretome extract of a culture supernatant having high efficacy and high safety, and a method for producing the same. According to the present invention, provided is a detoxified secretome extract of a culture supernatant of mesenchymal stem cells or progenitor cells derived from the mesenchymal stem cells, wherein the detoxified secretome extract comprises at least one of IGFBP, HGF, VEGF, PDGF, EGF, KGF (FGF-7), PDGFR, TGF?, and TGF? secreted by the mesenchymal stem cells or the progenitor cells derived from the mesenchymal stem cells, and wherein lactic acid and ammonia that are metabolic waste products caused by the mesenchymal stem cells or the progenitor cells derived from the mesenchymal stem cells are removed from the detoxified secretome extract.

Abstract: A method for preparing a stromal vascular fraction from an adipose tissue, including a step of treating an adipose tissue with an enzyme solution containing a collagenase and a neutral protease, preferably an enzyme solution free of clostripain and thermolysin, and showing not less than 1 U neutral protease activity with respect to 10,000 U collagenase activity, and recovering cells is provided by the present invention.

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

Abstract: A pen has an ink reservoir configured to store an ink containing decorative particles and a pen tip configured to apply the ink to an ink coating target. The pen tip includes a proximal end to which the ink is supplied from the ink reservoir, a distal end opposite to the proximal end and configured to contact the ink coating target, and a groove opening on the outer circumferential surface of the pen tip and extending from the proximal end to the distal end.

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

Abstract: A pen including an ink reservoir to store an ink; a pen tip which has a proximal end and a distal end opposite to the proximal end and applies an ink to a coating target; an ink guide to guide the ink in the reservoir toward the pen tip; and a holder part which has a through hole through which the pen tip is inserted and holds at least the proximal end of the pen tip while exposing the distal end of the pen tip with the pen tip inserted through the through hole. The holder part includes: an ink holding part capable of holding the ink from the ink guide and formed at least partially around a circumference of the through hole; and a first opening opening on an inner circumferential surface that defines the through hole and communicating with the ink holding part.

Abstract: In an error response circuit an analysis circuit unit analyzes a command transmitted from a first circuit section to a second circuit section, and detects a status of data transfer between the first circuit section and the second circuit section. A response circuit unit generates an error signal in accordance with the detected status of the data transfer in response to the second circuit section changing from a first power consumption state to a second power consumption state in which power consumption is lower than power consumption in the first power consumption state. A switching circuit unit transmits the error signal to the first circuit section in place of a response signal that is responsive to the command and transmitted from the second circuit section to the first circuit section.

Abstract: Provided is a pen including: an ink reservoir configured to store an ink containing decorative particles; and a pen tip configured to apply the ink to an ink coating target. The pen tip includes: a proximal end to which the ink is supplied from the ink reservoir; a distal end opposite to the proximal end and configured to contact the ink coating target; and a groove opening on the outer circumferential surface of the pen tip and extending from the proximal end to the distal end.

Abstract: Provided is a pen including an ink reservoir to store an ink; a pen tip which has a proximal end and a distal end opposite to the proximal end and applies an ink to a coating target; an ink guide to guide the ink in the ink reservoir toward the pen tip; and a holder part which has a through hole through which the pen tip is inserted and holds at least the proximal end of the pen tip while exposing the distal end of the pen tip with the pen tip inserted through the through hole. The holder part includes: an ink holding part capable of holding the ink from the ink guide and formed at least partially around a circumference of the through hole; and a first opening opening on an inner circumferential surface that defines the through hole and communicating with the ink holding part.

Abstract: A cell observation system 1 is a cell observation system 1 for observing a cell held by a microplate 20 having a plurality of wells 21 arranged therein for holding a sample S including the cell and comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, and a data analyzer 50 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21 of the microplate 20, while a leading end of the negative electrode 17a on the well 21 side extends longer than a leading end of the positive electrode 17b on the well 21 side.

Abstract: A cell observation system 1, for measuring fluorescence emitted from a cell held by a microplate 20 having a plurality of wells 21, comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, a position controller 30 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21, a moving image acquisition unit 40 for detecting the fluorescence from the sample S within the wells 21, and a data analyzer 50 for setting a part of a region facing the positive electrode 17b on the well 21 as an analysis region and analyzing an optical intensity in the analysis region so as to acquire analysis information concerning the sample S.

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

Abstract: In an error response circuit an analysis circuit unit analyzes a command transmitted from a first circuit section to a second circuit section, and detects a status of data transfer between the first circuit section and the second circuit section. A response circuit unit generates an error signal in accordance with the detected status of the data transfer in response to the second circuit section changing from a first power consumption state to a second power consumption state in which power consumption is lower than power consumption in the first power consumption state. A switching circuit unit transmits the error signal to the first circuit section in place of a response signal that is responsive to the command and transmitted from the second circuit section to the first circuit section.

Abstract: In an error response circuit an analysis circuit unit analyzes a command transmitted from a first circuit section to a second circuit section, and detects a status of data transfer between the first circuit section and the second circuit section. A response circuit unit generates an error signal in accordance with the detected status of the data transfer in response to the second circuit section changing from a first power consumption state to a second power consumption state in which power consumption is lower than power consumption in the first power consumption state. A switching circuit unit transmits the error signal to the first circuit section in place of a response signal that is responsive to the command and transmitted from the second circuit section to the first circuit section.

Abstract: A cell observation system 1, for measuring fluorescence emitted from a cell held by a microplate 20 having a plurality of wells 21, comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, a position controller 30 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21, a moving image acquisition unit 40 for detecting the fluorescence from the sample S within the wells 21, and a data analyzer 50 for setting a part of a region facing the positive electrode 17b on the well 21 as an analysis region and analyzing an optical intensity in the analysis region so as to acquire analysis information concerning the sample S.

Abstract: A cell observation system 1 is a cell observation system 1 for observing a cell held by a microplate 20 having a plurality of wells 21 arranged therein for holding a sample S including the cell and comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, and a data analyzer 50 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21 of the microplate 20, while a leading end of the negative electrode 17a on the well 21 side extends longer than a leading end of the positive electrode 17b on the well 21 side.

Abstract: A determining method includes obtaining terminal information indicating a first object terminal that is among terminals included among partial circuits and subject to determination of whether the first object terminal is an open terminal; obtaining for each terminal, connection information and first attribute information indicating an attribute of any one among an input terminal and an output terminal; generating, by a computer, for each terminal, second attribute information indicating an attribute opposite to the attribute indicated by the first attribute information; and determining, by the computer, whether a state of the first object terminal indicated by the terminal information becomes a high-impedance state, by simulating on the basis of the connection information and the second attribute information, a state of each terminal when a value of a terminal among the terminals and indicated as an output terminal by the second attribute information, is set at a first specified value.

Abstract: A determining method includes obtaining terminal information indicating a first object terminal that is among terminals included among partial circuits and subject to determination of whether the first object terminal is an open terminal; obtaining for each terminal, connection information and first attribute information indicating an attribute of any one among an input terminal and an output terminal; generating, by a computer, for each terminal, second attribute information indicating an attribute opposite to the attribute indicated by the first attribute information; and determining, by the computer, whether a state of the first object terminal indicated by the terminal information becomes a high-impedance state, by simulating on the basis of the connection information and the second attribute information, a state of each terminal when a value of a terminal among the terminals and indicated as an output terminal by the second attribute information, is set at a first specified value.