Abstract: Provided herein, inter alia, are compositions and methods relating to ?-Hemolysin polypeptide variants for translocation across phospholipid membranes.

Abstract: Provided herein are, inter alia, methods and compositions for linking RNA stem loops. The methods include linking a first RNA stem loop and a second RNA stem loop by way of a preQ1 linking compound.

Abstract: Disclosed herein, inter alia, is a light-controlled bioorthogonal tetrazine ligation.

Abstract: Provided herein, inter alia, are compositions and methods including transmembrane domains comprising a split intein and vesicles including transmembrane domains with a split intein. In embodiments, methods for generating vesicle embedded proteins in vitro without the use of denaturing agents, and their compositions, are provided.

Abstract: Provided herein are, inter alia, methods and compositions for linking RNA stem loops. The methods include linking a first RNA stem loop and a second RNA stem loop by way of a preQ1 linking compound.

Abstract: Provided herein are, inter alia, compositions and methods for modifying a DNA molecule. The methods include contacting a DNA molecule with a transglycosylase enzyme and a Pre-queuosinel (preQi) analog or derivative, wherein a guanine nucleobase within a hairpin structure in the DNA molecule is exchanged for the preQi analog or derivative.

Abstract: Disclosed herein, inter alia, are depalmitoylating compounds, compositions, and methods of use thereof.

Abstract: Provided herein are, inter alia, amphiphilic thiol compounds and methods of using the same for the purpose of depalmitoylating proteins in cellular membranes (e.g., plasma membranes). The compounds provided herein include an amphiphilic tail, which enables them to associate with a cellular membrane and depalmitoylate (cleave native S-palmitoyl groups from) a protein in said membrane by native chemical ligation thereby triggering the protein's release from the plasma membrane. The compounds (amphiphilic thiol compounds of formula (I), (II), (III)) are, inter alia, useful for the treatment of diseases caused or associated with aberrant depalmitoylation of certain proteins (e.g., HRas, EGFR).

Abstract: The present invention provides a method of spatially controlling catalysis of a chemical reaction at a surface of a substrate. With this method, a substrate is provided with two or more independently addressable electrodes at or near the surface of the substrate. Next, a first potential is applied to one or more of the electrodes. This first potential is sufficient to electrochemically generate an active catalyst from an inactive catalyst proximal to any electrode(s) that are at this first potential. Simultaneously, a second potential is applied to all other electrodes. This second potential serves to deactivate any active catalyst that may diffuse from the proximity of the electrode(s) that are at the first potential. This method results in spatially selective activation of catalyst at the electrode(s) that are at the first potential, and hence spatial localization of a chemical reaction catalyzed by the active catalyst.