Abstract: A preparative scale electrical separator, which is matrix-free and may be operated in both a batch mode and a continuous, flow-through mode is provided. It may be scaled according to the solution quantities which are to be processed. An internal cooling system whereby the process fluid is always in close proximity to a chilled surface assures an efficient heat dissipation during the separation and concentration processes and also allows the use of higher voltage gradients and shorter separation times than prior designs of electrophoretic separators. Electroosmotic and convective mixing is reduced by providing internal, compartmentation of sample fractions, focusing is improved and focusing times are decreased. A counter-flow gradient focusing method using the electrophoretic separator allows for separation of cells from human or animal blood and tissues, cells from plants, bacteria and viruses.

Abstract: A preparative scale multi-mode electrical separator, which is matrix-free and may be operated in both a batch mode and a continuous, flow-through mode is provided. It may be scaled according to the solution quantities which are to be processed. An internal cooling system whereby the process fluid is always in close proximity to a chilled surface assures an efficient heat dissipation during the separation and concentration processes and also allows the use of higher voltage gradients and shorter separation times than prior designs of electrophoretic separators. Electroosmotic mixing is reduced by providing internal, compartmentation of sample fractions, focusing is improved and focusing times are decreased. Different electrophoretic methods, including zone electrophoresis, isoelectric focusing, counterflow gradient focusing or electrodialysis, may be used with the separator. It may be applied to the purification of proteins, ions, and microorganisms, and for water purification.