Abstract: Provided herein are method of analyzing genomic and metabolomic data from fungi to identify relationships between biosynthetic gene clusters and mass spectrometric features of metabolites.

Abstract: Provided herein are systems and methods for untargeted screening of metabolite products of biosynthetic gene clusters. In particular, systems and methods are provided for the detection of secondary metabolites and the correlation off such metabolites to the biosynthetic gene clusters responsible for the biosynthesis thereof.

Abstract: Provided herein are systems and methods for untargeted screening of metabolite products of biosynthetic gene clusters. In particular, systems and methods are provided for the detection of secondary metabolites and the correlation off such metabolites to the biosynthetic gene clusters responsible for the biosynthesis thereof.

Abstract: Provided herein are photocrosslinking reagents, crosslinkable proteins displaying photocrosslinking groups, crosslinked protein-protein complexes, and methods of use thereof.

Abstract: Provided herein are photocrosslinking reagents, crosslinkable proteins displaying photocrosslinking groups, crosslinked protein-protein complexes, and methods of use thereof.

Abstract: Provided herein are systems and methods for untargeted screening of metabolite products of biosynthetic gene clusters. In particular, systems and methods are provided for the detection of secondary metabolites and the correlation off such metabolites to the biosynthetic gene clusters responsible for the biosynthesis thereof.

Abstract: Disclosed herein is a system and method for analyzing a specimen containing the proteome by mass spectrometry. The system includes a protein separation module; a matrix processing module; and a mass spectrometer module. The protein separation module, the matrix processing module and the mass spectrometer are in fluid communication with one another.

Abstract: Methods and kits to use in the isolation and identification of crosslinked protein substrate ubiquitin ligase complexes are disclosed. More specifically the methods and kits disclosed herein describe the use of bifunctional thiol-and-amine crosslinkers to covalently bind an endogenous or exogenous HECT-ubiquitin ligase to a downstream protein substrate, preferably in a cell lysate.

Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.

Abstract: Provided herein is technology relating to proteases and proteomics and particularly, but not exclusively, to compositions comprising OmpT, methods of using OmpT, and methods of manufacturing OmpT for proteomics.

Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.

Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic kit facilitating relative quantification and providing tangible evidence for the existence of specific functional groups. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules, such as biological samples. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. Advantageously, the labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.

Abstract: A method of selecting a set of candidate polypeptides for a sample polypeptide that includes a first refining of a collection of candidate polypeptides from differences in mass of fragments of the sample polypeptide produced by mass spectrometry and a second refining of the collection of candidate polypeptides from the absolute mass of the sample polypeptide and the absolute mass of the fragments.

Abstract: A method of preparing a first set of candidate fragments from a sample protein fragment and a protein sequence, comprises selecting a first candidate sequence comprising a terminal amino acid of the protein sequence; generating a further candidate sequence from each candidate sequence, except a last candidate sequence; and including any candidate sequences having a mass which is equal to the mass of the sample protein fragment within a third tolerance, in the first set of candidate fragments.