Patents by Inventor Neil Soice
Neil Soice has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20130225701Abstract: The invention relates to improved methods of grafting polymer extenders onto porous substrates having diffusive pores, such as those used in protein separations, without filing the diffusive pores of the substrate, and restricting diffusion there through. By changing the grafting conditions and/or monomer composition(s) the resulting porous substrates having polymer extenders grafted thereto have increased protein binding capacity and resin selectivity, thereby enhancing the protein separation effectiveness of the substrate. The grafted polymer extenders provide the substrate with significant binding capacity at higher conductivity. The invention also relates to kits, and methods of using and grafting polymer extenders on porous resin substrates having diffusive pores.Type: ApplicationFiled: July 29, 2010Publication date: August 29, 2013Applicant: EMD MILLIPORE CORPORATIONInventors: Neil Soice, Joaquin Umana, Yu Zhang
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Publication number: 20120156757Abstract: The present invention relates, at least in part, to novel and improved chromatography matrices for separating or purifying immunogens, such as, for example, viruses and viral surface proteins, from one or more contaminants in a sample, where the matrix comprises a porous non-polysaccharide solid support comprising a negatively charged, multivalent ion exchange group directly attached to the solid support.Type: ApplicationFiled: December 13, 2011Publication date: June 21, 2012Applicant: Millipore CorporationInventors: Ganapathysubramanian Iyer, Joaquin A. Umana, Neil Soice
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Patent number: 8177537Abstract: The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores.Type: GrantFiled: March 2, 2010Date of Patent: May 15, 2012Assignee: EMD Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Nanying Bian, Brian Gagnon, Umana Joaquin, Neil Soice
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Patent number: 8143067Abstract: Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.Type: GrantFiled: November 3, 2010Date of Patent: March 27, 2012Assignee: EMD Millipore CorporationInventors: Antoni Peters, Philip Goddard, John Charkoudian, Neil Soice, Dave Brewster, Anja Dedeo
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Patent number: 8132676Abstract: Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.Type: GrantFiled: August 6, 2009Date of Patent: March 13, 2012Assignee: EMD Millipore CorporationInventors: Antoni Peters, Philip Goddard, John Charkoudian, Neil Soice, Dave Brewster, Anja Dedeo
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Patent number: 8114611Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: GrantFiled: November 1, 2010Date of Patent: February 14, 2012Assignee: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Chen Wang
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Publication number: 20110313066Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.Type: ApplicationFiled: May 16, 2011Publication date: December 22, 2011Applicant: Millipore CorporationInventors: Jad Jaber, Wilson Moya, James Hamzik, Arezki Boudif, Yu Zhang, Neil Soice, John Charkoudian, Nripen Singh
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Publication number: 20110105730Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: ApplicationFiled: November 1, 2010Publication date: May 5, 2011Applicant: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Chen Wang
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Publication number: 20110065901Abstract: The present invention relates, at least in part, to improved methods of protein purification. In particular, the present invention relates, at least in part, to methods for purifying an Fc region containing protein from a composition comprising the Fc region containing protein and one or more impurities, where the methods eliminate the need for a holding tank and/or a buffer exchange step.Type: ApplicationFiled: August 5, 2010Publication date: March 17, 2011Applicant: MILLIPORE CORPORATIONInventors: Neil Soice, John Dana Hubbard, Yu Zhang, James Hamzik
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Publication number: 20110045493Abstract: Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.Type: ApplicationFiled: November 3, 2010Publication date: February 24, 2011Applicant: MILLIPORE CORPORATIONInventors: Antoni Peters, Philip Goddard, John Charkoudian, Neil Soice, Dave Brewster, Anja Dedeo
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Patent number: 7846682Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: GrantFiled: November 13, 2009Date of Patent: December 7, 2010Assignee: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Chen Wang
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Patent number: 7833723Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: GrantFiled: January 5, 2007Date of Patent: November 16, 2010Assignee: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Chen Wang
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Publication number: 20100221844Abstract: The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.Type: ApplicationFiled: December 18, 2009Publication date: September 2, 2010Applicant: Millipore CorporationInventors: Nanying Bian, Neil Soice, Shari Spector, Kara Levine
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Publication number: 20100174051Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.Type: ApplicationFiled: March 2, 2010Publication date: July 8, 2010Applicant: Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Chen Wang, Nanying Bian, Brian Gagnon, Joaquin A. Umana, Dennis Aquino, Neil Soice, Lyddiatt Andrew
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Patent number: 7678269Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.Type: GrantFiled: September 13, 2006Date of Patent: March 16, 2010Assignee: Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Chen Wang, Nanying Bian, Brian Gagnon, Joaquin A. Umana, Dennis Aquino, Neil Soice, Lyddiatt Andrew
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Publication number: 20100063261Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: ApplicationFiled: November 13, 2009Publication date: March 11, 2010Applicant: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Cheng Wang
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Publication number: 20100044302Abstract: Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.Type: ApplicationFiled: August 6, 2009Publication date: February 25, 2010Inventors: Antoni Peters, Philip Goddard, John Charkoudian, Neil Soice, Dave Brewster, Anja Dedeo
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Publication number: 20090246885Abstract: The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.Type: ApplicationFiled: July 7, 2008Publication date: October 1, 2009Applicant: Millipore CorporationInventors: Nanying Bian, Senthil Ramaswarmy, Neil Soice, Chen Wang, Yuan Wong
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Publication number: 20070256970Abstract: A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.Type: ApplicationFiled: June 21, 2007Publication date: November 8, 2007Applicant: Millipore CorporationInventors: Anthony DiLeo, Justin McCue, Wilson Moya, Igor Quinones-Garcia, Neil Soice, Volkmar Thom, Sarah Yuan
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Publication number: 20070212540Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.Type: ApplicationFiled: September 13, 2006Publication date: September 13, 2007Applicant: Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Chen Wang, Nanying Bian, Brian Gagnon, Joaquin Umana, Dennis Aquino, Neil Soice, Lyddiatt Andrew