Abstract: The invention features Cas9 fusion polypeptides. In one embodiment of the invention, Cas9 is fused to a SNAP tag that enhances Cas9's gene repair function.

Abstract: The invention features Cas9 fusion polypeptides. In one embodiment of the invention, Cas9 is fused to a SNAP tag that enhances Cas9's gene repair function.

Abstract: Disclosed are improved shRNA molecules, termed “organic shRNA” (OshRNA), that incorporate certain structural features that increase the likelihood that the desired guide strand is produced while reducing accumulation of passenger strands that might contribute to off-target effects. Also provided herein are nucleic acids encoding OshRNAs, kits, cells, and transgenic animals comprising such nucleic acids, as well as methods of making and using OshRNAs and/or nucleic acids encoding OshRNAs.

Abstract: Disclosed are improved shRNA molecules, termed “organic shRNA” (OshRNA), that incorporate certain structural features that increase the likelihood that the desired guide strand is produced while reducing accumulation of passenger strands that might contribute to off-target effects. Also provided herein are nucleic acids encoding OshRNAs, kits, cells, and transgenic animals comprising such nucleic acids, as well as methods of making and using OshRNAs and/or nucleic acids encoding OshRNAs.

Abstract: An optical assembly is provided that is capable of high speed operation using a vertical cavity laser such as a vertical cavity surface emitting laser (VCSEL). The optical assembly is configured to have a small form factor and uses an angled surface of an embedded fiber to internally reflect a beam from the VCSEL along the length of the fiber. The angled surface is coated to ensure that a small portion of the beam is transmitted through the fiber out of the angled surface into a monitoring diode. This geometry provides high coupling efficiency and facilitates direct active alignment between the fiber and the VCSEL.

Abstract: An optical assembly is provided that is capable of high speed operation using a vertical cavity laser such as a vertical cavity surface emitting laser (VCSEL). The optical assembly is configured to have a small form factor and uses an angled surface of an embedded fiber to internally reflect a beam from the VCSEL along the length of the fiber. The angled surface is coated to ensure that a small portion of the beam is transmitted through the fiber out of the angled surface into a monitoring diode. This geometry provides high coupling efficiency and facilitates direct active alignment between the fiber and the VCSEL.