Patents by Inventor Neville Espi Sanjana

Neville Espi Sanjana has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220162584
    Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
    Type: Application
    Filed: February 7, 2022
    Publication date: May 26, 2022
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology
    Inventors: Feng Zhang, Bernd Zetsche, Winston YAN, Neville Espi Sanjana, Sara Jones
  • Publication number: 20220135985
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Application
    Filed: October 18, 2021
    Publication date: May 5, 2022
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, The Rockefeller University
    Inventors: Feng Zhang, David Benjamin Turitz Cox, Luciano Marraffini, David Olivier Bikard, Wenyan Jiang, Neville Espi Sanjana
  • Patent number: 11286478
    Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: March 29, 2022
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Feng Zhang, Bernd Zetsche, Winston Yan, Neville Espi Sanjana, Sara Jones
  • Publication number: 20210108196
    Abstract: The present invention generally relates to libraries, compositions, methods, applications, kits and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.
    Type: Application
    Filed: September 21, 2020
    Publication date: April 15, 2021
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology
    Inventors: Feng ZHANG, Ophir SHALEM, Neville Espi SANJANA, John DOENCH, David ROOT, Ella Nicole BIEWENER HARTENIAN
  • Patent number: 10781444
    Abstract: The present invention generally relates to libraries, compositions, methods, applications, kits and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: September 22, 2020
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Feng Zhang, Ophir Shalem, Neville Espi Sanjana, John Doench, David Root, Ella Nicole Biewener Hartenian
  • Publication number: 20200263166
    Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
    Type: Application
    Filed: April 19, 2017
    Publication date: August 20, 2020
    Inventors: Feng ZHANG, Bernd ZETSCHE, Winston YAN, Neville Espi SANJANA, Sara JONES
  • Publication number: 20200208141
    Abstract: Described are methods comprises transducing a mammalian cell with one or more virus vectors. Each vector comprises a nucleic acid sequence encoding a Cpf1 (also known as Cas12a) protein and an optional selectable marker in operative association with an RNA pol II promoter which controls expression thereof; and a CRISPR RNA (crRNA) array comprising at least two spacers in operative association with an RNA pol III promoter. Each spacer encodes an RNA guide which hybridizes to a unique sequence located 3? from a T-rich protospacer-adjacent motif (PAM) in a genomic region of interest. The method further comprises culturing the transduced cells, thereby providing a plurality of cultured cell cultures, each cell culture comprising said deletion. Additionally, described are compositions used in methods as well as libraries generated by the methods. Such compositions comprise libraries of transduced cell cultures, viral vectors, nucleic acid sequences, CRISPR RNA spacers, and RNA guides, as described herein.
    Type: Application
    Filed: March 2, 2020
    Publication date: July 2, 2020
    Applicants: NEW YORK GENOME CENTER, INC., NEW YORK UNIVERSITY
    Inventor: Neville Espi SANJANA
  • Publication number: 20200165601
    Abstract: The present invention generally relates to compositions, methods applications and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.
    Type: Application
    Filed: July 29, 2019
    Publication date: May 28, 2020
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology
    Inventors: Feng ZHANG, Neville Espi Sanjana, Ophir Shalem
  • Publication number: 20200080094
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Application
    Filed: August 7, 2019
    Publication date: March 12, 2020
    Inventors: Feng Zhang, Le Cong, David Benjamin Turitz Cox, Patrick Hsu, Shuailiang Lin, Fei Ran, Randall Jeffrey Platt, Neville Espi Sanjana
  • Publication number: 20200063147
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Application
    Filed: August 5, 2019
    Publication date: February 27, 2020
    Inventors: Feng Zhang, Le Cong, David Benjamin Turitz Cox, Patrick Hsu, Shuailiang Lin, Fei Ran, Randall Jeffrey Platt, Neville Espi Sanjana
  • Publication number: 20200032277
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Application
    Filed: June 18, 2019
    Publication date: January 30, 2020
    Inventors: Feng Zhang, Le Cong, David Benjamin Turitz Cox, Patrick Hsu, Shuailiang Lin, Fei Ran, Randall Jeffrey Platt, Neville Espi Sanjana
  • Publication number: 20200032278
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Application
    Filed: June 18, 2019
    Publication date: January 30, 2020
    Inventors: Feng Zhang, Le Cong, David Benjamin Turitz Cox, Patrick Hsu, Shuailiang Lin, Fei Ran, Randall Jeffrey Platt, Neville Espi Sanjana
  • Publication number: 20190040399
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/CAS system.
    Type: Application
    Filed: April 30, 2018
    Publication date: February 7, 2019
    Inventors: Feng ZHANG, Le CONG, David Benjamin Turitz COX, Patrick HSU, Shuailiang LIN, Fei RAN, Randall Jeffrey PLATT, Neville Espi SANJANA
  • Publication number: 20190017058
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/CAS system.
    Type: Application
    Filed: April 30, 2018
    Publication date: January 17, 2019
    Inventors: Feng Zhang, Le CONG, David Benjamin Turitz COX, Patrick HSU, Shuailiang LIN, Fei RAN, Randall Jeffrey PLATT, Neville Espi SANJANA
  • Publication number: 20180327756
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Application
    Filed: August 5, 2016
    Publication date: November 15, 2018
    Inventors: Feng Zhang, Le Cong, David Benjamin Turitz Cox, Patrick Hsu, Shuailiang Lin, Fei Ran, Randall Jeffrey Platt, Neville Espi Sanjana
  • Publication number: 20180179547
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Application
    Filed: February 2, 2018
    Publication date: June 28, 2018
    Inventors: Feng Zhang, David Benjamin Turitz Cox, Luciano Marraffini, David Olivier Bikard, Wenyan Jiang, Neville Espi Sanjana
  • Publication number: 20180119138
    Abstract: The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.
    Type: Application
    Filed: November 8, 2017
    Publication date: May 3, 2018
    Inventors: Daniel E. Bauer, Stuart H. Orkin, Neville Espi Sanjana, Ophir Shalem, Jason Wright, Feng Zhang
  • Publication number: 20180112255
    Abstract: The present invention relates to in vivo methods for modeling tumor formation and/or tumor evolution comprising the use of eukaryotic cells in which one or more genetic target locus has been altered by the CRISPR/Cas system, and which cells are transplanted in non-human eukaryote as a model system for tumor formation and tumor evolution. In particular in vivo genetic screening methods for identifying genes involved in tumorigenesis and metastasis are disclosed. The invention further relates to kits and components for practicing the methods, as well as materials obtainable by the methods, in particular tumor and metastasis samples and cells or cell lines derived therefrom. The invention also relates to diagnostic and therapeutic methods derived from the information obtained in the modeling methods.
    Type: Application
    Filed: June 30, 2017
    Publication date: April 26, 2018
    Inventors: Sidi Chen, Randall Jeffrey Platt, Neville Espi Sanjana, Phillip A. Sharp, Feng Zhang
  • Patent number: 9822372
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Grant
    Filed: January 7, 2016
    Date of Patent: November 21, 2017
    Assignees: The Broad Institute Inc., Massachusetts Institute of Technology, The Rockefeller University
    Inventors: Feng Zhang, David Benjamin Turitz Cox, Luciano Marraffini, David Olivier Bikard, Wenyan Jiang, Neville Espi Sanjana
  • Publication number: 20170166903
    Abstract: The present invention generally relates to methods and compositions used for the spatial and temporal control of gene expression that may use inducible transcriptional effectors. The invention particularly relates to inducible methods of altering or perturbing expression of a genomic locus of interest in a cell wherein the genomic locus may be contacted with a non-naturally occurring or engineered composition comprising a deoxyribonucleic acid (DNA) binding polypeptide.
    Type: Application
    Filed: December 22, 2016
    Publication date: June 15, 2017
    Inventors: Feng Zhang, Mark Brigham, Le Cong, Silvana Konermann, Neville Espi Sanjana