Abstract: Methods and systems for determining a minimum number of cell line clones necessary to produce a product having a set of target product attributes are disclosed. An example method includes generating at least one cell line capable of expressing a polypeptide; measuring, using one or more analytical instruments, a plurality of measured product attribute values of a plurality of clones of a candidate cell line; receiving inputs, via a user interface, representing a set of target product attribute values for a product; projecting, by one or more processors based upon the plurality of measured values, a minimum number of subject clones of the product using the candidate cell line necessary to produce a subset of the subject clones having product attributes that satisfy one or more conditions associated with the set of target values; and generating the projected minimum number of subject clones of the product using the candidate cell line.

Abstract: The present disclosure provides a hydrogel composition comprising an interpenetrating polymer network (IPN) containing a biopolymer, a first synthetic polymer and a second synthetic polymer in which a contained community of live human MSCs is embedded. The collagen polymer matrix described (a) allows the embedded cells to remain in place or to migrate over short distances; (b) allows diffusion of small molecules, particularly growth factors produced by the cells or provided as a supplement, and EVs released by the cells to support the recovery of periodontium tissue function following injury.

Abstract: A thin film deposition method may include preparing a substrate structure having an opening region formed in a vertical direction and a plurality of holes formed in a horizontal direction in each of two side portions exposed by the opening region, and adsorbing an inhibitor to surfaces of the substrate structure so that an adsorption density of the inhibitor outside of the plurality of holes is higher than an adsorption density inside of the plurality of holes by adsorbing the inhibitor in a deposition environment in which a gas diffusivity is larger in the vertical direction than in the horizontal direction. A deposition process of a material film on the inside and outside of the plurality of holes is then performed, wherein a deposition rate of the material film may vary according to the adsorption density of the inhibitor.

Abstract: A thin film deposition method and a method of fabricating an electronic device using the same are disclosed. The thin film deposition method may include preparing a substrate structure having a pattern portion including a hole, adsorbing a reaction inhibitor to inside and outside of the hole in the substrate structure, wherein an adsorption density of the reaction inhibitor may be lower in the inside than the outside, and depositing a metal layer on the inside and outside the hole by an atomic layer deposition (ALD) process, wherein a deposition rate of the depositing may vary depending on regions by the reaction inhibitor, and wherein the reaction inhibitor may include a metal atom and a ligand for reaction inhibition bonded to the metal atom, and the metal atom may remain on the substrate structure in the depositing the metal layer.

Abstract: The present invention relates to a contrast agent suitable for ex vivo imaging, particularly of vascular structures and renal tubular structures, and a method for ex vivo imaging. The contrast agent is a polymer comprising monomers M. The monomer comprises a backbone having 2 to 6 elements, wherein at least one element is —CH(R)— or —N(R)—. R is a moiety -E-H, -L-(NH2)m or a moiety of formula 1, with E, L, R1 and R2 being defined as described in the present specification. The monomer comprises at least one —I to allow detection via X-ray and at least one —NH2 to allow crosslinking. Furthermore, the monomer comprises polar functional groups that contribute to water solubility. To avoid extravasation and glomerular filtration, the polymers are pre-crosslinked before the vasculature of a tissue, an organ or whole animal is perfused. After perfusion, the pre-crosslinked contrast agent is further crosslinked to be retained within the tissue, organ or animal permanently.

Abstract: A compound includes a polymer associated with a biological agent. The polymer has a first (meth)acryl monomeric unit with a cationic functional group R1 and a second (meth)acryl monomeric unit with a neutral hydrophilic functional group R2. The cationic functional group R1 is chosen from amino groups and alkylamino groups, and the neutral functional group R2 is chosen from polyethylene glycol (PEG), hydroxyl (OH), phosphorylcholine (PC), and mixtures and combinations thereof.

Abstract: Methods producing a recombinant protein of interest in a mammalian cell culture in media lacking IGF-1 are provided. Methods for producing mammalian cells capable of growing in media lacking IGF-1 are also provided.

Abstract: A corneal device comprising a base surface and a top surface, and having a water content ranging from about 78%-92% (w/w), inclusive. The corneal device can be used to treat, for example without limitation, ectatic corneal disease by supporting corneal structure weakened by the disease and/or changing an anterior curvature of the cornea.

Abstract: An aqueous solution concentrate of glufosinate-ammonium comprising; at least 28 wt % glufosinate-ammonium; at least one alkylether sulfate surfactant; at least one alkylglycoside; and propylene glycol monomethyl ether.

Abstract: PiggyBac transposases engineered to increase stability in a cell. The engineered piggyBac transposases are useful for stably transforming cells, cell line development, genome modification, and improving titer of recombinant proteins, among other uses.

Abstract: Embodiments of the present disclosure provide for composite materials, methods of making composite materials, methods of using composite materials, and the like. In particular, the present application relates to hollow fibers and to pressure-retarded osmosis systems comprising said fibers. The hollow fibers have an inside layer and an outside layer, wherein the outside layer covers an outside surface of the inside layer, wherein the inside layer forms a boundary around the lumen, wherein the inside layer includes a bi-layer structure, wherein the bi-layer structure includes a sponge-like layer and a finger-like layer, wherein the sponge-like layer is disposed closer to the lumen of the hollow fiber and the finger-like layer is disposed on the sponge-like layer on the side opposite the lumen, wherein the outside layer includes a polyamide layer.

Abstract: Corneal device inserters and positioners that include an elongate member with a distal region, the distal region including a top portion, a bottom portion, and a corneal device receiving space, the corneal device receiving space defined by the top portion, the bottom portion, and a receiving space proximal end, the distal region further comprising a slot in fluid communication with the corneal device receiving space, the slot extending through at least one of the top portion and the bottom portion, the slot having a slot proximal end disposed at least as far proximally as the receiving space proximal end.

Abstract: Embodiments described herein relate to a faceplate for improving film uniformity. A semiconductor processing apparatus includes a pedestal, an edge ring and a faceplate having distinct regions with differing hole densities. The faceplate has an inner region and an outer region which surrounds the inner region. The inner region has a greater density of holes formed therethrough when compared to the outer region. The inner region is sized to correspond with a substrate being processed while the outer region is sized to correspond with the edge ring.

Abstract: Embodiments of the present disclosure provide for composite materials, methods of making composite materials, methods of using composite materials, and the like. In particular, the present application relates to hollow fibers and to pressure-retarded osmosis systems comprising said fibers. The hollow fibers have an inside layer and an outside layer, wherein the outside layer covers an outside surface of the inside layer, wherein the inside layer forms a boundary around the lumen, wherein the inside layer includes a bi-layer structure, wherein the bi-layer structure includes a sponge-like layer and a finger-like layer, wherein the sponge-like layer is disposed closer to the lumen of the hollow fiber and the finger-like layer is disposed on the sponge-like layer on the side opposite the lumen, wherein the outside layer includes a polyamide layer.

Abstract: Corneal implant retaining devices and their methods of use. The retaining devices can be a cap adapted to be disposed over a portion of a corneal implant insertion device.

Abstract: Methods and compositions are provided for the efficient and beneficial use of nanoparticulates, such as carbon nanotubes. In various embodiments, a nanoparticulate entrapped in a linear polymer, where the linear polymer is formed in the presence of the nanoparticulate, is provided. The entrapped nanoparticulate provides an efficient means to introduce nanoparticulates into compositions, such as resins and fiber-reinforced resins, allowing for increased dispersion and beneficial properties.

Abstract: A method of matching geographic information to a requester including matching users subscribed to a service with devices used by the users; receiving and storing geographic information corresponding to locations of the users; receiving content selectively authorized by the users to be shared, and matching the content with the geographic information corresponding to the locations of the users; receiving from a requester a request specific to a location indicated on an electronic map and identifying from the content selectively authorized by the users and based on matched geographic information that matches the location indicated on the electronic map, a subset of the content to provide to the requester in response to the request; and providing, over a communications network, the subset of the content to provide to the requester in response to the request and based on the geographic information that matches the location indicated on the electronic map.

Abstract: A training cornea adapted to train physicians how to position a corneal inlay within a cornea, and methods thereof. The training cornea having a pre-made flap therein and having a base curvature with a radius of curvature that is the same as the radius of curvature training ball. The training cornea comprising a hydrophilic material.

Abstract: Devices that are adapted to insert corneal implants onto corneal tissue. Methods of using the devices include inserting the corneal implant into a pocket created in the cornea. Methods of use also include inserting the corneal implant onto corneal tissue after a flap has been created in the cornea. The devices can be adapted to deliver fluid to an implant holding space to at least assist in the deployment of the implant from the holding space and onto corneal tissue.