Abstract: A gene editing system of Candida viswanathii includes a Candida viswanathii, a first gene editing fragment and a second gene editing fragment. The first gene editing fragment successively includes a first homology arm and a screening gene. The second gene editing fragment is connected to a C-terminus of the first gene editing fragment and includes a second homology arm, a Cas9 expression cassette and a sgRNA cassette. The Cas9 expression cassette successively includes a Cas9 promoter, a Cas9 gene and three nuclear localization sequences. The sgRNA cassette successively includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment for gene editing of a chromosome of the Candida viswanathii.

Abstract: An apparatus includes a captured carbon dioxide input. The captured carbon dioxide input is coupled to receive captured carbon dioxide from a direct air capture system. The apparatus uses the captured carbon dioxide as a low global warming refrigerant to provide cooling functionality in automotive, commercial, and industrial applications, or other operations involving low global warming refrigerants. In various embodiments, the apparatus is a refrigeration apparatus or a heat pump apparatus. Low global warming carbon dioxide refrigerant is natural, non-toxic, non-flammable, and abundant when obtained from a direct air capture system. Moreover, carbon dioxide refrigerant has a high heat transfer coefficient and has a global warming potential (GWP) of one. Carbon dioxide refrigerant is a more sustainable and efficient coolant option than common refrigerants, such as R22, R152, R404a, and R1234yf refrigerants.

Abstract: A sensor communications system provides for the secure transfer of sensor measurement data from sensor devices to a remote, network connected server system. The sensor devices include processor to encrypt a sensor measurement data stream using the public key of an asymmetric key pair and to transmit the encrypted sensor measurement data stream through the local network interface. The remote server system includes a network gateway and a compute server with the compute server being couplable to the network gateway and an authorization server storing the private key of the asymmetric key pair. The compute server operates to decrypt the encrypted sensor measurement data stream using the private key and to process the decrypted sensor measurement data stream for presentation a user of the remote server system.

Abstract: Low-load-modulation, broadband power amplifiers and method of use are described. The amplifiers can include multiple amplifiers connected in parallel to amplify a signal that has been divided into parallel circuit branches. One of the amplifiers can operate as a main amplifier in a first amplification class and the remaining amplifiers can operate as peaking amplifiers in a second amplification class. The main amplifier can see low modulation of its load between the fully-on and fully backed-off states of the amplifier. With lower load modulation, the power amplifiers described herein exhibit better power-handling capability and RF fractional bandwidth as compared to conventional amplifiers.

Abstract: The present invention relates to a propeller control mechanism comprising a center, a first group, and a second group. This mechanism is capable of controlling the angle of attack of the propeller blades without auxiliary actuators or external linkages and minimizing the propeller blades' motion range. Furthermore, this mechanism is applicable not only to aerial vehicles but also to vehicles on the surface of water or underwater.

Abstract: An overcap assembly includes an actuating button attached to and surrounded by the body. The actuating button has a fluid passageway therein, and the fluid passageway is configured to receive a fluid when the actuating button is depressed. The overcap assembly further includes a first nozzle and a second nozzle. The first nozzle extends from the actuating button and is in fluid communication with the fluid passageway. The first nozzle includes a first exit aperture. The second nozzle extends from the actuating button and is positioned below the first nozzle. The second nozzle is in fluid communication with the fluid passageway. The second nozzle includes a second exit aperture angled differently than the first exit aperture. The first nozzle and the second nozzle each comprise an inner cylindrical wall and an outer cylindrical wall surrounding and spaced apart from the inner cylindrical wall.

Abstract: An overcap assembly configured to attach to a container. The overcap assembly includes a body, an actuator, a first nozzle, and a second nozzle. The actuator is integrally attached with the body and defines a longitudinal axis. The actuator further comprises a fluid passageway that extends therein. The first nozzle and the second nozzle extend laterally from the actuator, and the first nozzle and the second nozzle define a portion of the fluid passageway. The first nozzle comprises a first angled exit aperture and the second nozzle comprises a second angled exit aperture. The first nozzle and the second nozzle each comprises an inner cylindrical wall and an outer cylindrical wall surrounding and spaced apart from the inner cylindrical wall. The first angled exit aperture and the second angled exit aperture are non-parallel to the longitudinal axis.

Abstract: An efficient low-energy carbon dioxide removal system comprises an automated air mover equipped with sensing devices to measure flow rate, volume, level, pressure, temperature and concentration. Packing materials and air-liquid distributors are used in a multi-stage catalytic reactor. The multi-stage catalytic reactor processes ambient air and generates pure carbon dioxide gas and generates exhausted gas released to ambient air. In operation, air contacts the base solution in the presence of a catalyst via the air mover, distributor, and packing materials. The air reacts with the base solution thereby generating a base solution having carbon dioxide and generating exhaust (absorption reaction). Next, the exhaust is released from the reactor. Next, a catalyst is added, heat is applied to the base solution having carbon dioxide thereby generating carbon dioxide and generating a base solution without carbon dioxide (desorption reaction).

Abstract: A networked computer system providing an automated control system that enables a sensor client to securely communicate with a heterogeneous set of unique sensors. The system enables autonomous secure identification of specific sensors capable of system inter-operation. Recognized sensors are autonomously paired and the connection secured using a password unique to the sensor and autonomously managed by the system. Measurement data is source encrypted using a well-defined public key, thereby providing data security that extends uninterrupted from the sensor itself to an appropriately authorized subsystem of the control system.

Abstract: Apparatus and methods for a low-load-modulation power amplifier are described. Low-load-modulation power amplifiers can include multiple amplifiers connected in parallel to amplify a signal that has been divided into parallel circuit branches. One of the amplifiers can operate as a main amplifier in a first amplification class and the remaining amplifiers can operate as peaking amplifiers in a second amplification class. The main amplifier can see low modulation of its load between the power amplifier's fully-on and fully backed-off states. Improvements in bandwidth and drain efficiency over conventional Doherty amplifiers are obtained.

Abstract: An apparatus includes a captured carbon dioxide input. The captured carbon dioxide input is coupled to receive captured carbon dioxide from a direct air capture system. The apparatus uses the captured carbon dioxide as a low global warming refrigerant to provide cooling functionality in automotive, commercial, and industrial applications, or other operations involving low global warming refrigerants. In various embodiments, the apparatus is a refrigeration apparatus or a heat pump apparatus. Low global warming carbon dioxide refrigerant is natural, non-toxic, non-flammable, and abundant when obtained from a direct air capture system. Moreover, carbon dioxide refrigerant has a high heat transfer coefficient and has a global warming potential (GWP) of one. Carbon dioxide refrigerant is a more sustainable and efficient coolant option than common refrigerants, such as R22, R152, R404a, and R1234yf refrigerants.

Abstract: An efficient low-energy carbon dioxide removal system comprises an automated air mover equipped with sensing devices to measure flow rate, volume, level, pressure, temperature and concentration. Packing materials and air-liquid distributors are used in a multi-stage catalytic reactor. The multi-stage catalytic reactor processes ambient air and generates pure carbon dioxide gas and generates exhausted gas released to ambient air. In operation, air contacts the base solution in the presence of a catalyst via the air mover, distributor, and packing materials. The air reacts with the base solution thereby generating a base solution having carbon dioxide and generating exhaust (absorption reaction). Next, the exhaust is released from the reactor. Next, a catalyst is added, heat is applied to the base solution having carbon dioxide thereby generating carbon dioxide and generating a base solution without carbon dioxide (desorption reaction).

Abstract: An apparatus includes a captured carbon dioxide input. The captured carbon dioxide input is coupled to receive captured carbon dioxide from a direct air capture system. The apparatus uses the captured carbon dioxide as a low global warming refrigerant to provide cooling functionality in automotive, commercial, and industrial applications, or other operations involving low global warming refrigerants. In various embodiments, the apparatus is a refrigeration apparatus or a heat pump apparatus. Low global warming carbon dioxide refrigerant is natural, non-toxic, non-flammable, and abundant when obtained from a direct air capture system. Moreover, carbon dioxide refrigerant has a high heat transfer coefficient and has a global warming potential (GWP) of one. Carbon dioxide refrigerant is a more sustainable and efficient coolant option than common refrigerants, such as R22, R152, R404a, and R1234yf refrigerants.

Abstract: The present disclosure relates to a transformant for producing 2,5-furandicarboxylic acid. The transformant for producing 2,5-furandicarboxylic acid includes a Pseudomonas putida and at least one exogenous gene. The exogenous gene is an HmfH gene or an HMFO gene, and the exogenous gene is integrated into the chromosome of the Pseudomonas putida.

Abstract: An overcap assembly configured to attach to a container. The overcap assembly includes a body, an actuator, a first nozzle, and a second nozzle. The actuator is integrally attached with the body and defines a longitudinal axis. The actuator further comprises a fluid passageway that extends therein. The first nozzle and the second nozzle extend laterally from the actuator, and the first nozzle and the second nozzle define a portion of the fluid passageway. The first nozzle comprises a first angled exit aperture and the second nozzle comprises a second angled exit aperture. The first nozzle and the second nozzle each comprises an inner cylindrical wall and an outer cylindrical wall surrounding and spaced apart from the inner cylindrical wall. The first angled exit aperture and the second angled exit aperture are non-parallel to the longitudinal axis.

Abstract: Apparatus and methods for a no-load-modulation power amplifier are described. No-load-modulation power amplifiers can comprise multiple amplifiers connected in parallel to amplify a signal that has been divided into parallel circuit branches. One of the amplifiers can operate as a main amplifier in a first amplification class and the remaining amplifiers can operate as peaking amplifiers in a second amplification class. The main amplifier can see essentially no modulation of its load between the power amplifier's fully-on and fully backed-off states. The power amplifiers can operate in symmetric and asymmetric modes. Improvements in bandwidth and drain efficiency over conventional Doherty amplifiers are obtained. Further improvements can be obtained by combining signals from the amplifiers with hybrid couplers.

Abstract: Apparatus and methods for a no-load-modulation power amplifier are described. No-load-modulation power amplifiers can comprise multiple amplifiers connected in parallel to amplify a signal that has been divided into parallel circuit branches. One of the amplifiers can operate as a main amplifier in a first amplification class and the remaining amplifiers can operate as peaking amplifiers in a second amplification class. The main amplifier can see essentially no modulation of its load between the power amplifier's fully-on and fully backed-off states. The power amplifiers can operate in symmetric and asymmetric modes. Improvements in bandwidth and drain efficiency over conventional Doherty amplifiers are obtained. Further improvements can be obtained by combining signals from the amplifiers with hybrid couplers.

Abstract: The present invention provides a liquid eliminating cartridge that is formed by snapping two mating pieces together. The pieces may include a body portion and a top portion and may together clamp an oleophobic/hydrophobic membrane there between.

Abstract: Secure communication with a server can be reestablished after the server's certificate is renewed with a certificate authority unknown to a client. When a server's certificate is renewed with a CA whose certificate does not exist in a client's certificate store, the client will not be able to authenticate the server using SSL unless the CA's certificate is added to the certificate store. When a client is not updated to include the CA's certificate and therefore fails to authenticate the server, a web-based application on the client can perform a fallback authentication process to securely and automatically add any necessary CA certificates to the certificate store thereby eliminating the need for an administrator to manually install the necessary CA certificates.

Abstract: Low-load-modulation, broadband power amplifiers and method of use are described. The amplifiers can include multiple amplifiers connected in parallel to amplify a signal that has been divided into parallel circuit branches. One of the amplifiers can operate as a main amplifier in a first amplification class and the remaining amplifiers can operate as peaking amplifiers in a second amplification class. The main amplifier can see low modulation of its load between the fully-on and fully backed-off states of the amplifier. With lower load modulation, the power amplifiers described herein exhibit better power-handling capability and RF fractional bandwidth as compared to conventional amplifiers.